GLO1 recombinant protein

GLO1 Protein, Mouse, Recombinant (His Tag)

Cat. Num. AAA8122706

• Gene Names: Glo1; GLY1; Qglo; Glo-1; Glo-1r; Glo-1s; Glo1-r; Glo1-s; AW550643; 0610009E22Rik; 1110008E19Rik; 2510049H23Rik
• Purity: >85% as determined by SDS-PAGE
• Synonyms: GLO1; GLO1 Protein; Mouse; Recombinant (His Tag); Mouse GLO1/Glyoxalase 1 Protein (His Tag); 0610009E22Rik Protein; 1110008E19Rik Protein; 2510049H23Rik Protein; AW550643 Protein; Glo-1 Protein; Glo-1r Protein; Glo-1s Protein; Glo1-r Protein; Glo1-s Protein; GLY1 Protein; Qglo Protein; glyoxalase I; GLO1 recombinant protein

• Host: E Coli
• Purity/Purification: >85% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Ala2-Ile184

• Species: Mouse
• Predicted N Terminal: Met
• Tag: N-His
• Protein Construction: A DNA sequence encoding the mouse GLO1 (NP_079650.3)(Ala 2-Ile 184) was expressed, with a polyhistide tag at the N-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for GLO1 recombinant protein

Background: Lactoylglutathione lyase, also known as Methylglyoxalase, Aldoketomutase, Glyoxalase I, Ketone-aldehyde mutase, S-D-lactoylglutathione methylglyoxal lyase and GLO1, is a member of the glyoxalase I family. GLO1/Glyoxalase I is a ubiquitous cellular defense enzyme involved in the detoxification of methylglyoxal, a cytotoxic byproduct of glycolysis. Accumulative evidence suggests an important role of GLO1 expression in protection against methylglyoxal-dependent protein adduction and cellular damage associated with diabetes, cancer, and chronological aging. GLO1/Glyoxalase I has been implicated in anxiety-like behavior in mice and in multiple psychiatric diseases in humans. GLO1/Glyoxalase I catalyzes the conversion of hemimercaptal, formed from methylglyoxal and glutathione, to S-lactoylglutathione. GLO1/Glyoxalase I exists in three separable isoforms which originate from two alleles in the genome. These correspond to two homodimers and one heterodimer composed of two subunits showing different electrophoretic properties. GLO1 upregulation may play a functional role in glycolytic adaptations of cancer cells.

References

Wu, YY. et al., 2008,Prog Neuropsychopharmacol Biol Psychiatry. 32 (7):1740-4. Williams,R. et al., 2009, PLoS One 4 (3): e4649. Antognelli,C. et al., 2009, BMC Cancer 9 :115. Bair,W.B. et al., 2010, Melanoma Res 20 (2):85-96.

NCBI and Uniprot Product Information

• NCBI GI #: 165932331
• NCBI GeneID: 109801
• NCBI Accession #: NP_079650.3
• NCBI GenBank Nucleotide #: NM_025374.3
• UniProt Accession #: Q9CPU0; Q543L3; Q8R3T1
• Molecular Weight: 20,810 Da
• NCBI Official Full Name: lactoylglutathione lyase
• NCBI Official Synonym Full Names: glyoxalase 1
• NCBI Official Symbol: Glo1
• NCBI Official Synonym Symbols: GLY1; Qglo; Glo-1; Glo-1r; Glo-1s; Glo1-r; Glo1-s; AW550643; 0610009E22Rik; 1110008E19Rik; 2510049H23Rik
• NCBI Protein Information: lactoylglutathione lyase
• UniProt Protein Name: Lactoylglutathione lyase
• UniProt Gene Name: Glo1
• UniProt Synonym Gene Names: Glx I

Uniprot Description

GLO1: Catalyzes the conversion of hemimercaptal, formed from methylglyoxal and glutathione, to S-lactoylglutathione. Involved in the regulation of TNF-induced transcriptional activity of NF- kappa-B. Belongs to the glyoxalase I family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Carbohydrate Metabolism - pyruvate; EC 4.4.1.5; Lyase

Chromosomal Location of Human Ortholog: 17 A3.3|17 15.69 cM

Molecular Function: lactoylglutathione lyase activity; zinc ion binding

Biological Process: carbohydrate metabolic process; glutathione metabolic process; methylglyoxal catabolic process to D-lactate; methylglyoxal metabolic process; negative regulation of apoptosis; osteoclast differentiation; regulation of transcription from RNA polymerase II promoter