IgG (H&L), F(ab)'2 fragment Secondary Antibody
Goat anti-Rat IgG (H&L), F(ab)'2 fragment, FITC Conjugate, min x w/bovine, goat, hamster, horse, human, mouse, or rabbit lgG/serum
Reactivity
Rat.Cross Reactivity: Based on IEP, no reactivity is observed to:
non-immunoglobulin rat serum proteins
IgG from goat, hamster, human, mouse, or rabbit
serum from bovine, goat, horse, human, mouse, or rabbit
Applications
Flow Cytometry, Functional Assay, Immunofluorescence, ELISA
Purity
> 90% based on SDS-PAGE Small amounts of intact IgG may be present.
Synonyms
IgG (H&L); F(ab)'2 fragment; Goat anti-Rat IgG (H&L); FITC Conjugate; min x w/bovine; goat; hamster; horse; human; mouse; or rabbit lgG/serum; Goat anti-rat IgG (H&L); F(ab)'2 fragment -Affinity Pure; F(ab)'2 fragment secondary antibody
Host
Goat
Reactivity
Rat.
Cross Reactivity: Based on IEP, no reactivity is observed to:
non-immunoglobulin rat serum proteins
IgG from goat, hamster, human, mouse, or rabbit
serum from bovine, goat, horse, human, mouse, or rabbit
Cross Reactivity: Based on IEP, no reactivity is observed to:
non-immunoglobulin rat serum proteins
IgG from goat, hamster, human, mouse, or rabbit
serum from bovine, goat, horse, human, mouse, or rabbit
Purity/Purification
> 90% based on SDS-PAGE Small amounts of intact IgG may be present.
Form/Format
Clear, fluorescent yellow liquid
Concentration
1.50 mg/ml (E 1% at 280 nm = 13.0) (varies by lot)
Applicable Applications for IgG (H&L), F(ab)'2 fragment secondary antibody
Flow Cytometry (FC/FACS), Immunofluorescence (IF), ELISA (EIA), Immunomicroscopy (IM)
Conjugate
Fluorescein-5-isothiocyanate (FITC) Amax = 494 nm; Emax = 518 nm
Immunogen
Purified Rat IgG, whole molecule
Buffer
30 mM Triethanolamine, pH 7.2, 5 mM Magnesium Chloride, 0.1 mM Zinc Chloride, 1 % (w/v) BSA, Protease/IgG free
Country of Origin
Goat serum was obtained from healthy animals of US origin and under the care of a registered veterinarian.
Preparation and Storage
Store undiluted liquid at 2-8 degree C. For storage at -20 degree C, dilute with an equal volume of glycerol to prevent loss of enzymatic activity. Prepare working dilutions prior to use and then discard.
Shelf Life: 1 year from date of receipt. Prepare working dilution prior to use and then discard.
Shelf Life: 1 year from date of receipt. Prepare working dilution prior to use and then discard.
Product Categories/Family for IgG (H&L), F(ab)'2 fragment secondary antibody
Similar Products
Product Notes
The IgG (H&L), F(ab)'2 fragment (Catalog #AAA686072) is a Secondary Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-Rat IgG (H&L), F(ab)'2 fragment, FITC Conjugate, min x w/bovine, goat, hamster, horse, human, mouse, or rabbit lgG/serum reacts with Rat. Cross Reactivity: Based on IEP, no reactivity is observed to: non-immunoglobulin rat serum proteins IgG from goat, hamster, human, mouse, or rabbit serum from bovine, goat, horse, human, mouse, or rabbit and may cross-react with other species as described in the data sheet. AAA Biotech's IgG (H&L), F(ab)'2 fragment can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), Immunofluorescence (IF), ELISA (EIA), Immunomicroscopy (IM). Researchers should empirically determine the suitability of the IgG (H&L), F(ab)'2 fragment for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "IgG (H&L), F(ab)'2 fragment, Secondary Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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