Glycoprotein Non-Metastatic Melanoma Protein B Recombinant Protein | GPNMB recombinant protein

Recombinant Human GPNMB Protein (aa 22-486, His Tag)

Cat. Num. AAA2556672

• Gene Names: GPNMB; NMB; HGFIN; PLCA3
• Purity: > 95 % as determined by reducing SDS-PAGE.
• Synonyms: Glycoprotein Non-Metastatic Melanoma Protein B; Recombinant Human GPNMB Protein (aa 22-486; His Tag); Transmembrane Glycoprotein NMB; Transmembrane Glycoprotein HGFIN; GPNMB; HGFIN; NMB; Osteoactivin; GPNMB recombinant protein

• Host: Human Cells
• Purity/Purification: > 95 % as determined by reducing SDS-PAGE.
• Form/Format: Lyophilized from a 0.2 um filtered solution of 20mM PB, 150mM NaCl, pH 7.2.
• Sequence: Ala22-Pro486

• Species: Human
• Endotoxin: <1.0 EU per ug as determined by LAL test.
• Tag: C-6His
• Reconstitution: We recommend that the vial be centrifuged to opening to bring the contents to the bottom. Do not mix by vortex. It is recommended that sterile water(200ul) be added to the vial to prepare a stock solution of 0.25mg/mL. Concentration is measured by UV-Vis.
• Preparation and Storage: Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8 degree C for 2-7 days. Aliquots of reconstituted samples are stable at < -20 degree C for 3 months.; Shipping: This product is provided as lyophilized powder which is shipped with blue ice.

SDS-Page

Related Product Information for GPNMB recombinant protein

Osteoactivin is an intracellular glycoprotein belongs to the NMB/pMEL-17 family, which is asscociated with cell endosomal/lysomal compartments. Human Osteoactivin is a 560 amino acid type I transmembrane protein, and one alternate splice form shows a 12 amino acid insert between amino acid 339-340. An additional 206 amino acid isoform shows a mutation at position 181 that results in a 26 amino acid substitution for the C-terminal 380 amino acids. Cells knowns to express Osteoactivin include fibroblast, osteoblasts, myeloid dendritic cell, melanocytes, plus fetal chondrocytes and stratum basale keratinocytes, macrophages/keratinocytes.

NCBI and Uniprot Product Information

• NCBI GI #: 52694752
• NCBI GeneID: 10457
• NCBI Accession #: NP_001005340.1
• NCBI GenBank Nucleotide #: NM_001005340.1
• UniProt Accession #: Q14956; Q6UVX1; Q8N1A1; A4D155
• Molecular Weight: Molecular Mass: 53.09 kDa; Actual Protein Molecular Mass: 80-120 kDa
• NCBI Official Full Name: transmembrane glycoprotein NMB isoform a
• NCBI Official Synonym Full Names: glycoprotein nmb
• NCBI Official Symbol: GPNMB
• NCBI Official Synonym Symbols: NMB; HGFIN; PLCA3
• NCBI Protein Information: transmembrane glycoprotein NMB
• UniProt Protein Name: Transmembrane glycoprotein NMB
• Protein Family: Transmembrane glycoprotein
• UniProt Gene Name: GPNMB
• UniProt Synonym Gene Names: HGFIN1 PublicationManual assertion based on opinion iniRef.2

NCBI Description

The protein encoded by this gene is a type I transmembrane glycoprotein which shows homology to the pMEL17 precursor, a melanocyte-specific protein. GPNMB shows expression in the lowly metastatic human melanoma cell lines and xenografts but does not show expression in the highly metastatic cell lines. GPNMB may be involved in growth delay and reduction of metastatic potential. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

Could be a melanogenic enzyme.