Human G Protein Coupled Receptor 37 ELISA Kit | GPR37 elisa kit

Human G Protein Coupled Receptor 37 ELISA Kit

Cat. Num. AAA760642

• Gene Names: GPR37; PAELR; EDNRBL; hET(B)R-LP
• Reactivity: Human
• Synonyms: G Protein Coupled Receptor 37; Human G Protein Coupled Receptor 37 ELISA Kit; GPR37/ETBR-LP-1/PAELR/EDNRBL/Endothelin B receptor-like protein 1/G protein-coupled receptor 37 (endothelin receptor type B-like)/hET (B)R-LP/PAELRETBR-LP-1/Parkin-associated endothelin receptor-like receptor/probable G-protein coupled receptor 37; GPR37 elisa kit

• Reactivity: Human
• Sequence Length: 613

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 0.156-10ng/ml
• Sensitivity: 0.094ng/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for GPR37 elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. anti-Human GPR37 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human GPR37 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human GPR37 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Human GPR37 can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 3892879
• NCBI GeneID: 2861
• UniProt Accession #: O15354; O00348; O14768; Q8TD39; A4D0Y6
• Molecular Weight: 67,114 Da
• NCBI Official Full Name: G protein coupled receptor 37
• NCBI Official Synonym Full Names: G protein-coupled receptor 37 (endothelin receptor type B-like)
• NCBI Official Symbol: GPR37
• NCBI Official Synonym Symbols: PAELR; EDNRBL; hET(B)R-LP
• NCBI Protein Information: prosaposin receptor GPR37
• UniProt Protein Name: Prosaposin receptor GPR37
• Protein Family: Prosaposin receptor
• UniProt Gene Name: GPR37
• UniProt Synonym Gene Names: ETBR-LP-1; PAELR
• UniProt Entry Name: GPR37_HUMAN

NCBI Description

This gene is a member of the G protein-coupled receptor family. The encoded protein contains seven transmembrane domains and is found in cell and endoplasmic reticulum membranes. G protein-coupled receptors are involved in translating outside signals into G protein mediated intracellular effects. This gene product interacts with Parkin and is involved in juvenile Parkinson disease. [provided by RefSeq, Oct 2012]

Uniprot Description

GPR37: Orphan receptor. May have a unique functional role in the central nervous system. Belongs to the G-protein coupled receptor 1 family.

Protein type: Receptor, GPCR; GPCR, family 1; Membrane protein, multi-pass; Membrane protein, integral

Chromosomal Location of Human Ortholog: 7q31

Cellular Component: endoplasmic reticulum; endoplasmic reticulum membrane; integral to plasma membrane; plasma membrane; receptor complex; ubiquitin ligase complex

Molecular Function: G-protein coupled receptor activity; heat shock protein binding; Hsp70 protein binding; peptide binding; peptide receptor activity, G-protein coupled; protein binding; ubiquitin protein ligase binding

Biological Process: dopamine biosynthetic process; G-protein coupled receptor protein signaling pathway; G-protein signaling, adenylate cyclase inhibiting pathway; locomotion during locomotory behavior; positive regulation of dopamine metabolic process; positive regulation of MAPKKK cascade