Goat Apolipoprotein AII Antibody | anti-Apo AII antibody

HRP-conjugated Goat Anti-Human Apolipoprotein AII

Cat. Num. AAA390014

• Gene Names: APOA2; apoAII; Apo-AII; ApoA-II
• Applications: ELISA, Immunoblot, Western Blot
• Synonyms: Apolipoprotein AII; HRP-conjugated Goat Anti-Human Apolipoprotein AII; Horseradish Peroxidase (HRP) conjugated Goat Anti-Human Apolipoprotein AII (Apo AII); Polyclonal Antibody; anti-Apo AII antibody

• Host: Goat
• Specificity: Specifically binds to human apo AII. Dilution for immunoblot and ELISA range: 200 to 6,000.
• Form/Format: 0.5 ml in Freeze-dried powder.
• Concentration: 1 mg / ml (varies by lot)
• Sequence Length: 100

• Applicable Applications for anti-Apo AII antibody: ELISA (EIA), Immunoblot (IB)
• Application Notes: Freeze-dried powder. Dilution for Immunoblot & ELISA range: 1,000 to 6,000.; Use: The antibody can be used for detection of apo AII in plasma and lipoproteins, immunoassays and immunoblots.
• Source Note: Polyclonal goat anti-human apo AII purified by human apo AII- Sepharose affinity column and conjugated with HRP.
• Preservative: 50 mM PBS, 0.1M NaCl, pH 7.4 and 0.01% Thimersol.
• Stabilizer: 10 mg / ml Bovine Serum Albumin.
• Preparation and Storage: Freeze-dried product should be stored refrigerated until opened. After opening, restore to 0.5 ml with distilled water. If it is not completely clear after standing for 1-2 Hours at room temperature, centrifuge the product. It is stable for several weeks at 4 degree C as an undiluted liquid. Do not use for more than one day after dilution. For extended storage after reconstitution, we suggest aliquot to avoid repeated freezing and thawing; or the addition of an equal volume of glycerol to make a final glycerol concentration of 50%, followed by storage at -20 degree C. The concentration of protein and buffer salts will decrease to one-half of the original after the addition of glycerol

Product Categories/Family for anti-Apo AII antibody

HRP Antibodies

NCBI and Uniprot Product Information

• NCBI GI #: 4502149
• NCBI GeneID: 336
• NCBI Accession #: NP_001634.1
• NCBI GenBank Nucleotide #: NM_001643.1
• UniProt Accession #: P02652; B2R524
• Molecular Weight: 11,175 Da
• NCBI Official Full Name: apolipoprotein A-II preproprotein
• NCBI Official Synonym Full Names: apolipoprotein A-II
• NCBI Official Symbol: APOA2
• NCBI Official Synonym Symbols: apoAII; Apo-AII; ApoA-II
• NCBI Protein Information: apolipoprotein A-II; apolipoprotein A2
• UniProt Protein Name: Apolipoprotein A-II
• UniProt Gene Name: APOA2
• UniProt Synonym Gene Names: Apo-AII; ApoA-II
• UniProt Entry Name: APOA2_HUMAN

NCBI Description

This gene encodes apolipoprotein (apo-) A-II, which is the second most abundant protein of the high density lipoprotein particles. The protein is found in plasma as a monomer, homodimer, or heterodimer with apolipoprotein D. Defects in this gene may result in apolipoprotein A-II deficiency or hypercholesterolemia. [provided by RefSeq, Jul 2008]

Uniprot Description

Function: May stabilize HDL (high density lipoprotein) structure by its association with lipids, and affect the HDL metabolism.

Subunit structure: Homodimer; disulfide-linked. Also forms a disulfide-linked heterodimer with APOD. Interacts with HCV core protein. Interacts with APOA1BP and NDRG1. Ref.15 Ref.16 Ref.19 Ref.23

Subcellular location: Secreted.

Tissue specificity: Plasma; synthesized in the liver and intestine.

Post-translational modification: Met-49 is oxidized to methionine sulfoxide.Phosphorylation sites are present in the extracellular medium.Apolipoprotein A-II is O-glycosylated. Ref.22

Polymorphism: A homozygous transition at position 1 of intron 3 of APOA2 results in deficiency of apolipoprotein A-II, without significant influence either on lipid and lipoprotein profiles or on the occurrence of coronary artery disease [

MIM:107670].

Sequence similarities: Belongs to the apolipoprotein A2 family.

Mass spectrometry: Molecular mass is 17252 Da from positions 24 - 100. Determined by ESI. Homodimer, without methionine sulfoxide. Ref.17Molecular mass is 17269 Da from positions 24 - 100. Determined by ESI. Homodimer, with 1 methionine sulfoxide, oxidation at Met-49. Ref.17Molecular mass is 8701.2 Da from positions 24 - 100. Determined by MALDI. Ref.18Molecular mass is 8823.4 Da from positions 24 - 100. Determined by MALDI. Cysteinylated ApoA-II. Ref.18Molecular mass is 17421.3 Da from positions 24 - 100. Determined by MALDI. Homodimer. Ref.18Molecular mass is 17293.4 Da from positions 24 - 100. Determined by MALDI. Heterodimer with truncated apolipoprotein A-II. Ref.18Molecular mass is 8578.3 Da from positions 24 - 99. Determined by MALDI. Ref.18Molecular mass is 17166.2 Da from positions 24 - 99. Determined by MALDI. Homodimer. Ref.18