Human Probable G-protein coupled receptor 21 ELISA Kit | GPR21 elisa kit

Human Probable G-protein coupled receptor 21 (GPR21) ELISA Kit

Cat. Num. AAA281674

• Reactivity: Human
• Synonyms: Probable G-protein coupled receptor 21; Human Probable G-protein coupled receptor 21 (GPR21) ELISA Kit; GPR21 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Human GPR21. No significant cross-reactivity or interference between Human GPR21 and analogues was observed.
• Sequence Length: 349

• Samples: Serum, Plasma, Other biological fluids
• Assay Type: Sandwich
• Sample Volume: 1-200 uL
• Precision: Intra-assay Precision (Precision within an assay); Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.; Inter-assay Precision (Precision between assays); Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.; CV (%) = SD/meanX100; Intra-Assay: CV; Inter-Assay: CV
• Detection Wavelength: 450 nm
• Preparation and Storage: Store at 2-8 degree C.

Related Product Information for GPR21 elisa kit

O'Dowd et al. (1997) mapped the GPR21 gene to 9q33 by fluorescence in situ hybridization.O'Dowd et al. (1997) reported the discovery of 4 novel human putative G protein-coupled receptor (GPR) genes. By screening an EST database, they identified cDNA sequences that partially encode 3 additional GPRs. These cDNA fragments were used to screen a genomic library to isolate the full-length coding region of the genes. This resulted in the isolation of genes GPR21, GPR22, and GPR23. The 4 encoded receptors share significant identity to each other and to other members of the receptor family.
By fluorescence in situ hybridization, O'Dowd et al. (1997) mapped GPR20 to 8q24.2-q24.3, GPR21 to 9q33, GPR22 to 7q22-q31.1, and GPR23 to Xq13-q21.1.

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate GPR21 in samples. An antibody specific for GPR21 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyGPR21 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for GPR21 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of GPR21 bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 4885307
• NCBI GeneID: 2844
• NCBI Accession #: NP_005285.1
• NCBI GenBank Nucleotide #: NM_005294.2
• UniProt Accession #: Q99679; Q6NXU2; B2R8W9
• Molecular Weight: 39,515 Da
• NCBI Official Full Name: probable G-protein coupled receptor 21
• NCBI Official Synonym Full Names: G protein-coupled receptor 21
• NCBI Official Symbol: GPR21
• NCBI Protein Information: probable G-protein coupled receptor 21
• UniProt Protein Name: Probable G-protein coupled receptor 21
• Protein Family: Probable G-protein coupled receptor
• UniProt Gene Name: GPR21

NCBI Description

This gene encodes a member of the G-protein-coupled receptor 1 family. G-protein coupled receptors are membrane proteins which activate signaling cascades as a response to extracellular stress. The encoded protein activates a Gq signal transduction pathway which mobilizes calcium. [provided by RefSeq, Nov 2012]

Uniprot Description

GPR21: Orphan receptor. Belongs to the G-protein coupled receptor 1 family.

Protein type: GPCR, family 1; Membrane protein, integral; Membrane protein, multi-pass; Receptor, GPCR

Chromosomal Location of Human Ortholog: 9q33.2

Cellular Component: integral component of membrane; plasma membrane

Molecular Function: dopamine D1 receptor-like receptor activity

Biological Process: dopamine receptor signaling pathway; glucose homeostasis; negative regulation of insulin receptor signaling pathway; positive regulation of multicellular organism growth