Dot Blot
(Dot Blot of Goat anti-Rabbit IgG Fc Antibody (Min X Human) Alkaline Phosphatase Conjugated. Antigen: Rabbit IgG Fc. Load: Lane 1 - 200 ng Lane 2 - 66.67 ng Lane 3 - 22.22 ng Lane 4 - 7.41 ng Lane 5 - 2.47 ng. Primary antibody: none. Secondary antibody: Goat anti-Rabbit IgG Fc Antibody (Min X Human) Alkaline Phosphatase Conjugated at 1:1,000 for 60 min at RT. Block: MB-070 for 60 min at RT. Visualized using NBT-100 Alkaline Phosphatase Substrate for 30 seconds at RT.)
Goat anti Rabbit IgG (Alk Phos) Secondary Antibody | IgG secondary antibody
Goat anti Rabbit IgG (Alk Phos)
Reactivity
Rabbit IgG
Applications
ELISA, Immunohistochemistry, Western Blot
Purity
Goat anti Rabbit IgG (Alk Phos) was purified by affinity chromatography.
Synonyms
Goat anti Rabbit IgG (Alk Phos); Goat anti Rabbit Immunoglobulin G secondary (Alk Phos); IgG secondary antibody
Host
Goat
Reactivity
Rabbit IgG
Specificity
Rabbit IgG
Purity/Purification
Goat anti Rabbit IgG (Alk Phos) was purified by affinity chromatography.
Form/Format
0.05M TRIS-HCl, pH 8.0, with 0.15M NaCl, 0.001M MgCl, 0.0001M ZnCl, 50% glycerol, 10 mg/ml BSA, and 0.01% NaN3. Immunoglobulin and protease free.
Concentration
1.0 mg/ml (varies by lot)
Applicable Applications for IgG secondary antibody
ELISA (EIA), Immunohistochemistry (IHC), Western Blot (WB)
Application Notes
ELISA: 1:2,000-1:10,000
IHC: 1:200-1:1,000
WB: 1:500-1:2,500
IHC: 1:200-1:1,000
WB: 1:500-1:2,500
Biohazard Information
This product contains sodium azide as preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.
Tag/Conjugate
Alkaline Phosphatase
Immunogen
Goat anti-rabbit IgG (Alk Phos) was raised in goat using rabbit IgG F(c) fragment as the immunogen.
Preparation and Storage
Store at 4 degree C. DO NOT FREEZE.
Dot Blot
(Dot Blot of Goat anti-Rabbit IgG Fc Antibody (Min X Human) Alkaline Phosphatase Conjugated. Antigen: Rabbit IgG Fc. Load: Lane 1 - 200 ng Lane 2 - 66.67 ng Lane 3 - 22.22 ng Lane 4 - 7.41 ng Lane 5 - 2.47 ng. Primary antibody: none. Secondary antibody: Goat anti-Rabbit IgG Fc Antibody (Min X Human) Alkaline Phosphatase Conjugated at 1:1,000 for 60 min at RT. Block: MB-070 for 60 min at RT. Visualized using NBT-100 Alkaline Phosphatase Substrate for 30 seconds at RT.)
Dot Blot
(Dot Blot of Goat anti-Rabbit IgG Fc Antibody (Min X Human) Alkaline Phosphatase Conjugated. Antigen: Rabbit IgG Fc. Load: Lane 1 - 200 ng Lane 2 - 66.67 ng Lane 3 - 22.22 ng Lane 4 - 7.41 ng Lane 5 - 2.47 ng. Primary antibody: none. Secondary antibody: Goat anti-Rabbit IgG Fc Antibody (Min X Human) Alkaline Phosphatase Conjugated at 1:1,000 for 60 min at RT. Block: MB-070 for 60 min at RT. Visualized using NBT-100 Alkaline Phosphatase Substrate for 30 seconds at RT.)
Related Product Information for IgG secondary antibody
Goat anti Rabbit IgG secondary antibody (Alk Phos)
Product Categories/Family for IgG secondary antibody
Similar Products
Product Notes
The IgG (Catalog #AAA538612) is a Secondary Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti Rabbit IgG (Alk Phos) reacts with Rabbit IgG and may cross-react with other species as described in the data sheet. AAA Biotech's Goat anti Rabbit IgG (Alk Phos) can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Immunohistochemistry (IHC), Western Blot (WB). ELISA: 1:2,000-1:10,000 IHC: 1:200-1:1,000 WB: 1:500-1:2,500. Researchers should empirically determine the suitability of the IgG for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Goat anti Rabbit IgG (Alk Phos), Secondary Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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