Rabbit RPS8 Polyclonal Antibody | anti-RPS8 antibody

RPS8 (R152) Polyclonal Antibody

Cat. Num. AAA3004302

• Gene Names: RPS8; S8
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: RPS8; Polyclonal Antibody; RPS8 (R152) Polyclonal Antibody; 40S ribosomal protein S8; OK/SW-cl.83; Ribosomal protein S8; anti-RPS8 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Ribosomal Protein S8 (R152) polyclonal antibody detects endogenous levels of Ribosomal Protein S8 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 208

• Applicable Applications for anti-RPS8 antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: WB: 1:500-1:1000; IHC: 1:50-1:200
• Immunogen: Synthetic peptide, corresponding to amino acids 120-170 of Human Ribosomal Protein S8.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of RPS8 (R152) pAb at 1:500 dilutionLane1:AML-12 whole cell lysate (40ug) Lane2:PC12 whole cell lysate (40ug) Lane3:A549 whole cell lysate (40ug) Lane4:H1792 whole cell lysate (40ug) Lane5:HEK293 whole cell lysate (40ug))

Related Product Information for anti-RPS8 antibody

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 40S subunit. The protein belongs to the S8E family of ribosomal proteins. It is located in the cytoplasm. Increased expression of this gene in colorectal tumors and colon polyps compared to matched normal colonic mucosa has been observed. This gene is co-transcribed with the small nucleolar RNA genes U38A, U38B, U39, and U40, which are located in its fourth, fifth, first, and second introns, respectively. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome.

NCBI and Uniprot Product Information

• NCBI GI #: 4506743
• NCBI GeneID: 6202
• NCBI Accession #: NP_001003.1
• NCBI GenBank Nucleotide #: NP_001003.1
• UniProt Accession #: P62241
• Molecular Weight: ~ 30kDa
• NCBI Official Full Name: 40S ribosomal protein S8
• NCBI Official Synonym Full Names: ribosomal protein S8
• NCBI Official Symbol: RPS8
• NCBI Official Synonym Symbols: S8
• NCBI Protein Information: 40S ribosomal protein S8
• UniProt Protein Name: 40S ribosomal protein S8
• Protein Family: 30S ribosomal protein
• UniProt Gene Name: RPS8
• UniProt Entry Name: RS8_HUMAN

NCBI Description

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 40S subunit. The protein belongs to the S8E family of ribosomal proteins. It is located in the cytoplasm. Increased expression of this gene in colorectal tumors and colon polyps compared to matched normal colonic mucosa has been observed. This gene is co-transcribed with the small nucleolar RNA genes U38A, U38B, U39, and U40, which are located in its fourth, fifth, first, and second introns, respectively. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq, Jul 2008]

Uniprot Description

RPS8: a ribosomal protein that is a component of the 40S subunit. The protein belongs to the S8E family of ribosomal proteins. It is located in the cytoplasm. Increased expression of this gene in colorectal tumors and colon polyps compared to matched normal colonic mucosa has been observed. This gene is co-transcribed with the small nucleolar RNA genes U38A, U38B, U39, and U40, which are located in its fourth, fifth, first, and second introns, respectively. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq, Jul 2008]

Protein type: Ribosomal; Translation

Chromosomal Location of Human Ortholog: 1p34.1-p32

Cellular Component: focal adhesion; membrane; cytoplasm; ribonucleoprotein complex; cytosol; nucleus

Molecular Function: structural constituent of ribosome

Biological Process: maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA); SRP-dependent cotranslational protein targeting to membrane; translational elongation; cellular protein metabolic process; translation; viral reproduction; mRNA catabolic process, nonsense-mediated decay; translational initiation; gene expression; viral transcription; translational termination; viral infectious cycle