Canine Anti-gonadotropin-releasing hormone receptor antibody ELISA Kit | GnRHR elisa kit

Canine Anti-gonadotropin-releasing hormone receptor antibody ELISA Kit

Cat. Num. AAA755411

• Gene Names: GNRHR; HH7; GRHR; LRHR; LHRHR; GNRHR1
• Reactivity: Canine
• Synonyms: Anti-gonadotropin-releasing hormone receptor antibody; Canine Anti-gonadotropin-releasing hormone receptor antibody ELISA Kit; GnRHR elisa kit

• Reactivity: Canine

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Detection Range: 100-1000ng/mL
• Sensitivity: 1.0ng/mL
• Intended Uses: This GNRHR-Ab ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Canine GNRHR-Ab. This ELISA kit for research use only, not for therapeutic applications
• !Intended Uses: This GNRHR-Ab ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Canine GNRHR-Ab. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for GnRHR elisa kit

Principle of the Assay: GNRHR-Ab ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-GNRHR-Ab antibody and an GNRHR-Ab-HRP conjugate. The assay sample and buffer are incubated together with GNRHR-Ab-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the GNRHR-Ab concentration since GNRHR-Ab from samples and GNRHR-Ab-HRP conjugate compete for the anti-GNRHR-Ab antibody binding site. Since the number of sites is limited, as more sites are occupied by GNRHR-Ab from the sample, fewer sites are left to bind GNRHR-Ab-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The GNRHR-Ab concentration in each sample is interpolated from this standard curve.

Product Categories/Family for GnRHR elisa kit

Neuroscience

NCBI and Uniprot Product Information

• NCBI GI #: 61676184
• NCBI GeneID: 2798
• NCBI Accession #: NP_001012781.1
• NCBI GenBank Nucleotide #: NM_001012763.1
• Molecular Weight: 27,727 Da
• NCBI Official Full Name: gonadotropin-releasing hormone receptor isoform 2
• NCBI Official Synonym Full Names: gonadotropin-releasing hormone receptor
• NCBI Official Symbol: GNRHR
• NCBI Official Synonym Symbols: HH7; GRHR; LRHR; LHRHR; GNRHR1
• NCBI Protein Information: gonadotropin-releasing hormone receptor; gnRH-R; gnRH receptor; luliberin receptor; type I GnRH receptor; leutinizing-releasing hormone receptor; leutinizing hormone releasing horomone receptor; gonadotropin-releasing hormone (type 1) receptor 1
• UniProt Protein Name: Gonadotropin-releasing hormone receptor
• Protein Family: Gonadotropin-releasing hormone receptor
• UniProt Gene Name: GNRHR
• UniProt Synonym Gene Names: GRHR; GnRH receptor; GnRH-R
• UniProt Entry Name: GNRHR_HUMAN

NCBI Description

This gene encodes the receptor for type 1 gonadotropin-releasing hormone. This receptor is a member of the seven-transmembrane, G-protein coupled receptor (GPCR) family. It is expressed on the surface of pituitary gonadotrope cells as well as lymphocytes, breast, ovary, and prostate. Following binding of gonadotropin-releasing hormone, the receptor associates with G-proteins that activate a phosphatidylinositol-calcium second messenger system. Activation of the receptor ultimately causes the release of gonadotropic luteinizing hormone (LH) and follicle stimulating hormone (FSH). Defects in this gene are a cause of hypogonadotropic hypogonadism (HH). Alternative splicing results in multiple transcript variants encoding different isoforms. More than 18 transcription initiation sites in the 5' region and multiple polyA signals in the 3' region have been identified for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

GNRHR: Receptor for gonadotropin releasing hormone (GnRH) that mediates the action of GnRH to stimulate the secretion of the gonadotropic hormones luteinizing hormone (LH) and follicle- stimulating hormone (FSH). This receptor mediates its action by association with G-proteins that activate a phosphatidylinositol- calcium second messenger system. Isoform 2 may act as an inhibitor of GnRH-R signaling. Defects in GNRHR are a cause of idiopathic hypogonadotropic hypogonadism (IHH). IHH is defined as a deficiency of the pituitary secretion of follicle-stimulating hormone and luteinizing hormone, which results in the impairment of pubertal maturation and of reproductive function. Defects in GNRHR are a cause of fertile eunuch syndrome (FEUNS). Fertile eunuch syndrome is a mild phenotypic form of HH going with the presence of normal testicular size and some degree of spermatogenesis. Belongs to the G-protein coupled receptor 1 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Receptor, GPCR; GPCR, family 1; Membrane protein, integral; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 4q21.2

Cellular Component: membrane; integral to plasma membrane; plasma membrane

Molecular Function: gonadotropin-releasing hormone receptor activity; peptide binding

Biological Process: G-protein coupled receptor protein signaling pathway; multicellular organismal development

Disease: Hypogonadotropic Hypogonadism 7 With Or Without Anosmia