Rabbit anti-Human APE1/APEX1 Polyclonal Antibody | anti-APEX1 antibody

Anti-APE1/APEX1 Antibody, Rabbit Polyclonal

Cat. Num. AAA8106388

• Gene Names: APEX1; APE; APX; APE1; APEN; APEX; HAP1; REF1
• Reactivity: Human
• Applications: Western Blot, ELISA, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation
• Purity: Protein A & Antigen Affinity
• Synonyms: APE1/APEX1; Polyclonal Antibody; Anti-APE1/APEX1 Antibody; Rabbit Polyclonal; APEX1/APE1/Ref-1 Antibody; Rabbit PAb; Antigen Affinity Purified; APEX nuclease (multifunctional DNA repair enzyme) 1; Anti-APE Antibody; Anti-APE1 Antibody; Anti-APEN Antibody; Anti-APEX Antibody; Anti-APX Antibody; Anti-HAP1 Antibody; Anti-REF1 Antibody; anti-APEX1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human APEX1/APE1/Ref-1
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-APEX1 antibody: Western Blot (WB), ELISA (EIA), Immunohistochemistry-Paraffin (IHC-P), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP)
• Application Notes: WB: 1:500-1:2000; ELISA: 1:5000-1:10,000; IHC-P: 1:500-1:2000; ICC/IF: 1:300-1:10,000; IP: 1-4uL/mg of lysate
• Immunogen: Recombinant Human APEX1/APE1/Ref-1 Protein
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with purified, recombinant Human APEX1/APE1/Ref-1 (rh APEX1/APE1/Ref-1). APEX1/APE1/Ref-1 specific IgG was purified by Human APEX1/APE1/Ref-1 affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Anti-APEX1 rabbit polyclonal antibody at 1:500 dilutionLane A: A431 Whole Cell LysateLane B: HepG2 Whole Cell LysateLane C: HeLa Whole Cell LysateLane D: Raji Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti- Rabbit  IgG H&L (Dylight 800)  at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:36 kDaObserved band size:39 kDa)

Immunohistochemistry (IHC)

(Immunochemical staining of human APEX1 in human prostate with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).)

Immunohistochemistry (IHC)

(Immunochemical staining of human APEX1 in human breast with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).)

Immunofluorescence (IF)

(Immunofluorescence staining of APEX1 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-human APEX1 polyclonal antibody (1:1000) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green).Positive staining was localized to nucleus.)

Immunoprecipitation (IP)

(APEX1 was immunoprecipitated using:Lane A:0.5 mg Hela Whole Cell LysateLane B:0.5 mg HepG2 Whole Cell Lysate4 uL anti-APEX1 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-APEX1 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique.Performed under reducing conditions.Predicted band size: 36 kDaObserved band size: 36 kDa)

Related Product Information for anti-APEX1 antibody

The enzyme is known to be a redox factor (Ref-1) stimulating DNA binding activity of AP-1 binding proteins such as Fos and Jun as well as a multifunctional DNA repair enzyme having 5' AP endonuclease, DNA 3' repair diesterase, 3'-5' exonuclease and DNA 3'-phosphatase activities.Although Apex mRNA was expressed ubiquitously, the levels varied significantly, suggesting organ-or tissue-specific expression of the Apex gene. The highest level was observed in the testis, relatively high levels in the thymus, spleen, kidney and brain, and the lowest level in the liver in rats. However, the present results suggested that APEX/Ref-1 gene product can interact with AP-1 binding proteins in brain, especially in the hippocampal formation, to regulate some brain functions by redox-activation.

References

Ono Y, et al. (1995) Developmental expression of APEX nuclease, a multifunctional DNA repair enzyme, in mouse brains. Brain Res Dev Brain Res.86 (1-2): 1-6.Tan Y, et al. (1996) cDNA cloning of rat major AP endonuclease (APEX nuclease) and analyses of its mRNA expression in rat tissues. Acta Med Okayama. 50 (1): 53-60.Yao M, et al. (1999) Genomic structure of the rat major AP endonuclease gene (Apex) with an adjacent putative O-sialoglycoprotease gene (Prsmg1/Gcpl1) and a processed Apex pseudogene (Apexp1). Acta Med Okayama. 53 (6): 245-52.

NCBI and Uniprot Product Information

• NCBI GI #: 346644849
• NCBI GeneID: 328
• NCBI Accession #: NP_001231178.1
• NCBI GenBank Nucleotide #: NM_001244249.1
• UniProt Accession #: P27695; Q969L5; Q99775
• Molecular Weight: 35,555 Da
• NCBI Official Full Name: DNA-(apurinic or apyrimidinic site) lyase
• NCBI Official Synonym Full Names: APEX nuclease (multifunctional DNA repair enzyme) 1
• NCBI Official Symbol: APEX1
• NCBI Official Synonym Symbols: APE; APX; APE1; APEN; APEX; HAP1; REF1
• NCBI Protein Information: DNA-(apurinic or apyrimidinic site) lyase; DNA-(apurinic or apyrimidinic site) lyase; AP lyase; protein REF-1; redox factor-1; AP endonuclease class I; apurinic-apyrimidinic endonuclease 1; apurinic/apyrimidinic (abasic) endonuclease; deoxyribonuclease (a
• UniProt Protein Name: DNA-(apurinic or apyrimidinic site) lyase
• Protein Family: DNA-(apurinic or apyrimidinic site) lyase
• UniProt Gene Name: APEX1
• UniProt Synonym Gene Names: APE; APE1; APEX; APX; HAP1; REF1; APEN; AP endonuclease 1; APE-1
• UniProt Entry Name: APEX1_HUMAN

NCBI Description

Apurinic/apyrimidinic (AP) sites occur frequently in DNA molecules by spontaneous hydrolysis, by DNA damaging agents or by DNA glycosylases that remove specific abnormal bases. AP sites are pre-mutagenic lesions that can prevent normal DNA replication so the cell contains systems to identify and repair such sites. Class II AP endonucleases cleave the phosphodiester backbone 5' to the AP site. This gene encodes the major AP endonuclease in human cells. Splice variants have been found for this gene; all encode the same protein. [provided by RefSeq, Jul 2008]

Uniprot Description

APE1: a multifunctional enzyme that plays a central role in the cellular response to oxidative stress including DNA repair and redox regulation of transcriptional factors. Binds DNA and RNA. Functions as an apurinic/apyrimidinic (AP) endodeoxyribonuclease in the DNA base excision repair (BER) pathway, a 3'-5' exoribonuclease for mismatched deoxyribonucleotides at the 3' termini of nicked or gapped DNA molecules, and a DNA 3' phosphodiesterase capable of removing lesions (such as phosphoglycolate) blocking the 3' side of DNA strand breaks. Is a loading factor for POLB onto non-incised AP sites in DNA, stimulates the 5'-terminal deoxyribose 5'- phosphate (dRp) excision activity of POLB, and involved in the DNA cleavage step of class switch recombination (CSR). Possesses reversible nuclear redox activity to regulate DNA binding affinity and transcriptional activity of transcriptional factors by controlling the redox status of their DNA-binding domain, such as the FOS/JUN AP-1 complex after exposure to IR. Binds to negative calcium response elements (nCaREs). Stimulates the YBX1-mediated MDR1 promoter activity, when acetylated at Lys-6 and Lys-7, leading to drug resistance. Is an endoribonuclease involved in the control of single-stranded RNA metabolism. Plays a role in regulating MYC mRNA turnover. In association with NMD1, plays a role in the rRNA quality control process during cell cycle progression. Interacts with SIRT1; the interaction is increased in the context of genotoxic stress. Interacts with HDAC1, HDAC2 and HDAC3; the interactions are not dependent on the APEX1 acetylation status. Up-regulated in presence of reactive oxygen species (ROS), like bleomycin, H2O2 and phenazine methosulfate. NPM1 stimulates endodeoxyribonuclease activity on double-stranded DNA with AP sites, but inhibits endoribonuclease activity on single-stranded RNA containing AP sites. Belongs to the DNA repair enzymes AP/ExoA family.

Protein type: DNA-binding; EC 4.2.99.18; Nuclear receptor co-regulator; Deoxyribonuclease; Nucleolus; DNA repair, damage; Hydrolase; Endoplasmic reticulum; Lyase; Transcription, coactivator/corepressor

Chromosomal Location of Human Ortholog: 14q11.2

Cellular Component: nucleoplasm; centrosome; transcription factor complex; mitochondrion; endoplasmic reticulum; perinuclear region of cytoplasm; cytoplasm; nucleolus; ribosome; nuclear speck; nucleus

Molecular Function: phosphodiesterase I activity; DNA-(apurinic or apyrimidinic site) lyase activity; phosphoric diester hydrolase activity; uracil DNA N-glycosylase activity; chromatin DNA binding; metal ion binding; transcription coactivator activity; oxidoreductase activity; endodeoxyribonuclease activity; NF-kappaB binding; protein binding; DNA binding; endonuclease activity; protein complex binding; damaged DNA binding; ribonuclease H activity; 3'-5' exonuclease activity; transcription corepressor activity; double-stranded DNA specific 3'-5' exodeoxyribonuclease activity; site-specific endodeoxyribonuclease activity, specific for altered base

Biological Process: response to drug; positive regulation of DNA repair; mismatch repair; transcription, DNA-dependent; negative regulation of smooth muscle cell migration; DNA strand elongation during DNA replication; DNA repair; regulation of mRNA stability; DNA catabolic process, endonucleolytic; double-strand break repair via homologous recombination; telomere maintenance via semi-conservative replication; regulation of transcription, DNA-dependent; cell redox homeostasis; nucleotide-excision repair; base-excision repair; transcription-coupled nucleotide-excision repair; double-strand break repair; telomere maintenance via recombination; nucleotide-excision repair, DNA gap filling; mitotic cell cycle; DNA catabolic process, exonucleolytic; telomere maintenance; aging