Canine Growth hormone releasing factor ELISA Kit | GH-RF elisa kit

Canine growth hormone releasing factor (GH-RF) ELISA Kit

Cat. Num. AAA2607220

• Gene Names: GHRH; GRF; INN; GHRF
• Reactivity: Canine
• Synonyms: Growth hormone releasing factor; Canine growth hormone releasing factor (GH-RF) ELISA Kit; GH-RF elisa kit

• Reactivity: Canine
• Specificity: No cross-reaction with other factors.
• Sequence Length: 107

• Samples: Serum, plasma or cell culture supernatant
• Assay Type: Quantitative Sandwich
• Detection Range: 10 ng/ml-0.156ng/ml
• Sensitivity: Up to 0.05 ng/ml.
• Intra-assay Precision: <= 8%
• Inter-assay Precision: <= 12%
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Standard Curve/Testing Data

Related Product Information for GH-RF elisa kit

Principle of the Assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Canine GH-RF monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

NCBI and Uniprot Product Information

• NCBI GI #: 337133
• NCBI GeneID: 2691
• Molecular Weight: 12,360 Da
• NCBI Official Full Name: growth hormone releasing factor, partial
• NCBI Official Synonym Full Names: growth hormone releasing hormone
• NCBI Official Symbol: GHRH
• NCBI Official Synonym Symbols: GRF; INN; GHRF
• NCBI Protein Information: somatoliberin
• UniProt Protein Name: Somatoliberin
• UniProt Gene Name: GHRH
• UniProt Synonym Gene Names: GHRF; GRF; GHRH
• UniProt Entry Name: SLIB_HUMAN

NCBI Description

This gene encodes a member of the glucagon family of proteins. The encoded preproprotein is produced in the hypothalamus and cleaved to generate the mature factor, known as somatoliberin, which acts to stimulate growth hormone release from the pituitary gland. Variant receptors for somatoliberin have been found in several types of tumors, and antagonists of these receptors can inhibit the growth of the tumors. Defects in this gene are a cause of dwarfism, while hypersecretion of the encoded protein is a cause of gigantism. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed. [provided by RefSeq, Jan 2016]

Uniprot Description

GHRH: GRF is released by the hypothalamus and acts on the adenohypophyse to stimulate the secretion of growth hormone. Belongs to the glucagon family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 20q11.2

Cellular Component: extracellular region; extracellular space; intracellular; terminal button

Molecular Function: growth hormone-releasing hormone activity; growth hormone-releasing hormone receptor binding

Biological Process: adenohypophysis development; cAMP-mediated signaling; cell-cell signaling; G-protein signaling, adenylate cyclase activating pathway; growth hormone secretion; positive regulation of cAMP biosynthetic process; positive regulation of cell proliferation; positive regulation of circadian sleep/wake cycle, REM sleep; positive regulation of growth hormone secretion; positive regulation of insulin-like growth factor receptor signaling pathway; positive regulation of multicellular organism growth; response to food