General Tartrazine (TZ) ELISA Kit | TZ elisa kit

General Tartrazine (TZ) ELISA Kit

Cat. Num. AAA2104988

• Gene Names: THI1; MBG8.3; MBG8_3; THI1 PROTEIN; THI4; THIAMINE4; THIAZOLE REQUIRING; TZ
• Reactivity: General
• Synonyms: Tartrazine (TZ); General Tartrazine (TZ) ELISA Kit; Trisodium 1-(4-sulfonatophenyl)-4-(4-sulfonatophenylazo)-5-pyrazolone-3-carboxylate); TZ elisa kit

• Reactivity: General
• Specificity: This assay has high sensitivity and excellent specificity for detection of T4. No significant cross-reactivity or interference between T4 and analogues was observed.
• Sequence Length: 349

• Application Notes: Enzyme-linked immunosorbent assay for Antigen Detection.
• Samples: Serum, Plasma And Other Biological Fluids
• Assay Type: Quantitative Competitive
• Detection Range: 3.70-300ng/mL
• Sensitivity: 1.29ng/mL.
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level T4 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level T4 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for TZ elisa kit

Intended Uses: The kit is a competitive inhibition enzyme immunoassay technique for the in vitro quantitative measurement of T4 in serum, plasma and other biological fluids.

Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to T4 has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled T4 and unlabeled T4 (Standards or samples) with the pre-coated antibody specific to T4. After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of T4 in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of T4 in the sample.

NCBI and Uniprot Product Information

• NCBI GI #: 15239735
• NCBI GeneID: 835567
• NCBI Accession #: NP_200288.1
• NCBI GenBank Nucleotide #: NM_124858.4
• UniProt Accession #: Q38814
• Molecular Weight: 29,362 Da
• NCBI Official Full Name: thiazole biosynthetic enzyme, chloroplast (ARA6) (THI1) (THI4)
• NCBI Official Symbol: THI1
• NCBI Official Synonym Symbols: MBG8.3; MBG8_3; THI1 PROTEIN; THI4; THIAMINE4; THIAZOLE REQUIRING; TZ
• NCBI Protein Information: thiazole biosynthetic enzyme, chloroplast (ARA6) (THI1) (THI4)
• UniProt Protein Name: Thiamine thiazole synthase, chloroplastic
• UniProt Gene Name: THI1
• UniProt Synonym Gene Names: ARA6; THI4

NCBI Description

Encodes a thiamine biosynthetic gene that has a dual function in thiamine biosynthesis and mitochondrial DNA damage tolerance. It appears to be involved in producing the thiazole portion of thiamine (vitamin B1). A crystal structure of the protein reveals that it forms a 2-ring homo-octamer.

Uniprot Description

Involved in biosynthesis of the thiamine precursor thiazole. Catalyzes the conversion of NAD and glycine to adenosine diphosphate 5-(2-hydroxyethyl)-4-methylthiazole-2-carboxylic acid (ADT), an adenylated thiazole intermediate. The reaction includes an iron-dependent sulfide transfer from a conserved cysteine residue of the protein to a thiazole intermediate. The enzyme can only undergo a single turnover, which suggests it is a suicide enzyme. May have additional roles in adaptation to various stress conditions and in DNA damage tolerance. Acts as a positive regulator for the abscisic acid-induced activation of slow type anion channels during stomatal closure by repressing CPK33 kinase activity.