Human Ghrelin ELISA Kit | GHRL elisa kit
Human Ghrelin (GHRL) ELISA Kit
Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate GHRL in Human serum, plasma. An antibody specific for GHRL has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any GHRL present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for GHRL is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of GHRL bound in the initial step. The color development is stopped and the intensity of the color is measured.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes the ghrelin-obestatin preproprotein that is cleaved to yield two peptides, ghrelin and obestatin. Ghrelin is a powerful appetite stimulant and plays an important role in energy homeostasis. Its secretion is initiated when the stomach is empty, whereupon it binds to the growth hormone secretagogue receptor in the hypothalamus which results in the secretion of growth hormone (somatotropin). Ghrelin is thought to regulate multiple activities, including hunger, reward perception via the mesolimbic pathway, gastric acid secretion, gastrointestinal motility, and pancreatic glucose-stimulated insulin secretion. It was initially proposed that obestatin plays an opposing role to ghrelin by promoting satiety and thus decreasing food intake, but this action is still debated. Recent reports suggest multiple metabolic roles for obestatin, including regulating adipocyte function and glucose metabolism. Alternative splicing results in multiple transcript variants. In addition, antisense transcripts for this gene have been identified and may potentially regulate ghrelin-obestatin preproprotein expression. [provided by RefSeq, Nov 2014]
Uniprot Description
ghrelin: a hormone that binds to the growth hormone secretagogue receptor type 1 (GHSR). Secreted by the stomach
Protein type: Apoptosis; Cell cycle regulation; Cell development/differentiation; Secreted; Secreted, signal peptide
Chromosomal Location of Human Ortholog: 3p25.3
Cellular Component: axon; endoplasmic reticulum lumen; extracellular region; extracellular space
Molecular Function: G-protein coupled receptor binding; ghrelin receptor binding; growth hormone-releasing hormone activity; protein binding; protein tyrosine kinase activator activity
Biological Process: actin polymerization or depolymerization; activation of MAPK activity; adult feeding behavior; cartilage development; cortisol secretion; decidualization; dendrite development; elevation of cytosolic calcium ion concentration; excitatory postsynaptic potential; G-protein coupled receptor signaling pathway; gastric acid secretion; glucose metabolic process; growth hormone secretion; hormone-mediated signaling pathway; negative regulation of angiogenesis; negative regulation of apoptosis; negative regulation of circadian sleep/wake cycle, REM sleep; negative regulation of endothelial cell proliferation; negative regulation of inflammatory response; negative regulation of insulin secretion; negative regulation of interleukin-1 beta production; negative regulation of interleukin-6 biosynthetic process; negative regulation of locomotion; negative regulation of tumor necrosis factor biosynthetic process; positive regulation of appetite; positive regulation of circadian sleep/wake cycle, non-REM sleep; positive regulation of corticotropin secretion; positive regulation of cortisol secretion; positive regulation of growth hormone secretion; positive regulation of growth rate; positive regulation of insulin secretion; positive regulation of multicellular organism growth; positive regulation of synaptogenesis; regulation of cell proliferation; regulation of response to food; regulation of transmission of nerve impulse; response to electrical stimulus; response to estrogen; response to hormone
Disease: Obesity
Research Articles on GHRL
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Product Notes
The Human GHRL ghrl (Catalog #AAA283919) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA283919 ELISA Kit recognizes Human GHRL. It is sometimes possible for the material contained within the vial of "Ghrelin, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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