anti-Human Glypican-1 Antibody | anti-GPC1 antibody

Goat anti-Glypican-1 Antibody

Cat. Num. AAA423795

• Gene Names: GPC1; glypican
• Reactivity: Human
• Applications: ELISA, Western Blot
• Purity: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Synonyms: Glypican-1; Goat anti-Glypican-1 Antibody; GPC1; glypican 1; glypican; glypican proteoglycan 1; glypican-1; anti-GPC1 antibody

• Reactivity: Human
• Purity/Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Form/Format: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
• Sequence Length: 558

• Applicable Applications for anti-GPC1 antibody: Peptide ELISA (EIA), Western Blot (WB)
• Application Notes: Pep-ELISA: antibody detection limit dilution 1:16000.; WB: Approx 60kDa band observed in lysates of cell lines HeLa and K562. Recommended concentration: 1-3ug/ml.
• Immunogen Sequence: Peptide with sequence C-TDKFWGTSGVES, from the internal region of the protein sequence according to NP_002072.2.
• Peptide Sequence + C: C-TDKFWGTSGVES
• Epitope: Internal Region
• Preparation and Storage: Aliquot and store at -20 degree C. Minimize freezing and thawing.

Immunohistochemistry (IHC)

((2ug/ml) staining of HeLa (A) and K562 (B) lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

NCBI and Uniprot Product Information

• NCBI GI #: 167001141
• NCBI GeneID: 2817
• NCBI Accession #: NP_002072.2
• NCBI GenBank Nucleotide #: NM_002081.2
• UniProt Accession #: P35052; Q53QM4; B3KTD1
• Molecular Weight: 61.7kDa
• NCBI Official Full Name: glypican-1
• NCBI Official Synonym Full Names: glypican 1
• NCBI Official Symbol: GPC1
• NCBI Official Synonym Symbols: glypican
• NCBI Protein Information: glypican-1
• UniProt Protein Name: Glypican-1
• Protein Family: Glypican
• UniProt Gene Name: GPC1

NCBI Description

Cell surface heparan sulfate proteoglycans are composed of a membrane-associated protein core substituted with a variable number of heparan sulfate chains. Members of the glypican-related integral membrane proteoglycan family (GRIPS) contain a core protein anchored to the cytoplasmic membrane via a glycosyl phosphatidylinositol linkage. These proteins may play a role in the control of cell division and growth regulation. [provided by RefSeq, Jul 2008]

Uniprot Description

Cell surface proteoglycan that bears heparan sulfate. Binds, via the heparan sulfate side chains, alpha-4 (V) collagen and participates in Schwann cell myelination (). May act as a catalyst in increasing the rate of conversion of prion protein PRPN(C) to PRNP(Sc) via associating (via the heparan sulfate side chains) with both forms of PRPN, targeting them to lipid rafts and facilitating their interaction. Required for proper skeletal muscle differentiation by sequestering FGF2 in lipid rafts preventing its binding to receptors (FGFRs) and inhibiting the FGF-mediated signaling.