Guinea Pig Growth hormone releasing hormone ELISA Kit | GHRH elisa kit

Guinea pig Growth hormone releasing hormone ELISA Kit

Cat. Num. AAA720195

• Gene Names: GHRHR; GRFR; GHRFR; IGHD1B
• Reactivity: Guinea Pig
• Synonyms: Growth hormone releasing hormone; Guinea pig Growth hormone releasing hormone ELISA Kit; GHRH elisa kit

• Reactivity: Guinea Pig

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Detection Range: 50-1000pg/mL
• Sensitivity: 1.0pg/mL
• Intended Uses: This GHRH ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Guinea pig GHRH. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for GHRH elisa kit

Principle of the assay: GHRH ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-GHRH antibody and an GHRH-HRP conjugate. The assay sample and buffer are incubated together with GHRH-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the GHRH concentration since GHRH from samples and GHRH-HRP conjugate compete for the anti-GHRH antibody binding site. Since the number of sites is limited, as more sites are occupied by GHRH from the sample, fewer sites are left to bind GHRH-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The GHRH concentration in each sample is interpolated from this standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 1706945
• NCBI GeneID: 2692
• Molecular Weight: 47,402 Da
• NCBI Official Full Name: growth hormone releasing hormone, partial
• NCBI Official Synonym Full Names: growth hormone releasing hormone receptor
• NCBI Official Symbol: GHRHR
• NCBI Official Synonym Symbols: GRFR; GHRFR; IGHD1B
• NCBI Protein Information: growth hormone-releasing hormone receptor; GRF receptor; GHRH receptor
• UniProt Protein Name: Growth hormone-releasing hormone receptor
• Protein Family: Somatoliberin
• UniProt Gene Name: GHRHR
• UniProt Synonym Gene Names: GHRH receptor; GRF receptor; GRFR
• UniProt Entry Name: GHRHR_HUMAN

NCBI Description

This gene encodes a receptor for growth hormone-releasing hormone. Binding of this hormone to the receptor leads to synthesis and release of growth hormone. Mutations in this gene have been associated with isolated growth hormone deficiency (IGHD), also known as Dwarfism of Sindh, a disorder characterized by short stature. [provided by RefSeq, Jun 2010]

Uniprot Description

GHRHR: Receptor for GRF, coupled to G proteins which activate adenylyl cyclase. Stimulates somatotroph cell growth, growth hormone gene transcription and growth hormone secretion. Defects in GHRHR are a cause of growth hormone deficiency isolated type 1B (IGHD1B); also known as dwarfism of Sindh. IGHD1B is an autosomal recessive deficiency of GH which causes short stature. IGHD1B patients have low but detectable levels of GH. Belongs to the G-protein coupled receptor 2 family.

Protein type: Membrane protein, integral; GPCR, family 2; Receptor, GPCR; Cell development/differentiation; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 7p14

Cellular Component: nuclear outer membrane; nuclear matrix; cell surface; cytoplasm; plasma membrane; integral to membrane; nuclear inner membrane; secretory granule

Molecular Function: G-protein coupled receptor activity; protein binding; growth factor binding; peptide hormone binding; growth hormone-releasing hormone receptor activity

Biological Process: positive regulation of insulin-like growth factor receptor signaling pathway; lactation; adenylate cyclase activation; regulation of protein metabolic process; response to glucocorticoid stimulus; cell maturation; determination of adult life span; positive regulation of multicellular organism growth; reproductive process in a multicellular organism; G-protein signaling, adenylate cyclase activating pathway; response to insulin stimulus; water homeostasis; positive regulation of growth hormone secretion; growth hormone secretion; cAMP-mediated signaling; cellular response to insulin stimulus; response to estrogen stimulus; positive regulation of cAMP biosynthetic process; somatotropin secreting cell development; positive regulation of cell proliferation; hormone metabolic process; regulation of steroid hormone receptor signaling pathway

Disease: Isolated Growth Hormone Deficiency, Type Ib