Dehydroepiandrosterone (DHEA) DIY ELISA Kit | DHEA diy elisa kit

General Dehydroepiandrosterone (DHEA) ELISA Kit DIY Materials

Cat. Num. AAA2088547

• Gene Names: SULT2A1; HST; ST2; STD; hSTa; DHEAS; ST2A1; ST2A3; DHEA-ST
• Reactivity: Pan-species (General)
• Synonyms: Dehydroepiandrosterone (DHEA); General Dehydroepiandrosterone (DHEA) ELISA Kit DIY Materials; DHEA diy elisa kit

• Reactivity: Pan-species (General)
• Specificity: The Abs in the kit have high sensitivity and excellent specificity for detection of Dehydroepiandrosterone (DHEA). No significant cross-reactivity or interference between Dehydroepiandrosterone (DHEA) and analogues was observed.
• Sequence Length: 285

• Application Notes: Main materials for "Do It Yourself ELISA Kit"
• Assay Type: Competitive Inhibition ELISA for Antigen Detection
• Samples: Serum, plasma and other biological fluids
• Detection Range: 123.46-10000pg/mL
• Sensitivity: 256pg/mL
• Reagent Contents: Capture Antibody, Biontin-labeled Competitor, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
• Product Usage: 1. Coat the plates with 100muL per well of working solution of Capture Antibody.incubate overnight at 4 degree C or incubate at 37 degree C for 2 hours.; 2. Aspirate and wash 1 time.; 3. Block the plates with 200 muL per well of working solution of Blocking Buffer. Incubate at 37 degree C for 1.5 hours.; 4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.
• Other Reagents Required: ELISA/CLIA Support Pack 2. (Catalog MBS2089472)
• Preparation and Storage: Antibodies, Standard and Streptavidin-HRP should be stored at -20 degree C. TMB should be stored at 4 degree C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4 degree C.

Product Categories/Family for DHEA diy elisa kit

Endocrinology; Reproductive science; Hormone metabolism; Rheumatology

NCBI and Uniprot Product Information

• NCBI GI #: 908768
• NCBI GeneID: 6822
• Molecular Weight: 33,780 Da
• NCBI Official Full Name: dehydroepiandrosterone sulfotransferase
• NCBI Official Synonym Full Names: sulfotransferase family 2A member 1
• NCBI Official Symbol: SULT2A1
• NCBI Official Synonym Symbols: HST; ST2; STD; hSTa; DHEAS; ST2A1; ST2A3; DHEA-ST
• NCBI Protein Information: bile salt sulfotransferase
• UniProt Protein Name: Bile salt sulfotransferase
• UniProt Gene Name: SULT2A1
• UniProt Synonym Gene Names: HST; STD; DHEA-ST; HST; ST2A1

NCBI Description

This gene encodes a member of the sulfotransferase family. Sulfotransferases aid in the metabolism of drugs and endogenous compounds by converting these substances into more hydrophilic water-soluble sulfate conjugates that can be easily excreted. This protein catalyzes the sulfation of steroids and bile acids in the liver and adrenal glands, and may have a role in the inherited adrenal androgen excess in women with polycystic ovary syndrome. [provided by RefSeq, Mar 2010]

Uniprot Description

SULT2A1: Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) as sulfonate donor to catalyze the sulfonation of steroids and bile acids in the liver and adrenal glands. Belongs to the sulfotransferase 1 family.

Protein type: EC 2.8.2.14; Transferase

Chromosomal Location of Human Ortholog: 19q13.33

Cellular Component: cytoplasm; cytosol

Molecular Function: aryl sulfotransferase activity; estrone sulfotransferase activity; protein binding; steroid sulfotransferase activity; sulfotransferase activity

Biological Process: 3'-phosphoadenosine 5'-phosphosulfate metabolic process; steroid metabolic process; sulfation