Typical Testing Data/Standard Curve (for reference only)
General Nitrate Reductase Assay Kit | Marky_1807 assay kit
Nitrate Reductase Assay Kit
Reactivity
General
Applications
Functional Assay
Synonyms
Nitrate Reductase; Nitrate Reductase Assay Kit; Marky_1807 assay kit
Reactivity
General
Sequence Length
943
Applicable Applications for Marky_1807 assay kit
Functional Assay
Samples
Tissue extracts, cell lysate samples
Detection Range
0.1 umol/ml - 8 umol/ml
Preparation and Storage
Shipped and store at 4 degree C for 6 months.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for Marky_1807 assay kit
Background/Introduction: Nitrate Reductase (NR), also known as respiratory nitrate reductase. Eukaryotic nitrate reductases are part of the sulfite oxidase family of molybdoenzymes. They transfer electrons from NADH or NADPH to nitrate. Prokaryotic nitrate reductases belong to the DMSO reductase family of molybdoenzymes and have been classified into three groups, assimilatory nitrate reductases (Nas), respiratory nitrate reductase (Nar), and periplasmic nitrate reductases (Nap). The active site of these enzymes is a Mo ion that is bound to the four thiolate functions of two pterin molecules. The coordination sphere of the Mo is completed by one amino-acid side chain and oxygen and/or sulfur ligands. The exact environment of the Mo ion in certain of these enzymes (oxygen versus sulfur as a sixth molybdenum ligand) is still debated. The Mo is covalently attached to the protein by a cysteine ligand in Nap, and an aspartate in Nar. Nitrate reductase activity can be used as a biochemical tool for predicting grain yield and grain protein production. Nitrate reductase promotes amino acid production in tea leaves. It is reported that tea plants sprayed with various micronutrients (like Zn, Mn and B) along with Mo enhanced the amino acid content of tea shoots and also the crop yield. The assay is initiated with the enzymatic hydrolysis of the nitrate by Nitrate Reductase. The enzyme catalysed reaction products azo-compound can be measured at a colorimetric readout at 540 nm.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
106,991 Da
NCBI Official Full Name
nitrate reductase
NCBI Official Symbol
Marky_1807
NCBI Protein Information
nitrate reductase
UniProt Protein Name
Nitrate reductase
Protein Family
UniProt Gene Name
Marky_1807
UniProt Entry Name
F2NPN8_MARHT
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Product Notes
The General Marky_1807 marky_1807 (Catalog #AAA822350) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA822350 Assay Kit recognizes General Marky_1807. AAA Biotech's Nitrate Reductase can be used in a range of immunoassay formats including, but not limited to, Functional Assay. Researchers should empirically determine the suitability of the Marky_1807 marky_1807 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Nitrate Reductase, Assay Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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