Western Blot (WB)
(Western blot of Phycoerythrin conjugated Goat F(ab')2 Anti-Human IgG F(c). Lane 1: Human Fc. Lane 2: None. Load: 50 ng per lane. None. Phycoerythrin Goat secondary antibody at 1:1,000 for 60 min at RT. Blocking: Blocking buffer for 30 min at RT. Size: 28 kDa, 28 kDa for Human IgG F(c)..)
Goat anti Human IgG (Fab'2) (PE) Secondary Antibody | IgG secondary antibody
Goat anti Human IgG (Fab'2) (PE)
Reactivity
Human IgG (Fab2)
Applications
Flow Cytometry, Functional Assay, Immunofluorescence
Purity
Goat anti Human IgG (Fab'2) (PE) was purified by affinity chromatography.
Synonyms
Goat anti Human IgG (Fab'2) (PE); Goat anti Human Immunoglobulin G secondary (Fab'2) (PE); IgG secondary antibody
Host
Goat
Reactivity
Human IgG (Fab2)
Specificity
Human IgG (Fab2)
Purity/Purification
Goat anti Human IgG (Fab'2) (PE) was purified by affinity chromatography.
Form/Format
Supplied in liquid form in 0.02M K3PO4, pH 7.2, with 0.15M NaCl, 10 mg/ml BSA, and 0.05% NaN3. Immunoglobulin and protease free.
Applicable Applications for IgG secondary antibody
Flow Cytometry (FC/FACS), Immunofluorescence (IF)
Application Notes
FC: 1:100-1:250
IF: 1:100-1:250
IF: 1:100-1:250
Biological Significance
Immunoglobulin G (IgG) are antibody molecules. Each IgG is composed of four peptide chains - two heavy chains and two light chains. Each IgG has two antigen binding sites. Other Immunoglobulins may be described in terms of polymers with the IgG structure considered the monomer. The Fab region, (fragment, antigen binding), is the binding region of the antibody.
Biohazard Information
This product contains sodium azide as preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.
Tag/Conjugate
PE
Immunogen
Goat anti-human IgG (Fab'2) (PE) was raised in goat using human IgG F(c) fragment as the immunogen.
Preparation and Storage
Store at 4 degree C for short term storage. Aliquot and freeze at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.
Western Blot (WB)
(Western blot of Phycoerythrin conjugated Goat F(ab')2 Anti-Human IgG F(c). Lane 1: Human Fc. Lane 2: None. Load: 50 ng per lane. None. Phycoerythrin Goat secondary antibody at 1:1,000 for 60 min at RT. Blocking: Blocking buffer for 30 min at RT. Size: 28 kDa, 28 kDa for Human IgG F(c)..)
Western Blot (WB)
(Western blot of Phycoerythrin conjugated Goat F(ab')2 Anti-Human IgG F(c). Lane 1: Human Fc. Lane 2: None. Load: 50 ng per lane. None. Phycoerythrin Goat secondary antibody at 1:1,000 for 60 min at RT. Blocking: Blocking buffer for 30 min at RT. Size: 28 kDa, 28 kDa for Human IgG F(c)..)
Related Product Information for IgG secondary antibody
Goat anti Human IgG secondary antibody (Fab'2) (PE)
Product Categories/Family for IgG secondary antibody
Similar Products
Product Notes
The IgG (Catalog #AAA539083) is a Secondary Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti Human IgG (Fab'2) (PE) reacts with Human IgG (Fab2) and may cross-react with other species as described in the data sheet. AAA Biotech's Goat anti Human IgG (Fab'2) (PE) can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), Immunofluorescence (IF). FC: 1:100-1:250 IF: 1:100-1:250. Researchers should empirically determine the suitability of the IgG for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Goat anti Human IgG (Fab'2) (PE), Secondary Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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