Human glutamic-oxaloacetic transaminase 2, mitochondrial (aspartate aminotransferase 2) ELISA Kit | GOT2 elisa kit

Human glutamic-oxaloacetic transaminase 2, mitochondrial (aspartate aminotransferase 2) , GOT2 ELISA Kit

Cat. Num. AAA941094

• Gene Names: GOT2; KAT4; KATIV; mitAAT
• Reactivity: Human
• Synonyms: glutamic-oxaloacetic transaminase 2; mitochondrial (aspartate aminotransferase 2); Human glutamic-oxaloacetic transaminase 2; GOT2 ELISA Kit; mitochondrial (aspartate aminotransferase 2) (GOT2) ELISA kit; FLJ40994; KAT4; KATIV; mitAAT; aspartate aminotransferase 2; kynurenine aminotransferase IV; GOT2 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of human GOT2. No significant cross-reactivity or interference between human GOT2 and analogues was observed.
• Sequence Length: 387

• Samples: Serum, plasma, tissue homogenates
• Assay Type: Sandwich
• Detection Range: 0.156 mU/ml-10 mU/ml.
• Sensitivity: The minimum detectable dose of human GOT2 is typically less than 0.039 mU/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for GOT2 elisa kit

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for GOT2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any GOT2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for GOT2 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of GOT2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 555290167
• NCBI GeneID: 2806
• NCBI Accession #: NP_001273149.1
• NCBI GenBank Nucleotide #: NM_001286220.1
• UniProt Accession #: P00505; Q53FL3; Q9BWA3; B4DJA6; E7ERW2
• Molecular Weight: 47,518 Da
• NCBI Official Full Name: aspartate aminotransferase, mitochondrial isoform 2
• NCBI Official Synonym Full Names: glutamic-oxaloacetic transaminase 2, mitochondrial
• NCBI Official Symbol: GOT2
• NCBI Official Synonym Symbols: KAT4; KATIV; mitAAT
• NCBI Protein Information: aspartate aminotransferase, mitochondrial; FABP-1; FABPpm; mAspAT; transaminase A; aspartate transaminase 2; fatty acid-binding protein; aspartate aminotransferase 2; kynurenine aminotransferase 4; kynurenine aminotransferase IV; glutamate oxaloacetate transaminase 2; kynurenine--oxoglutarate transaminase 4; kynurenine--oxoglutarate transaminase IV; plasma membrane-associated fatty acid-binding protein; glutamic-oxaloacetic transaminase 2, mitochondrial (aspartate aminotransferase 2)
• UniProt Protein Name: Aspartate aminotransferase, mitochondrial
• UniProt Gene Name: GOT2
• UniProt Synonym Gene Names: mAspAT; FABP-1; FABPpm
• UniProt Entry Name: AATM_HUMAN

NCBI Description

Glutamic-oxaloacetic transaminase is a pyridoxal phosphate-dependent enzyme which exists in cytoplasmic and inner-membrane mitochondrial forms, GOT1 and GOT2, respectively. GOT plays a role in amino acid metabolism and the urea and tricarboxylic acid cycles. The two enzymes are homodimeric and show close homology. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2013]

Uniprot Description

GOT2: Catalyzes the irreversible transamination of the L- tryptophan metabolite L-kynurenine to form kynurenic acid (KA). Plays a key role in amino acid metabolism. Important for metabolite exchange between mitochondria and cytosol. Facilitates cellular uptake of long-chain free fatty acids. Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family.

Protein type: EC 2.6.1.1; Amino Acid Metabolism - tyrosine; Transferase; Amino Acid Metabolism - phenylalanine; Amino Acid Metabolism - arginine and proline; Mitochondrial; Amino Acid Metabolism - phenylalanine, tyrosine and tryptophan biosynthesis; Amino Acid Metabolism - alanine, aspartate and glutamate; Amino Acid Metabolism - cysteine and methionine; EC 2.6.1.7

Chromosomal Location of Human Ortholog: 16q21

Cellular Component: mitochondrion; mitochondrial matrix; mitochondrial inner membrane; plasma membrane

Molecular Function: kynurenine-oxoglutarate transaminase activity; aspartate transaminase activity; pyridoxal phosphate binding

Biological Process: glutamate metabolic process; oxaloacetate metabolic process; glutamate catabolic process to aspartate; aspartate biosynthetic process; glucose metabolic process; pathogenesis; 2-oxoglutarate metabolic process; gluconeogenesis; response to ethanol; 4-hydroxyproline catabolic process; carbohydrate metabolic process; aspartate catabolic process; aspartate metabolic process; glutamate catabolic process to 2-oxoglutarate; amino acid biosynthetic process; fatty acid transport