Typical Testing Data/Standard Curve (for reference only)
General Car ELISA Kit | Car elisa kit
Car (Carnosine) ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Car. During the reaction, Car in the sample or standard competes with a fixed amount of Car on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Car. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) are added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of Car in the samples is then determined by comparing the OD of the samples to the standard curve.
NCBI and Uniprot Product Information
Uniprot Description
TRIM13: E3 ubiquitin ligase involved in the retrotranslocation and turnover of membrane and secretory proteins from the ER through a set of processes named ER-associated degradation (ERAD). This process acts on misfolded proteins as well as in the regulated degradation of correctly folded proteins. Enhances ionizing radiation-induced p53/TP53 stability and apoptosis via ubiquitinating MDM2 and AKT1 and decreasing AKT1 kinase activity through MDM2 and AKT1 proteasomal degradation. Regulates ER stress-induced autophagy, and may act as a tumor suppressor. Belongs to the TRIM/RBCC family. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Tumor suppressor; Ubiquitin ligase; Ligase; EC 6.3.2.-; Membrane protein, integral; Ubiquitin conjugating system
Chromosomal Location of Human Ortholog: 13q14
Cellular Component: endoplasmic reticulum membrane; cytoplasm; integral to membrane
Molecular Function: protein binding; signal transducer activity; zinc ion binding; ubiquitin-protein ligase activity; ligase activity
Biological Process: proteasomal ubiquitin-dependent protein catabolic process; negative regulation of viral transcription; protein autoubiquitination; ER-associated protein catabolic process; anatomical structure morphogenesis; positive regulation of I-kappaB kinase/NF-kappaB cascade; response to gamma radiation; innate immune response; signal transduction; positive regulation of macroautophagy; activation of NF-kappaB transcription factor
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Product Notes
The General Car trim13 (Catalog #AAA2516148) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2516148 ELISA Kit recognizes General Car. It is sometimes possible for the material contained within the vial of "Car, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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