GLI Family Zinc Finger Protein 1 Recombinant Protein | GLI1 recombinant protein

Recombinant GLI Family Zinc Finger Protein 1 (GLI1)

Cat. Num. AAA2030327

• Gene Names: GLI1; GLI
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: >95%
• Synonyms: GLI Family Zinc Finger Protein 1; Recombinant GLI Family Zinc Finger Protein 1 (GLI1); GLI1 recombinant protein

• Host: Host: E Coli; Source: Prokaryotic expression
• Purity/Purification: >95%
• Form/Format: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.; V QVKPEQGCPV GSDSTGLAPC LNAHPSEGSP DPQPLFSHHS QLPPPQYLQS GPYPQPPHDC LSLESRPGLN FNPSSHSTGQ LKAQLLCNYV QSQQELLWEG RSRGGIPNQE LPYQSPKFLG GSQVSQSPTK VPAAAAAATF GSGFASPALA NHKSVSYPAP SPCHETFTMG VNRPPHRPAA PPRLLPPLPP CYGPLKVGDA NPSCGHPEVG RLGAGPALYP PPEGQVCNPL DSLDLDNTQL DFVAILDEAQ GLSPPPSHEQ GDSSKNTPPP SGPPNMAVGNMSVLLGSLPG ETQFLNSSA
• Sequence Length: 1106

• Applicable Applications for GLI1 recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Predicted Molecular Mass: 35.0kDa
• Accurate Molecular Mass (KD): 44kDa
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Expression System: Prokaryotic expression
• Tag: N-terminal His-Tag, T7 tag
• Organism Species: Rattus norvegicus (Rat)
• Fragment: Val5~Glu130
• Usage: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. The loss of this protein is less than 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 20152843
• NCBI GeneID: 2735
• Molecular Weight: 104,627 Da
• NCBI Official Full Name: GLI1
• NCBI Official Synonym Full Names: GLI family zinc finger 1
• NCBI Official Symbol: GLI1
• NCBI Official Synonym Symbols: GLI
• NCBI Protein Information: zinc finger protein GLI1
• UniProt Protein Name: Zinc finger protein GLI1
• Protein Family: Zinc finger protein
• UniProt Gene Name: GLI1
• UniProt Synonym Gene Names: GLI
• UniProt Entry Name: GLI1_HUMAN

NCBI Description

This gene encodes a member of the Kruppel family of zinc finger proteins. The encoded transcription factor is activated by the sonic hedgehog signal transduction cascade and regulates stem cell proliferation. The activity and nuclear localization of this protein is negatively regulated by p53 in an inhibitory loop. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2009]

Uniprot Description

GLI1: Acts as a transcriptional activator. May regulate the transcription of specific genes during normal development. May play a role in craniofacial development and digital development, as well as development of the central nervous system and gastrointestinal tract. Mediates SHH signaling and thus cell proliferation and differentiation. Interacts with KIF7. Interacts with ZIC1; the interaction enhances transcription activation. Amplified in glioblastoma cells. Testis, myometrium and fallopian tube. Also expressed in the brain with highest expression in the cerebellum, optic nerve and olfactory tract. Belongs to the GLI C2H2-type zinc-finger protein family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Transcription factor; C2H2-type zinc finger protein; Oncoprotein

Chromosomal Location of Human Ortholog: 12q13.2-q13.3

Cellular Component: nucleoplasm; intracellular membrane-bound organelle; cytoplasm; nucleus; cytosol

Molecular Function: RNA polymerase II transcription factor activity, enhancer binding; protein binding; DNA binding; microtubule binding; metal ion binding; chromatin binding

Biological Process: transcription from RNA polymerase II promoter; smoothened signaling pathway; ventral midline development; smoothened signaling pathway in regulation of granule cell precursor cell proliferation; positive regulation of transcription, DNA-dependent; digestive tract morphogenesis; notochord regression; osteoblast differentiation; dorsal/ventral pattern formation; cerebellar cortex morphogenesis; pituitary gland development; positive regulation of cell proliferation; positive regulation of smoothened signaling pathway; epidermal cell differentiation; positive regulation of transcription from RNA polymerase II promoter; spermatogenesis; proximal/distal pattern formation; positive regulation of DNA replication; regulation of smoothened signaling pathway; lung development