Typical Testing Data/Standard Curve (for reference only)
Chicken Glucose Transporter 2 ELISA Kit | GLUT2 elisa kit
Chicken Glucose Transporter 2 ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: GLUT2 ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-GLUT2 antibody and an GLUT2-HRP conjugate. The assay sample and buffer are incubated together with GLUT2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the GLUT2 concentration since GLUT2 from samples and GLUT2-HRP conjugate compete for the anti-GLUT2 antibody binding site. Since the number of sites is limited, as more sites are occupied by GLUT2 from the sample, fewer sites are left to bind GLUT2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The GLUT2 concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
Uniprot Description
GLUT2: an integral membrane facilitative glucose transporter. One of 13 members of the human equilibrative glucose transport protein family. Present at high levels in the plasma membrane of pancreatic b-cells where it initiates the first step in glucose-stimulated insulin secretion, a glucose metabolism-dependent signaling mechanism. May mediate the bidirectional transfer of glucose across the plasma membrane of hepatocytes. May also participate with the Na( +2)/glucose cotransporter in the transcellular transport of glucose in the small intestine and kidney. Required for glucose sensitivity in the hypothalamus and brain stem. Involved in the control of food intake and stimulation of glucose uptake by peripheral tissues.
Protein type: Membrane protein, multi-pass; Membrane protein, integral; Transporter; Transporter, SLC family
Cellular Component: membrane; brush border membrane; basolateral plasma membrane; cytoplasm; integral to membrane; plasma membrane; cytosol; endosome; brush border
Molecular Function: D-glucose transmembrane transporter activity; dehydroascorbic acid transporter activity; transporter activity; glucose transmembrane transporter activity; transmembrane transporter activity; substrate-specific transmembrane transporter activity; insulin receptor binding
Biological Process: carbohydrate utilization; transport; carbohydrate transport; glucose transport; transmembrane transport
Research Articles on GLUT2
Similar Products
Product Notes
The Chicken GLUT2 slc2a2 (Catalog #AAA751265) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA751265 ELISA Kit recognizes Chicken GLUT2. It is sometimes possible for the material contained within the vial of "Glucose Transporter 2, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
