Rabbit anti-Human TUBA1C Polyclonal Antibody | anti-TUBA1C antibody

TUBA1C, CT (TUBA1C, TUBA6, Tubulin alpha-1C chain, Alpha-tubulin 6, Tubulin alpha-6 chain) (MaxLight 490)

Cat. Num. AAA6360107

• Gene Names: TUBA1C; TUBA6; bcm948
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Protein A and Peptide Affinity Chromatography.
• Synonyms: TUBA1C; Polyclonal Antibody; CT (TUBA1C; TUBA6; Tubulin alpha-1C chain; Alpha-tubulin 6; Tubulin alpha-6 chain) (MaxLight 490); Tubulin alpha-6 chain; anti-TUBA1C antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human TUBA1C. Species Crossreactivity: mouse and rat
• Purity/Purification: Purified by Protein A and Peptide Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight490.

• Applicable Applications for anti-TUBA1C antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:10-50; FLISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 414-441 in the C-terminal region of human TUBA1C, UniProt Accession #Q9BQE3, NP_116093.1. Species sequence homology: Xenopus
• Conjugate: MaxLight490
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight490 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis in K562 cell line lysates (35ug/lane) usingMBS6006298. This demonstrates MBS6006298 detected the TUBA1C protein (arrow).)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis of formalin fixed and paraffin embedded human lung tissue using MBS6006298 followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of MBS6006298 for immunohistochemistry.)

Related Product Information for anti-TUBA1C antibody

Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain.

Product Categories/Family for anti-TUBA1C antibody

Antibodies; Tubulin

References

Frum, R., et al. J. Proteome Res. 6(4):1410-1417(2007). Olsen, J.V., et al. Cell 127(3):635-648(2006). Olsen, J.V., et al. Cell 127(3):635-648(2006). Guo, D., et al. Biochem. Biophys. Res. Commun. 337(4):1308-1318(2005). Rush, J., et al. Nat. Biotechnol. 23(1):94-101(2005).

NCBI and Uniprot Product Information

• NCBI GI #: 142375723
• NCBI GeneID: 84790
• NCBI Accession #: NP_116093.1
• NCBI GenBank Nucleotide #: NM_032704.3
• UniProt Accession #: Q9BQE3
• Molecular Weight: 49,895 Da
• NCBI Official Full Name: Homo sapiens tubulin, alpha 1c (TUBA1C), mRNA
• NCBI Official Synonym Full Names: tubulin, alpha 1c
• NCBI Official Symbol: TUBA1C
• NCBI Official Synonym Symbols: TUBA6; bcm948
• NCBI Protein Information: tubulin alpha-1C chain; alpha-tubulin 6; tubulin alpha-6 chain; tubulin, alpha 6
• UniProt Protein Name: Tubulin alpha-1C chain
• Protein Family: Tubulin
• UniProt Gene Name: TUBA1C
• UniProt Synonym Gene Names: TUBA6
• UniProt Entry Name: TBA1C_HUMAN

Uniprot Description

Function: Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.

Subunit structure: Dimer of alpha and beta chains.

Subcellular location: Cytoplasm › cytoskeleton.

Post-translational modification: Undergoes a tyrosination/detyrosination cycle, the cyclic removal and re-addition of a C-terminal tyrosine residue by the enzymes tubulin tyrosine carboxypeptidase (TTCP) and tubulin tyrosine ligase (TTL), respectively

By similarity.Some glutamate residues at the C-terminus are polyglutamylated. This modification occurs exclusively on glutamate residues and results in polyglutamate chains on the gamma-carboxyl group. Also monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella) whereas glutamylation is prevalent in neuronal cells, centrioles, axonemes, and the mitotic spindle. Both modifications can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of such modifications is still unclear but they regulate the assembly and dynamics of axonemal microtubules

Probable.Acetylation of alpha chains at Lys-40 stabilizes microtubules and affects affinity and processivity of microtubule motors. This modification has a role in multiple cellular functions, ranging from cell motility, cell cycle progression or cell differentiation to intracellular trafficking and signaling

By similarity.

Sequence similarities: Belongs to the tubulin family.