Human Glycoprotein hormone beta-5 ELISA Kit | GPHB5 elisa kit
Human Glycoprotein hormone beta-5 (GPHB5) ELISA Kit
Gene Names
GPHB5; B5; GPB5; ZLUT1
Reactivity
Human
Synonyms
Glycoprotein hormone beta-5; Human Glycoprotein hormone beta-5 (GPHB5) ELISA Kit; GPHB5 elisa kit
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of Human GPHB5. No significant cross-reactivity or interference between Human GPHB5 and analogues was observed.
Sequence Length
130
Samples
Serum, plasma and other biological fluids
Assay Type
Quantitative Sandwich
Detection Range
0.156 ng/mL - 10 ng/mL
Sensitivity
0.1 ng/mL
Intra-assay Precision
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision. Intra-Assay: CV<8%
Inter-assay Precision
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested in forty separate assays to assess inter-assay precision. CV (%) = SD/meanX100. Inter-Assay: CV<12%
Preparation and Storage
Store at 2-8 degree C.
Related Product Information for GPHB5 elisa kit
Intended Uses: For the quantitative detection of Human Glycoprotein hormone beta-5 (GPHB5) concentration in serum, plasma and other biological fluids.
Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate GPHB5 in Human serum, plasma. An antibody specific for GPHB5 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any GPHB5 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for GPHB5 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of GPHB5 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate GPHB5 in Human serum, plasma. An antibody specific for GPHB5 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any GPHB5 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for GPHB5 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of GPHB5 bound in the initial step. The color development is stopped and the intensity of the color is measured.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
14,232 Da
NCBI Official Full Name
glycoprotein hormone beta-5
NCBI Official Synonym Full Names
glycoprotein hormone beta 5
NCBI Official Symbol
GPHB5
NCBI Official Synonym Symbols
B5; GPB5; ZLUT1
NCBI Protein Information
glycoprotein hormone beta-5
UniProt Protein Name
Glycoprotein hormone beta-5
Protein Family
UniProt Gene Name
GPHB5
UniProt Synonym Gene Names
GPB5; ZLUT1
NCBI Description
GPHB5 is a cystine knot-forming polypeptide and a subunit of the dimeric glycoprotein hormone family (Hsu et al., 2002 [PubMed 12089349]).[supplied by OMIM, Mar 2008]
Uniprot Description
GPHB5: Stimulates the thyroid. Binds and activates THSR, leading to increased cAMP production. Belongs to the glycoprotein hormones subunit beta family.
Protein type: Secreted; Secreted, signal peptide
Chromosomal Location of Human Ortholog: 14q23.2
Cellular Component: cytoplasm; extracellular region; extracellular space
Molecular Function: hormone activity; protein heterodimerization activity; thyrotropin-releasing hormone receptor binding
Biological Process: cell surface receptor signaling pathway; cell-cell signaling; G-protein coupled receptor signaling pathway; hormone-mediated signaling pathway; positive regulation of cAMP biosynthetic process
Research Articles on GPHB5
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Product Notes
The Human GPHB5 gphb5 (Catalog #AAA280386) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA280386 ELISA Kit recognizes Human GPHB5. It is sometimes possible for the material contained within the vial of "Glycoprotein hormone beta-5, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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