Horse Aspartate aminotransferase ELISA Kit | GOT2 elisa kit
Horse Aspartate aminotransferase, mitochondrial (GOT2) ELISA Kit
Gene Names
GOT2; FABP-1; FABPpm; mAspAT
Reactivity
Horse
Synonyms
Aspartate aminotransferase; Horse Aspartate aminotransferase; mitochondrial (GOT2) ELISA Kit; GOT2 elisa kit
Reactivity
Horse
Specificity
This assay has high sensitivity and excellent specificity for detection of Horse GOT2. No significant cross-reactivity or interference between Horse GOT2 and analogues was observed.
Sequence Length
448
Samples
Serum, plasma and other biological fluids
Assay Type
Quantitative Sandwich
Detection Range
15.6 ng/mL - 1000 ng/mL
Sensitivity
10.4 ng/mL (mean of 6 independent assays)
Intra-assay Precision
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision. Intra-Assay: CV<8%
Inter-assay Precision
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested in forty separate assays to assess inter-assay precision. CV (%) = SD/meanX100. Inter-Assay: CV<12%
Preparation and Storage
Store at 2-8 degree C.
Related Product Information for GOT2 elisa kit
Intended Uses: For the quantitative detection of Horse Aspartate aminotransferase, mitochondrial (GOT2) concentration in serum, plasma and other biological fluids.
Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate GOT2 in Horse serum, plasma. An antibody specific for GOT2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any GOT2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for GOT2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of GOT2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate GOT2 in Horse serum, plasma. An antibody specific for GOT2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any GOT2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for GOT2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of GOT2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
44,567 Da
NCBI Official Full Name
Aspartate aminotransferase, mitochondrial
NCBI Official Symbol
GOT2
NCBI Official Synonym Symbols
FABP-1; FABPpm; mAspAT
NCBI Protein Information
aspartate aminotransferase, mitochondrial
UniProt Protein Name
Aspartate aminotransferase, mitochondrial
Protein Family
UniProt Gene Name
GOT2
UniProt Synonym Gene Names
mAspAT; FABP-1; FABPpm
Uniprot Description
Catalyzes the irreversible transamination of the L-tryptophan metabolite L-kynurenine to form kynurenic acid (KA). Plays a key role in amino acid metabolism. Important for metabolite exchange between mitochondria and cytosol. Facilitates cellular uptake of long-chain free fatty acids ().
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Product Notes
The Horse GOT2 got2 (Catalog #AAA281270) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA281270 ELISA Kit recognizes Horse GOT2. It is sometimes possible for the material contained within the vial of "Aspartate aminotransferase, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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