Glutamic-Oxaloacetic Transaminase 2 Recombinant Protein | GOT2 recombinant protein

Recombinant Human Glutamic-Oxaloacetic Transaminase 2

Cat. Num. AAA146276

• Gene Names: GOT2; KAT4; KATIV; mitAAT
• Purity: Greater than 95.0% as determined by SDS-PAGE.
• Synonyms: Glutamic-Oxaloacetic Transaminase 2; Recombinant Human Glutamic-Oxaloacetic Transaminase 2; GOT2 Human; Glutamic-Oxaloacetic Transaminase 2 Human Recombinant; EC 2.6.1.1; Aspartate aminotransferase 1; Transaminase A; GIG18; GOT2 recombinant protein

• Host: E Coli
• Purity/Purification: Greater than 95.0% as determined by SDS-PAGE.
• Form/Format: The GOT2 solution (0.5mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 0.15M NaCl, 10% glycerol and 1mM DTT.; Sterile filtered colorless solution.
• Sequence: MGSSHHHHHH SSGLVPRGSH MGSSSWWTHV EMGPPDPILG VTEAFKRDTN SKKMNLGVGA YRDDNGKPYV LPSVRKAEAQ IAAKNLDKEY LPIGGLAEFC KASAELALGE NSEVLKSGRF VTVQTISGTG ALRIGASFLQ RFFKFSRDVF LPKPTWGNHT PIFRDAGMQL QGYRYYDPKT CGFDFTGAVE DISKIPEQSV LLLHACAHNP TGVDPRPEQW KEIATVVKKR NLFAFFDMAY QGFASGDGDK DAWAVRHFIE QGINVCLCQS YAKNMGLYGE RVGAFTMVCK DADEAKRVES QLKILIRPMY SNPPLNGARI AAAILNTPDL RKQWLQEVKV MADRIIGMRT QLVSNLKKEG STHNWQHITD QIGMFCFTGL KPEQVERLIK EFSIYMTKDG RISVAGVTSS NVGYLAHAIH QVTK
• Sequence Length: 387

• Preparation and Storage: Store at 4 degree C if entire vial will be used within 2-4 weeks. Store, frozen at -20 degree C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.

Related Product Information for GOT2 recombinant protein

Description: GOT2 Recombinant Human produced in E Coli is a single, non-glycosylated polypeptide chain containing 424 amino acids (30-430 a.a.) and having a molecular mass of 47kDa. The GOT2 fused to a 23 amino acid his tag at N-terminus and is purified by proprietary chromatographic techniques.

Introduction: GOT2 is a pyridoxal phosphate-dependent enzyme which is found in cytoplasmic and inner-membrane mitochondrial forms, GOT1 and GOT2. GOT2 is invloved in amino acid metabolism and the urea and tricarboxylic acid cycles. The 2 enzymes are homodimeric and demonstrate close homology.

Product Categories/Family for GOT2 recombinant protein

Enzymes; Transaminase

NCBI and Uniprot Product Information

• NCBI GI #: 555290167
• NCBI GeneID: 2806
• NCBI Accession #: NP_001273149.1
• NCBI GenBank Nucleotide #: NM_001286220.1
• UniProt Accession #: P00505; Q53FL3; Q9BWA3; B4DJA6; E7ERW2
• Molecular Weight: 43,030 Da
• NCBI Official Full Name: aspartate aminotransferase, mitochondrial isoform 2
• NCBI Official Synonym Full Names: glutamic-oxaloacetic transaminase 2, mitochondrial
• NCBI Official Symbol: GOT2
• NCBI Official Synonym Symbols: KAT4; KATIV; mitAAT
• NCBI Protein Information: aspartate aminotransferase, mitochondrial; FABP-1; FABPpm; aspartate aminotransferase 2; aspartate transaminase 2; fatty acid-binding protein; glutamate oxaloacetate transaminase 2; glutamic-oxaloacetic transaminase 2, mitochondrial (aspartate aminotransferase 2); kynurenine aminotransferase 4; kynurenine aminotransferase IV; kynurenine--oxoglutarate transaminase 4; kynurenine--oxoglutarate transaminase IV; mAspAT; plasma membrane-associated fatty acid-binding protein; transaminase A
• UniProt Protein Name: Aspartate aminotransferase, mitochondrial
• UniProt Gene Name: GOT2
• UniProt Synonym Gene Names: mAspAT; FABP-1; FABPpm
• UniProt Entry Name: AATM_HUMAN

NCBI Description

Glutamic-oxaloacetic transaminase is a pyridoxal phosphate-dependent enzyme which exists in cytoplasmic and inner-membrane mitochondrial forms, GOT1 and GOT2, respectively. GOT plays a role in amino acid metabolism and the urea and tricarboxylic acid cycles. The two enzymes are homodimeric and show close homology. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2013]

Uniprot Description

GOT2: Catalyzes the irreversible transamination of the L- tryptophan metabolite L-kynurenine to form kynurenic acid (KA). Plays a key role in amino acid metabolism. Important for metabolite exchange between mitochondria and cytosol. Facilitates cellular uptake of long-chain free fatty acids. Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family.

Protein type: Amino Acid Metabolism - alanine, aspartate and glutamate; Transferase; EC 2.6.1.1; Amino Acid Metabolism - tyrosine; Amino Acid Metabolism - phenylalanine, tyrosine and tryptophan biosynthesis; Amino Acid Metabolism - arginine and proline; Amino Acid Metabolism - cysteine and methionine; EC 2.6.1.7; Mitochondrial; Amino Acid Metabolism - phenylalanine

Chromosomal Location of Human Ortholog: 16q21

Cellular Component: mitochondrion; mitochondrial matrix; mitochondrial inner membrane; plasma membrane

Molecular Function: kynurenine-oxoglutarate transaminase activity; aspartate transaminase activity; pyridoxal phosphate binding

Biological Process: glutamate metabolic process; oxaloacetate metabolic process; glutamate catabolic process to aspartate; glucose metabolic process; aspartate biosynthetic process; pathogenesis; gluconeogenesis; 2-oxoglutarate metabolic process; response to ethanol; 4-hydroxyproline catabolic process; carbohydrate metabolic process; aspartate catabolic process; aspartate metabolic process; amino acid biosynthetic process; glutamate catabolic process to 2-oxoglutarate; fatty acid transport