GOPC recombinant protein

GOPC, 278-454aa, Human, His tag, E Coli

Cat. Num. AAA203691

• Gene Names: GOPC; CAL; FIG; PIST; GOPC1; dJ94G16.2
• Applications: SDS-Page
• Purity: > 90% by SDS-PAGE
• Synonyms: GOPC; 278-454aa; Human; His tag; E Coli; Golgi-associated PDZ and coiled-coil motif-containing protein; CAL; dJ94G16.2; FIG; GOPC1; PIST; Golgi-associated PDZ and coiled-coil motif-containing protein CFTR associated ligand; FIG Fused in glioblastoma; Golgi associated PDZ and coiled coil motif containing; Golgi associated PDZ and coiled coil motif containing protein; GOPC 1; OTTHuMP00000040403; PDZ protein interacting specifically with TC 10; PDZ protein interacting specifically with TC10; PDZ/coiled coil domain binding partner for the rho family GTPase TC 10; PDZ/coiled coil domain binding partner for the rho family GTPase TC10; Protein interacting specifically with Tc 10; Protein interacting specifically with Tc10.; GOPC recombinant protein

• Host: E Coli
• Purity/Purification: > 90% by SDS-PAGE
• Form/Format: Liquid. In 20mM Tris-HCl Buffer (pH 8.0) containing 10% glycerol, 1mM DTT.
• Concentration: 1 mg/ml (determined by Bradford assay) (varies by lot)
• Sequence: MGSSHHHHHH SSGLVPRGSH MIRKVLLLKE DHEGLGISIT GGKEHGVPIL ISEIHPGQPA DRCGGLHVGD AILAVNGVNL RDTKHKEAVT ILSQQRGEIE FEVVYVAPEV DSDDENVEYE DESGHRYRLY LDELEGGGNP GASCKDTSGE IKVLQGFNKK AVTDTHENGD LGTASETPLD DGASKLDDLH TLYHKKSY
• Sequence Length: 454

• Applicable Applications for GOPC recombinant protein: SDS-PAGE
• Antigen Species: Human
• Tag Information: His-tag
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for GOPC recombinant protein

GOPC, also known as PIST, is a PDZ domain-containing Golgi protein. This protein functions as a homooligomer that interacts with a variety of proteins and plays a role in intracellular protein trafficking and degradation. Additionally, GOPC is thought to regulate ionic currents via membrane channel modification and may also play a role in autophagy. Chromosomal aberrations in the gene encoding GOPC are found in glioblastoma multiform (GBM), a common and aggressive form of brain tumor, suggesting a role for mutated PIST in carcinogenesis. Recombinant GOPC protein was expressed in E Coli and purified by using conventional chromatography techniques.

Product Categories/Family for GOPC recombinant protein

Adhesion & Movement

References

Cheng J., et al. (2002) J Biol Chem. 277 (5):3520-9. ; Yao R., et al. (2001) Biochem Biophys Res Commun. 286 (4):771-8.;

NCBI and Uniprot Product Information

• NCBI GI #: 62868213
• NCBI GeneID: 57120
• NCBI Accession #: NP_001017408
• NCBI GenBank Nucleotide #: NM_001017408.2
• UniProt Accession #: Q9HD26; Q59FS4; Q969U8; A6NM30
• Molecular Weight: 21.5 kDa (198aa), confirmed by MALDI-TOF. (Molecular weight on SDS-PAGE will appear higher)
• NCBI Official Full Name: Golgi-associated PDZ and coiled-coil motif-containing protein isoform b
• NCBI Official Synonym Full Names: golgi associated PDZ and coiled-coil motif containing
• NCBI Official Symbol: GOPC
• NCBI Official Synonym Symbols: CAL; FIG; PIST; GOPC1; dJ94G16.2
• NCBI Protein Information: Golgi-associated PDZ and coiled-coil motif-containing protein
• UniProt Protein Name: Golgi-associated PDZ and coiled-coil motif-containing protein
• Protein Family: Golgi-associated PDZ and coiled-coil motif-containing protein
• UniProt Gene Name: GOPC
• UniProt Synonym Gene Names: CAL; FIG; PIST

NCBI Description

This gene encodes a Golgi protein with a PDZ domain. The PDZ domain is globular and proteins which contain them bind other proteins through short motifs near the C-termini. Mice which are deficient in the orthologous protein have globozoospermia and are infertile. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2011]

Uniprot Description

Plays a role in intracellular protein trafficking and degradation. May regulate CFTR chloride currents and acid-induced ASIC3 currents by modulating cell surface expression of both channels. May also regulate the intracellular trafficking of the ADR1B receptor. May play a role in autophagy. Overexpression results in CFTR intracellular retention and degradation in the lysosomes.