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Glutathione S Transferase, pi, Western Blot Positive Control Recombinant Protein

Glutathione S Transferase, pi, Recombinant, Human, Western Blot Positive Control (GSTP1, GSTpi1, GST3, DFN7)

Applications
Western Blot
Purity
Purified
Purified (> 95%)
Synonyms
Glutathione S Transferase; pi; Western Blot Positive Control; Recombinant; Human; Western Blot Positive Control (GSTP1; GSTpi1; GST3; DFN7); Western Blot Positive Control recombinant protein
Ordering
For Research Use Only!
Purity/Purification
Purified
Purified (> 95%)
Form/Format
Supplied as a liquid in SDS-PAGE sample buffer (reduced).
Applicable Applications for Glutathione S Transferase, pi, Western Blot Positive Control recombinant protein
Western Blot (WB)
Application Notes
Suitable for use in Western Blot as a positive control.
Dilution: To visualize (load 10ul/lane) with antibodies . Heat once prior to loading on gels (10ul/lane).
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for 6 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Related Product Information for Glutathione S Transferase, pi, Western Blot Positive Control recombinant protein
GST's are responsible for the metabolism of a broad range of xenobiotics and carcinogens. The enzyme catalyzes the reaction of glutathione with a wide variety of organic compounds to form thioethers, a reaction that is sometimes a first step in a detoxification process leading to mercapturic acid formation. GSTs play an important role defense against electrophilic chemicals generated by cellular oxidative reactions catalyzed by cytochrome P450 and other oxidases. GSTs are believed to defend against the highly reactive electrophiles that are formed as a result of cellular oxidative processes. Based on amino acid sequence similarities and antibody cross-reactivities, the mammalian cytosolic GSTs are divided into the following classes: alpha, mu, kappa, theta, pi, zeta, omega and sigma. GSTs exists as homodimers of ~25kD. GST A1-1 is mainly found in kidney, intestine, lung, and liver. GST M1-1 is restricted to liver and few other tissues. GST P1-1 has a widespread distribution in most tumor cells and tissues but it is absent in liver. The GST M1-1 is the type b allelic variant and corresponds to human GST Y.
Product Categories/Family for Glutathione S Transferase, pi, Western Blot Positive Control recombinant protein

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Product Notes

The Glutathione S Transferase, pi, Western Blot Positive Control (Catalog #AAA636340) is a Recombinant Protein and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Glutathione S Transferase, pi, Western Blot Positive Control can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Suitable for use in Western Blot as a positive control. Dilution: To visualize (load 10ul/lane) with antibodies. Heat once prior to loading on gels (10ul/lane). Researchers should empirically determine the suitability of the Glutathione S Transferase, pi, Western Blot Positive Control for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Glutathione S Transferase, pi, Western Blot Positive Control, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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