General Ceruloplasmin Assay Kit | CP assay kit

Ceruloplasmin Microplate Assay Kit

Cat. Num. AAA8243193

• Gene Names: CP; CP-2
• Reactivity: General
• Applications: Functional Assay
• Synonyms: Ceruloplasmin; Ceruloplasmin Microplate Assay Kit; EC 1.16.3.1; Ferroxidase; HEPH; Hephaestin; CP assay kit

• Reactivity: General
• Sequence Length: 1065

• Applicable Applications for CP assay kit: Functional Assay
• Samples: Serum, Plasma, Other biological fluids Samples.
• Preparation and Storage: Shipped and store at 4 degree C for 6 months.

Related Product Information for CP assay kit

Background/Introduction: Ceruloplasmin is a copper containing protein found primarily in the blood. It carries approximately 70% of the total copper present in the blood. Ceruloplasmin exhibits a weak oxidase activity, which is a more accurate method of assessing ceruloplasmin in serum than immunodiffusion or other non-enzymatic assays. Normal Ceruloplasmin levels are generally 1-4 uM (15-60 mg/dl), equivalent to approximately 50-150 mU/ml. Elevated amounts of serum ceruloplasmin are found in pregnancy, cancer, rheumatoid arthritis and in several mental conditions such as Alzheimer's, schizophrenia and OCD. Abnormally low amounts are found in Wilson's and Menkes' Diseases and in several other rare conditions. The assay is initiated with the enzymatic catalysis of the TMB by CP. The enzyme catalysed reaction products can be measured at a colorimetric readout at 645 nm.

NCBI and Uniprot Product Information

• NCBI GI #: 4557485
• NCBI GeneID: 1356
• NCBI Accession #: NP_000087.1
• NCBI GenBank Nucleotide #: NM_000096.3
• Molecular Weight: 122,205 Da
• NCBI Official Full Name: ceruloplasmin
• NCBI Official Synonym Full Names: ceruloplasmin (ferroxidase)
• NCBI Official Symbol: CP
• NCBI Official Synonym Symbols: CP-2
• NCBI Protein Information: ceruloplasmin
• UniProt Protein Name: Ceruloplasmin
• Protein Family: Ceruloplasmin
• UniProt Gene Name: CP
• UniProt Entry Name: CERU_HUMAN

NCBI Description

The protein encoded by this gene is a metalloprotein that binds most of the copper in plasma and is involved in the peroxidation of Fe(II)transferrin to Fe(III) transferrin. Mutations in this gene cause aceruloplasminemia, which results in iron accumulation and tissue damage, and is associated with diabetes and neurologic abnormalities. Two transcript variants, one protein-coding and the other not protein-coding, have been found for this gene. [provided by RefSeq, Feb 2012]

Uniprot Description

CP: Ceruloplasmin is a blue, copper-binding (6-7 atoms per molecule) glycoprotein. It has ferroxidase activity oxidizing Fe(2+) to Fe(3+) without releasing radical oxygen species. It is involved in iron transport across the cell membrane. Provides Cu(2+) ions for the ascorbate-mediated deaminase degradation of the heparan sulfate chains of GPC1. May also play a role in fetal lung development or pulmonary antioxidant defense. Defects in CP are the cause of aceruloplasminemia (ACERULOP). It is an autosomal recessive disorder of iron metabolism characterized by iron accumulation in the brain as well as visceral organs. Clinical features consist of the triad of retinal degeneration, diabetes mellitus and neurological disturbances. Ceruloplasmin levels are decreased in Wilson disease, in which copper cannot be incorporated into ceruloplasmin in liver because of defects in the copper-transporting ATPase 2. Belongs to the multicopper oxidase family.

Protein type: Oxidoreductase; Secreted, signal peptide; EC 1.16.3.1; Cofactor and Vitamin Metabolism - porphyrin and chlorophyll; Secreted

Chromosomal Location of Human Ortholog: 3q23-q25

Cellular Component: anchored to plasma membrane; extracellular region; extracellular space; lysosomal membrane

Molecular Function: chaperone binding; copper ion binding; ferroxidase activity

Biological Process: aging; cellular iron ion homeostasis; copper ion transport; liver development; lung development; response to copper ion; response to nutrient; transmembrane transport

Disease: Aceruloplasminemia