Rabbit anti-Human SPOP Polyclonal Antibody | anti-SPOP antibody

SPOP, NT (SPOP, Speckle-type POZ protein, HIB homolog 1, Roadkill homolog 1) (FITC)

Cat. Num. AAA6350732

• Gene Names: SPOP; TEF2; BTBD32; NEDMACE; NEDMIDF
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: SPOP; Polyclonal Antibody; NT (SPOP; Speckle-type POZ protein; HIB homolog 1; Roadkill homolog 1) (FITC); Roadkill homolog 1; anti-SPOP antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).
• Sequence Length: 374

• Applicable Applications for anti-SPOP antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: SPOP antibody is generated from rabbits immunized with a KLH conjugated synthetic between 85-115 amino acids selected from the N-terminal region of human SPOP.
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(SPOP Antibody (N-term) western blot analysis in WiDr cell line lysates (35ug/lane).This demonstrates the SPOP antibody detected the SPOP protein (arrow).)

Related Product Information for anti-SPOP antibody

This gene encodes a protein that may modulate the transcriptional repression activities of death-associated protein 6 (DAXX), which interacts with histone deacetylase, core histones, and other histone-associated proteins. In mouse, the encoded protein binds to the putative leucine zipper domain of macroH2A1.2, a variant H2A histone that is enriched on inactivated X chromosomes. The BTB/POZ domain of this protein has been shown in other proteins to mediate transcriptional repression and to interact with components of histone deacetylase co-repressor complexes. Alternative splicing of this gene results in multiple transcript variants encoding the same protein. [provided by RefSeq].

Product Categories/Family for anti-SPOP antibody

Antibodies; Transcription Factors

References

Rose, J. Phd, et al. Mol. Med. (2010). In press: Zhuang, M., et al. Mol. Cell 36(1):39-50(2009). Liu, J., et al. Science 323(5918):1218-1222(2009). Bunce, M.W., et al. J. Biol. Chem. 283(13):8678-8686(2008). Byun, B., et al. Biofactors 31 (3-4), 165-169 (2007).

NCBI and Uniprot Product Information

• NCBI GI #: 4507183
• NCBI GeneID: 8405
• NCBI Accession #: NP_003554.1
• NCBI GenBank Nucleotide #: NP_003554.1
• UniProt Accession #: O43791
• NCBI Official Full Name: speckle-type POZ protein
• NCBI Official Synonym Full Names: speckle type BTB/POZ protein
• NCBI Official Symbol: SPOP
• NCBI Official Synonym Symbols: TEF2; BTBD32; NEDMACE; NEDMIDF
• NCBI Protein Information: speckle-type POZ protein
• UniProt Protein Name: Speckle-type POZ protein
• Protein Family: Speckle-type POZ protein
• UniProt Gene Name: SPOP
• UniProt Entry Name: SPOP_HUMAN

NCBI Description

This gene encodes a protein that may modulate the transcriptional repression activities of death-associated protein 6 (DAXX), which interacts with histone deacetylase, core histones, and other histone-associated proteins. In mouse, the encoded protein binds to the putative leucine zipper domain of macroH2A1.2, a variant H2A histone that is enriched on inactivated X chromosomes. The BTB/POZ domain of this protein has been shown in other proteins to mediate transcriptional repression and to interact with components of histone deacetylase co-repressor complexes. Alternative splicing of this gene results in multiple transcript variants encoding the same protein. [provided by RefSeq, Jul 2008]

Uniprot Description

SPOP: Inhibits IPF1/PDX1 transactivation of established target promoters, such as insulin, may be by recruiting a repressor complex. In complex with CUL3, involved in ubiquitination of BMI1, H2AFY and DAXX, and probably also in ubiquitination and proteasomal degradation of Gli2 or Gli3. Belongs to the Tdpoz family.

Chromosomal Location of Human Ortholog: 17q21.33

Cellular Component: nucleoplasm; nuclear speck; nucleus

Molecular Function: protein binding; ubiquitin protein ligase binding

Biological Process: proteasomal ubiquitin-dependent protein catabolic process; protein ubiquitination during ubiquitin-dependent protein catabolic process; glucose homeostasis