Goat Trem2 Antibody | anti-Trem2 antibody

Goat anti-Trem2 (mouse) Antibody

Cat. Num. AAA422239

• Gene Names: Trem2; TREM-2; Trem2a; Trem2b; Trem2c
• Reactivity: Tested: Mouse; Expected from sequence similarity: Mouse, Rat
• Applications: ELISA, Western Blot
• Purity: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Synonyms: Trem2; Goat anti-Trem2 (mouse) Antibody; triggering receptor expressed on myeloid cells 2; Trem2a; Trem2b; Trem2c; triggering receptor expressed on myeloid cells 2a; triggering receptor expressed on myeloid cells 2b; triggering receptor expressed on myeloid cells 2c; anti-Trem2 antibody

• Host: Goat
• Reactivity: Tested: Mouse; Expected from sequence similarity: Mouse, Rat
• Purity/Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Form/Format: Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
• Sequence: QVEHSTSRNQET
• Sequence Length: 227

• Applicable Applications for anti-Trem2 antibody: Peptide ELISA (EIA), Western Blot (WB)
• Application Notes: Peptide ELISA: antibody detection limit dilution 1:32000.; ; Western blot: Preliminary experiments showed a band at approx 26 kDa in Mouse and Rat Adrenal gland lysates after 1 ug/ml antibody staining (calculated MW of 24.5 kDa according to Mouse NP_112544.1 and 24.9 according to Rat NP_001100354.1). Primary incubation 1 hour at room temperature.; ; Immunofluorescence: Strong expression of the protein seen in the Plasma Membrane of NIH3T3-L1 (adipocyte;like) cells. Recommended concentration: 10 ug/ml.
• Immunogen: Peptide with sequence C-QVEHSTSRNQET, from the internal region of the protein sequence according to NP_112544.1.
• Preparation and Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Western Blot (WB)

((0.3ug/ml) staining of Mouse Liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

Immunofluorescence

(Immunofluorescence analysis of paraformaldehyde fixed 3T3-L1 cells, permeabilized with 0.15% Triton.Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane/cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).)

NCBI and Uniprot Product Information

• NCBI GI #: 13994125
• NCBI GeneID: 83433
• NCBI Accession #: NP_112544.1
• NCBI GenBank Nucleotide #: NM_031254.3
• NCBI Official Full Name: triggering receptor expressed on myeloid cells 2 isoform 1
• NCBI Official Synonym Full Names: triggering receptor expressed on myeloid cells 2
• NCBI Official Symbol: Trem2
• NCBI Official Synonym Symbols: TREM-2; Trem2a; Trem2b; Trem2c
• NCBI Protein Information: triggering receptor expressed on myeloid cells 2
• UniProt Protein Name: Triggering receptor expressed on myeloid cells 2
• Protein Family: Triggering receptor expressed on myeloid cells
• UniProt Gene Name: Trem2
• UniProt Synonym Gene Names: Trem2a; Trem2b; Trem2c; TREM-2
• UniProt Entry Name: TREM2_MOUSE

NCBI Description

The protein encoded by this gene is part of the immunoglobulin and lectin-like superfamily and functions as part of the innate immune system. This gene forms part of a cluster of genes on mouse chromosome 17 thought to be involved in innate immunity. This protein associates with the adaptor protein Dap-12 and recruits several factors, such as kinases and phospholipase C-gamma, to form a receptor signaling complex that activates myeloid cells, including dendritic cells and microglia. In humans homozygous loss-of-function mutations in this gene cause Nasu-Hakola disease and mutations in this gene may be risk factors to the development of Alzheimer's disease. In mouse mutations of this gene serve as a pathophysiological model for polycystic lipomembranous osteodysplasia with sclerosing leukoencephalopathy (Nasu-Hakola disease) and for inflammatory bowel disease. Alternative splicing results in multiple transcript variants that encode different protein isoforms. [provided by RefSeq, Jan 2013]

Uniprot Description

TREM2: May have a role in chronic inflammations and may stimulate production of constitutive rather than inflammatory chemokines and cytokines. Forms a receptor signaling complex with TYROBP and triggers activation of the immune responses in macrophages and dendritic cells. Defects in TREM2 are a cause of polycystic lipomembranous osteodysplasia with sclerosing leukoencephalopathy (PLOSL); also known as presenile dementia with bone cysts or Nasu-Hakola disease (NHD). PLOSL is a recessively inherited disease characterized by a combination of psychotic symptoms rapidly progressing to presenile dementia and bone cysts restricted to wrists and ankles. PLOSL has a global distribution, although most of the patients have been diagnosed in Finland and Japan, with an estimated population prevalence of 2x10(-6) in the Finns. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Cellular Component: extracellular region; integral to membrane; intracellular membrane-bound organelle; membrane; plasma membrane

Molecular Function: lipopolysaccharide binding; peptidoglycan binding; protein binding; transmembrane receptor activity

Biological Process: detection of lipopolysaccharide; detection of peptidoglycan; innate immune response; lipopolysaccharide-mediated signaling pathway; phagocytosis, engulfment; positive regulation of antigen processing and presentation of peptide antigen via MHC class II; positive regulation of calcium-mediated signaling; positive regulation of peptidyl-tyrosine phosphorylation