Immunofluorescence (IF)
(Immunofluorescence analysis of paraformaldehyde fixed NIH3T3 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing a low level of nuclear staining. Actin filaments)
Goat HNF4A Antibody | anti-HNF4A antibody
Goat Anti-HNF4A Antibody
Gene Names
HNF4A; TCF; HNF4; MODY; FRTS4; MODY1; NR2A1; TCF14; HNF4a7; HNF4a8; HNF4a9; NR2A21; HNF4alpha
Reactivity
MouseExpected from sequence similarity: Human, Mouse, Rat, Dog
Applications
ELISA, Immunofluorescence
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Synonyms
HNF4A; Goat Anti-HNF4A Antibody; hepatocyte nuclear factor 4; alpha ; FLJ39654; HNF4; HNF4a7; HNF4a8; HNF4a9; MODY; MODY1; NR2A1; NR2A21; TCF; TCF14; HNF4-alpha; hepatic nuclear factor 4 alpha; hepatocyte nuclear factor 4 alpha; transcription factor-14; anti-HNF4A antibody
Host
Goat
Reactivity
Mouse
Expected from sequence similarity: Human, Mouse, Rat, Dog
Expected from sequence similarity: Human, Mouse, Rat, Dog
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Sequence Length
464
Applicable Applications for anti-HNF4A antibody
Peptide ELISA (EIA), Immunofluorescence (IF)
Application Notes
Peptide ELISA: antibody detection limit dilution 1:16000
WB: Preliminary experiments showed an approx 55kDa band in Human Colon, Duodenum, Kidney and Liver lysates (calculated MW of 52.8kDa according to NP_000448.3). Recommended concentration:0.3-1ug/ml. Primary incubation 1 hour at room temperature.
IF: Expression of the protein seen in the nucleus of NIH3T3 cells. Recommended concentration: 10ug/ml.
WB: Preliminary experiments showed an approx 55kDa band in Human Colon, Duodenum, Kidney and Liver lysates (calculated MW of 52.8kDa according to NP_000448.3). Recommended concentration:0.3-1ug/ml. Primary incubation 1 hour at room temperature.
IF: Expression of the protein seen in the nucleus of NIH3T3 cells. Recommended concentration: 10ug/ml.
Immunogen
Peptide with sequence C-HPHLMQEHMGTN, from the internal region of the protein sequence according to NP_849180.1; NP_000448.3; NP_787110.2; NP_001025174.1; NP_001245284.1; NP_001274111.1; NP_001274112.1.
Peptide Sequence
HPHLMQEHMGTN
Epitope
Internal region
Important comments
This antibody is expected to recognise isoforms 1, 2, 4, 5, 7, 8 and 9
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
Immunofluorescence (IF)
(Immunofluorescence analysis of paraformaldehyde fixed NIH3T3 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing a low level of nuclear staining. Actin filaments)
Immunofluorescence (IF)
(Immunofluorescence analysis of paraformaldehyde fixed NIH3T3 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing a low level of nuclear staining. Actin filaments)
References
Aguilar-Salinas CA, Reyes-Rodriguez E, Ordonez-Sanchez ML, Torres MA, Ramirez-Jimenez S, Dominguez-Lopez A, Martinez-Francois JR, Velasco-Perez ML, Alpizar M, Garcia-Garcia E, Gomez-Perez F, Rull J, Tusie-Luna MT.: Early-onset type 2 diabetes: metabolic and genetic characterization in the mexican population. J Clin Endocrinol Metab. 2001 Jan;86(1):220-6. PMID: 11232004
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
hepatocyte nuclear factor 4-alpha isoform 1
NCBI Official Synonym Full Names
hepatocyte nuclear factor 4 alpha
NCBI Official Symbol
HNF4A
NCBI Official Synonym Symbols
TCF; HNF4; MODY; FRTS4; MODY1; NR2A1; TCF14; HNF4a7; HNF4a8; HNF4a9; NR2A21; HNF4alpha
NCBI Protein Information
hepatocyte nuclear factor 4-alpha
Protein Family
NCBI Description
The protein encoded by this gene is a nuclear transcription factor which binds DNA as a homodimer. The encoded protein controls the expression of several genes, including hepatocyte nuclear factor 1 alpha, a transcription factor which regulates the expression of several hepatic genes. This gene may play a role in development of the liver, kidney, and intestines. Mutations in this gene have been associated with monogenic autosomal dominant non-insulin-dependent diabetes mellitus type I. Alternative splicing of this gene results in multiple transcript variants encoding several different isoforms. [provided by RefSeq, Apr 2012]
Research Articles on HNF4A
Similar Products
Product Notes
The HNF4A (Catalog #AAA423841) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat Anti-HNF4A Antibody reacts with Mouse Expected from sequence similarity: Human, Mouse, Rat, Dog and may cross-react with other species as described in the data sheet. AAA Biotech's HNF4A can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Immunofluorescence (IF). Peptide ELISA: antibody detection limit dilution 1:16000 WB: Preliminary experiments showed an approx 55kDa band in Human Colon, Duodenum, Kidney and Liver lysates (calculated MW of 52.8kDa according to NP_000448.3). Recommended concentration:0.3-1ug/ml. Primary incubation 1 hour at room temperature. IF: Expression of the protein seen in the nucleus of NIH3T3 cells. Recommended concentration: 10ug/ml. Researchers should empirically determine the suitability of the HNF4A for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HNF4A, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
