Goat Anti-Mouse IgG2c, Human ads Secondary Antibody | IgG2c secondary antibody
Goat Anti-Mouse IgG2c, Human ads-PE
Applications
ELISA, Flow Cytometry, Functional Assay, ELISA, Immunohistochemistry, Immunoblot, Western Blot
Purity
Affinity chromatography on mouse IgG2c covalently linked to agarose
Synonyms
Goat Anti-Mouse IgG2c; Human ads; Human ads-PE; IgG2c secondary antibody
Host
Goat
Isotype
IgG
Specificity
Reacts with the heavy chain of C57BL/6 mouse IgG2c
Purity/Purification
Affinity chromatography on mouse IgG2c covalently linked to agarose
Concentration
0.5 mg/mL (varies by lot)
Applicable Applications for IgG2c secondary antibody
ELISA (EIA), FLISA, Flow Cytometry (FC/FACS), ELISpot (EIA), Immunohistochemistry (IHC) Paraffin, Immunoblot (IB), Depletion
Application Notes
Quality tested applications for relevant formats include - ELISA; FLISA; Other referenced applications for relevant formats include -; Flow Cytometry; ELISpot; Immunohistochemistry-Paraffin Sections; Immunoblotting; Depletion
Cross Adsorption
Mouse IgG1, IgG2a, IgG2b, IgG3, IgM, and IgA; human immunoglobulins and pooled sera; may react with immunoglobulins from other species
Buffer Formulation
Supplied in PBS/NaN3 and a stabilizing agent
Isotype Control
Goat IgG-PE
Preparation and Storage
Store at 2-8 degree C
References
1. Lambert SL, Okada CY, Levy R. TCR vaccines against a murine T cell lymphoma: a primary role for antibodies of the IgG2c class in tumor protection. J Immunol. 2004;172:929-36. (ELISA, Depletion)
2. Zhang H, High KA, Wu Q, Yang P, Schlachterman A, Yu S, et al. Genetic analysis of the antibody response to AAV2 and factor IX. Mol Ther. 2005;11:866-74. (ELISA)
3. Magalhaes JG, Fritz JH, Le Bourhis L, Sellge G, Travassos LH, Selvanantham T, et al. Nod2-dependent Th2 polarization of antigen-specific immunity. J Immunol. 2008;181:7925-35. (ELISA)
4. Burrell BE, Lu G, Li XC, Bishop DK. OX40 costimulation prevents allograft acceptance induced by CD40-CD40L blockade. J Immunol. 2009;182:379-90. (ELISA)
5. Swanson CL, Wilson TJ, Strauch P, Colonna M, Pelanda R, Torres RM. Type I IFN enhances follicular B cell contribution to the T cell-independent antibody response. J Exp Med. 2010;207:1485-1500. (ELISA, ELISPOT)
6. Kasturi SP, Skountzou I, Albrecht RA, Koutsonanos D, Hua T, Nakaya HI, et at. Programming the magnitude and persistence of antibody responses with innate immunity. Nature. 2011;470:543-7. (ELISA)
7. Schmitz JM, Durham CG, Schoeb TR, Soltau TD, Wolf KJ, Tanner SM, et al. Helicobacter felis-associated gastric disease in microbiota-restricted mice. J Histochem Cytochem. 2011;59:826-41. (ELISA)
8. Allan RS, Zueva E, Cammas F, Schreiber HA, Masson V, Belz GT, et al. An epigenetic silencing pathway controlling T helper 2 cell lineage commitment. Nature. 2012;487:249-53. (ELISA)
9. Harada Y, Tanaka S, Motomura Y, Harada Y, Ohno S, Ohno S, et al. The 3' enhancer CNS2 is a critical regulator of interleukin-4-mediated humoral immunity in follicular helper T cells. Immunity. 2012;36:188-200. (ELISA)
10. Vogelzang A, McGuire HM, Liu SM, Gloss B, Mercado K, Earls P, et al. IL-21 contributes to fatal inflammatory disease in the absence of Foxp3+ T regulatory cells. J Immunol. 2014;192:1404-14. (ELISA)
11. Halemano K, Guo K, Heilman KJ, Barrett BS, Smith DS, Hasenkrug KJ, et al. Immunoglobulin somatic hypermutation by APOBEC3/Rfv3 during retroviral infection. Proc Natl Acad Sci USA. 2014;111:7759-64. (ELISA)
12. Lam RS, O'Brien-Simpson NM, Lenzo JC, Holden JA, Brammar GC, Walsh KA, et al. Macrophage depletion abates Porphyromonas gingivalis-induced alveolar bone resorption in mice. J Immunol. 2014;193:2349-62. (ELISA)
13. Shen P, Roch T, Lampropoulou V, O'Connor RA, Stervbo U, Hilgenberg E, et al. IL-35-producing B cells are critical regulators of immunity during autoimmune and infectious diseases. Nature. 2014;507:366-70. (ELISA)
14. Soldemo M, Pedersen GK, Karlsson Hedestam GB. HIV-1 Env-specific memory and germinal center B cells in C57BL/6 mice. Viruses. 2014;6:3400-14. (ELISA)
15. Blaauboer SM, Gabrielle VD, Jin L. MPYS/STING-mediated TNF-alpha, not type I IFN, is essential for the mucosal adjuvant activity of (3'-5')-cyclic-di-guanosine-monophosphate in vivo. J Immunol. 2014;192:492-502. (ELISA)
16. Rowland SL, Riggs JM, Gilfillan S, Bugatti M, Vermi W, Kolbeck R, et al. Early, transient depletion of plasmacytoid dendritic cells ameliorates autoimmunity in a lupus model. J Exp Med. 2014;211:1977-91. (ELISA)
17. Lopez-Saucedo C, Bernal-Reynaga R, Zayas-Jahuey J, Galindo-Gomez S, Shibayama M, Garcia-Galvez C, et al. CD40 ligand deficient C57BL/6 mouse is a potential surrogate model of human X-linked hyper IgM (X-HIGM) syndrome for characterizing immune responses against pathogens. Biomed Res Int. 2015;2015:679850. (ELISA)
18. Teo T, Her Z, Tan JJ, Lum F, Lee WW, Chan Y, et al. Caribbean and La Réunion chikungunya virus isolates differ in their capacity to induce proinflammatory Th1 and NK cell responses and acute joint pathology. J Virol. 2015;89:7955-69. (ELISA)
19. Devera TS, Prusator DK, Joshi SK, Ballard JD, Lang ML. Immunization of mice with anthrax protective antigen limits cardiotoxicity but not hepatotoxicity following lethal toxin challenge. Toxins. 2015;7:2371-84. (ELISA)
20. Blaauboer SM, Mansouri S, Tucker HR, Wang HL, Gabrielle VD, Jin L. The mucosal adjuvant cyclic di-GMP enhances antigen uptake and selectively activates pinocytosis-efficient cells in vivo. Elife. 2015;4:e06670. (ELISA)
21. Bai L, Deng S, Reboulet R, Mathew R, Teyton L, Savage PB, et al. Natural killer T (NKT)-B-cell interactions promote prolonged antibody responses and long-term memory to pneumococcal capsular polysaccharides. Proc Natl Acad Sci USA. 2013;110:16097-102. (FC)
22. Limon JJ, So L, Jellbauer S, Chiu H, Corado J, Sykes SM, et al. mTOR kinase inhibitors promote antibody class switching via mTORC2 inhibition. Proc Natl Acad Sci USA. 2014;111:E5076-85. (FC)
23. Hsu H, Wu Y, Yang P, Wu Q, Job G, Chen J, et al. Overexpression of activation-induced cytidine deaminase in B cells is associated with production of highly pathogenic autoantibodies. J Immunol. 2007;178:5357-65. (ELISPOT)
24. Betz BC, Jordan-Williams KL, Wang C, Kang SG, Liao J, Logan MR, et al. Batf coordinates multiple aspects of B and T cell function required for normal antibody responses. J Exp Med. 2010;207:933-42. (IHC-PS)
25. Esser-von Bieren J, Volpe B, Kulagin M, Sutherland DB, Guiet R, Seitz A, et al. Antibody-mediated trapping of helminth larvae requires CD11b and Fcgamma receptor I. J Immunol. 2015;194:1154-63. (FC, WB, Depletion)
2. Zhang H, High KA, Wu Q, Yang P, Schlachterman A, Yu S, et al. Genetic analysis of the antibody response to AAV2 and factor IX. Mol Ther. 2005;11:866-74. (ELISA)
3. Magalhaes JG, Fritz JH, Le Bourhis L, Sellge G, Travassos LH, Selvanantham T, et al. Nod2-dependent Th2 polarization of antigen-specific immunity. J Immunol. 2008;181:7925-35. (ELISA)
4. Burrell BE, Lu G, Li XC, Bishop DK. OX40 costimulation prevents allograft acceptance induced by CD40-CD40L blockade. J Immunol. 2009;182:379-90. (ELISA)
5. Swanson CL, Wilson TJ, Strauch P, Colonna M, Pelanda R, Torres RM. Type I IFN enhances follicular B cell contribution to the T cell-independent antibody response. J Exp Med. 2010;207:1485-1500. (ELISA, ELISPOT)
6. Kasturi SP, Skountzou I, Albrecht RA, Koutsonanos D, Hua T, Nakaya HI, et at. Programming the magnitude and persistence of antibody responses with innate immunity. Nature. 2011;470:543-7. (ELISA)
7. Schmitz JM, Durham CG, Schoeb TR, Soltau TD, Wolf KJ, Tanner SM, et al. Helicobacter felis-associated gastric disease in microbiota-restricted mice. J Histochem Cytochem. 2011;59:826-41. (ELISA)
8. Allan RS, Zueva E, Cammas F, Schreiber HA, Masson V, Belz GT, et al. An epigenetic silencing pathway controlling T helper 2 cell lineage commitment. Nature. 2012;487:249-53. (ELISA)
9. Harada Y, Tanaka S, Motomura Y, Harada Y, Ohno S, Ohno S, et al. The 3' enhancer CNS2 is a critical regulator of interleukin-4-mediated humoral immunity in follicular helper T cells. Immunity. 2012;36:188-200. (ELISA)
10. Vogelzang A, McGuire HM, Liu SM, Gloss B, Mercado K, Earls P, et al. IL-21 contributes to fatal inflammatory disease in the absence of Foxp3+ T regulatory cells. J Immunol. 2014;192:1404-14. (ELISA)
11. Halemano K, Guo K, Heilman KJ, Barrett BS, Smith DS, Hasenkrug KJ, et al. Immunoglobulin somatic hypermutation by APOBEC3/Rfv3 during retroviral infection. Proc Natl Acad Sci USA. 2014;111:7759-64. (ELISA)
12. Lam RS, O'Brien-Simpson NM, Lenzo JC, Holden JA, Brammar GC, Walsh KA, et al. Macrophage depletion abates Porphyromonas gingivalis-induced alveolar bone resorption in mice. J Immunol. 2014;193:2349-62. (ELISA)
13. Shen P, Roch T, Lampropoulou V, O'Connor RA, Stervbo U, Hilgenberg E, et al. IL-35-producing B cells are critical regulators of immunity during autoimmune and infectious diseases. Nature. 2014;507:366-70. (ELISA)
14. Soldemo M, Pedersen GK, Karlsson Hedestam GB. HIV-1 Env-specific memory and germinal center B cells in C57BL/6 mice. Viruses. 2014;6:3400-14. (ELISA)
15. Blaauboer SM, Gabrielle VD, Jin L. MPYS/STING-mediated TNF-alpha, not type I IFN, is essential for the mucosal adjuvant activity of (3'-5')-cyclic-di-guanosine-monophosphate in vivo. J Immunol. 2014;192:492-502. (ELISA)
16. Rowland SL, Riggs JM, Gilfillan S, Bugatti M, Vermi W, Kolbeck R, et al. Early, transient depletion of plasmacytoid dendritic cells ameliorates autoimmunity in a lupus model. J Exp Med. 2014;211:1977-91. (ELISA)
17. Lopez-Saucedo C, Bernal-Reynaga R, Zayas-Jahuey J, Galindo-Gomez S, Shibayama M, Garcia-Galvez C, et al. CD40 ligand deficient C57BL/6 mouse is a potential surrogate model of human X-linked hyper IgM (X-HIGM) syndrome for characterizing immune responses against pathogens. Biomed Res Int. 2015;2015:679850. (ELISA)
18. Teo T, Her Z, Tan JJ, Lum F, Lee WW, Chan Y, et al. Caribbean and La Réunion chikungunya virus isolates differ in their capacity to induce proinflammatory Th1 and NK cell responses and acute joint pathology. J Virol. 2015;89:7955-69. (ELISA)
19. Devera TS, Prusator DK, Joshi SK, Ballard JD, Lang ML. Immunization of mice with anthrax protective antigen limits cardiotoxicity but not hepatotoxicity following lethal toxin challenge. Toxins. 2015;7:2371-84. (ELISA)
20. Blaauboer SM, Mansouri S, Tucker HR, Wang HL, Gabrielle VD, Jin L. The mucosal adjuvant cyclic di-GMP enhances antigen uptake and selectively activates pinocytosis-efficient cells in vivo. Elife. 2015;4:e06670. (ELISA)
21. Bai L, Deng S, Reboulet R, Mathew R, Teyton L, Savage PB, et al. Natural killer T (NKT)-B-cell interactions promote prolonged antibody responses and long-term memory to pneumococcal capsular polysaccharides. Proc Natl Acad Sci USA. 2013;110:16097-102. (FC)
22. Limon JJ, So L, Jellbauer S, Chiu H, Corado J, Sykes SM, et al. mTOR kinase inhibitors promote antibody class switching via mTORC2 inhibition. Proc Natl Acad Sci USA. 2014;111:E5076-85. (FC)
23. Hsu H, Wu Y, Yang P, Wu Q, Job G, Chen J, et al. Overexpression of activation-induced cytidine deaminase in B cells is associated with production of highly pathogenic autoantibodies. J Immunol. 2007;178:5357-65. (ELISPOT)
24. Betz BC, Jordan-Williams KL, Wang C, Kang SG, Liao J, Logan MR, et al. Batf coordinates multiple aspects of B and T cell function required for normal antibody responses. J Exp Med. 2010;207:933-42. (IHC-PS)
25. Esser-von Bieren J, Volpe B, Kulagin M, Sutherland DB, Guiet R, Seitz A, et al. Antibody-mediated trapping of helminth larvae requires CD11b and Fcgamma receptor I. J Immunol. 2015;194:1154-63. (FC, WB, Depletion)
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Product Notes
The IgG2c (Catalog #AAA670664) is a Secondary Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Goat Anti-Mouse IgG2c, Human ads can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), FLISA, Flow Cytometry (FC/FACS), ELISpot (EIA), Immunohistochemistry (IHC) Paraffin, Immunoblot (IB), Depletion. Quality tested applications for relevant formats include - ELISA; FLISA; Other referenced applications for relevant formats include -; Flow Cytometry; ELISpot; Immunohistochemistry-Paraffin Sections; Immunoblotting; Depletion. Researchers should empirically determine the suitability of the IgG2c for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Goat Anti-Mouse IgG2c, Human ads, Secondary Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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