Chicken Glucoprotein 130 ELISA Kit | gp130 elisa kit

Chicken Glucoprotein 130 ELISA Kit

Cat. Num. AAA734193

• Gene Names: IL6ST; CD130; GP130; CDW130; IL-6RB
• Reactivity: Chicken
• Synonyms: Glucoprotein 130; Chicken Glucoprotein 130 ELISA Kit; gp130 elisa kit

• Reactivity: Chicken

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Sensitivity: 1.0 ng/mL.
• Intended Uses: This gp130 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Chicken gp130. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for gp130 elisa kit

Principle of the assay: gp130 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-gp130 antibody and an gp130-HRP conjugate. The assay sample and buffer are incubated together with gp130-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the gp130 concentration since gp130 from samples and gp130-HRP conjugate compete for the anti-gp130 antibody binding site. Since the number of sites is limited, as more sites are occupied by gp130 from the sample, fewer sites are left to bind gp130-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The gp130 concentration in each sample is interpolated from this standard curve.

Product Categories/Family for gp130 elisa kit

Cell Biology

NCBI and Uniprot Product Information

• NCBI GI #: 1246098
• NCBI GeneID: 3572
• UniProt Accession #: Q13514; O15281; P40189; Q17RA0; Q5FC02; Q5FC03; Q5FC05; Q5FC06; Q5FC07
• Molecular Weight: 5,511 Da
• NCBI Official Full Name: gp130
• NCBI Official Synonym Full Names: interleukin 6 signal transducer (gp130, oncostatin M receptor)
• NCBI Official Symbol: IL6ST
• NCBI Official Synonym Symbols: CD130; GP130; CDW130; IL-6RB
• NCBI Protein Information: interleukin-6 receptor subunit beta; CD130 antigen; IL-6R subunit beta; membrane glycoprotein 130; IL-6 receptor subunit beta; membrane glycoprotein gp130; interleukin receptor beta chain; oncostatin-M receptor subunit alpha; gp130 of the rheumatoid arthritis antigenic peptide-bearing soluble form
• UniProt Protein Name: Gp130
• UniProt Gene Name: gp130
• UniProt Entry Name: Q13514_HUMAN

NCBI Description

The protein encoded by this gene is a signal transducer shared by many cytokines, including interleukin 6 (IL6), ciliary neurotrophic factor (CNTF), leukemia inhibitory factor (LIF), and oncostatin M (OSM). This protein functions as a part of the cytokine receptor complex. The activation of this protein is dependent upon the binding of cytokines to their receptors. vIL6, a protein related to IL6 and encoded by the Kaposi sarcoma-associated herpesvirus, can bypass the interleukin 6 receptor (IL6R) and directly activate this protein. Knockout studies in mice suggest that this gene plays a critical role in regulating myocyte apoptosis. Alternatively spliced transcript variants encoding distinct isoforms have been described. A related pseudogene has been identified on chromosome 17. [provided by RefSeq, Jul 2010]

Uniprot Description

gp130 iso7: gp130 is a ubiquitously expressed type I cytokine family receptor. The receptor systems for IL6, LIF, OSM, CNTF, IL11 AND CT1 utilize gp130 for initiating signal transmission. Binds to IL6/IL6R (alpha chain) complex, resulting in the formation of high-affinity IL6 binding sites, and transduces the signal. Does not directly bind IL6. May have a role in embryonic development. Contains 5 fibronectin type III domains and 1 immunoglobulin-like C2-type domain. Two alternatively spliced isoforms have been described.

Protein type: Receptor, cytokine; Membrane protein, integral

Chromosomal Location of Human Ortholog: 5q11.2

Cellular Component: extracellular space; membrane; cell soma; oncostatin-M receptor complex; dendrite; extracellular region; plasma membrane; interleukin-6 receptor complex; external side of plasma membrane

Molecular Function: interleukin-11 binding; protein binding; interleukin-27 receptor activity; interleukin-6 receptor binding; interleukin-6 binding; protein homodimerization activity; oncostatin-M receptor activity; leukemia inhibitory factor receptor activity; growth factor binding; ciliary neurotrophic factor receptor activity; interleukin-6 receptor activity; ciliary neurotrophic factor receptor binding; interleukin-11 receptor activity

Biological Process: glycogen metabolic process; viral reproduction; cytokine and chemokine mediated signaling pathway; leukemia inhibitory factor signaling pathway; positive regulation of tyrosine phosphorylation of Stat3 protein; positive regulation of osteoblast differentiation; positive regulation of tyrosine phosphorylation of Stat1 protein; regulation of Notch signaling pathway; positive regulation of acute inflammatory response; response to cytokine stimulus; positive regulation of cell proliferation; positive regulation of T cell proliferation; positive regulation of adaptive immune response; positive regulation of astrocyte differentiation; negative regulation of apoptosis