General LTC4 (Leukotriene C4) ELISA Kit | LTC4 elisa kit

LTC4 (Leukotriene C4) ELISA Kit

Cat. Num. AAA2540381

• Reactivity: General
• Synonyms: LTC4 (Leukotriene C4); LTC4 (Leukotriene C4) ELISA Kit; LTC4 elisa kit

• Reactivity: General
• Specificity: This kit recognizes LTB4 in samples. No significant cross-reactivity or interference between LTB4and analogues was observed.
• Sequence Length: 150

• Samples: Serum, Plasma And Other Biological Fluids
• Assay Type: Quantitative Competitive
• Detection Range: 15.63-1000pg/mL
• Sensitivity: 9.38pg/mL.
• Preparation and Storage: Store at 4 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for LTC4 elisa kit

Intended Uses: This ELISA kit applies to the in vitro quantitative determination of LTB4 concentrations in serum, plasma and other biological fluids.

Principle of the Assay: This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with LTB4. During the reaction, LTB4 in the sample or standard competes with a fixed amount of LTB4 on the solid phase supporter for sites on the Biotinylated Detection Ab specific to LTB4. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) are added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of LTB4 in the samples is then determined by comparing the OD of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 22035629
• NCBI GeneID: 4056
• NCBI Accession #: NP_665874.1
• NCBI GenBank Nucleotide #: NM_145867.1
• Molecular Weight: 16,567 Da
• NCBI Official Full Name: leukotriene C4 synthase
• NCBI Official Synonym Full Names: leukotriene C4 synthase
• NCBI Official Symbol: LTC4S
• NCBI Protein Information: leukotriene C4 synthase
• UniProt Protein Name: Leukotriene C4 synthase
• UniProt Gene Name: LTC4S
• UniProt Synonym Gene Names: LTC4 synthase
• UniProt Entry Name: LTC4S_HUMAN

NCBI Description

The MAPEG (Membrane Associated Proteins in Eicosanoid and Glutathione metabolism) family includes a number of human proteins, several of which are involved the production of leukotrienes. This gene encodes an enzyme that catalyzes the first step in the biosynthesis of cysteinyl leukotrienes, potent biological compounds derived from arachidonic acid. Leukotrienes have been implicated as mediators of anaphylaxis and inflammatory conditions such as human bronchial asthma. This protein localizes to the nuclear envelope and adjacent endoplasmic reticulum. [provided by RefSeq, Jul 2008]

Uniprot Description

LTC4S: Catalyzes the conjugation of leukotriene A4 with reduced glutathione to form leukotriene C4. Defects in LTC4S are the cause of leukotriene C4 synthase deficiency (LTC4 synthase deficiency). LTC4 synthase deficiency is a fatal neurometabolic developmental disorder. It is associated with muscular hypotonia, psychomotor retardation, failure to thrive, and microcephaly. Belongs to the MAPEG family.

Protein type: Membrane protein, integral; Lyase; Endoplasmic reticulum; Nuclear envelope; Lipid Metabolism - arachidonic acid; Membrane protein, multi-pass; EC 4.4.1.20

Chromosomal Location of Human Ortholog: 5q35

Cellular Component: endoplasmic reticulum; intracellular membrane-bound organelle; nuclear envelope

Molecular Function: glutathione peroxidase activity; glutathione transferase activity; leukotriene-C4 synthase activity; lipid binding; protein binding

Biological Process: leukotriene biosynthetic process; leukotriene metabolic process; lipoxygenase pathway

Disease: Leukotriene C4 Synthase Deficiency