Typical Testing Data/Standard Curve (for reference only)
Bovine Gelsolin ELISA Kit | Gel elisa kit
Bovine Gelsolin ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the assay: GSN ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-GSN antibody and an GSN-HRP conjugate. The assay sample and buffer are incubated together with GSN-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the GSN concentration since GSN from samples and GSN-HRP conjugate compete for the anti-GSN antibody binding site. Since the number of sites is limited, as more sites are occupied by GSN from the sample, fewer sites are left to bind GSN-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The GSN concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
plays a role in phosphoinositide mediated disassembly of actin filaments in Sertoli cell adhesion complexes; may regulate sperm release and turnover of the blood-testis barrier [RGD, Feb 2006]
Uniprot Description
Gelsolin: a calcium-regulated, actin-modulating protein that binds to the plus (or barbed) ends of actin monomers or filaments, preventing monomer exchange (end-blocking or capping). It can promote the assembly of monomers into filaments (nucleation) as well as sever filaments already formed. Its expression is significantly decreased in many tumors and transformed cell lines. Gelsolin and other proteins including E-cadherin, BRCA, p21WAF, HoxA5, and retinoic acid receptor II are regulated by epigenetic changes in breast cancers. Gelsolin helps mediate the ability of HDAC inhibitors to protect neurons from oxygen/glucose deprivation. Two human isoforms are produced by alternative initiation.
Protein type: Secreted, signal peptide; Secreted; Motility/polarity/chemotaxis; Nuclear receptor co-regulator; Actin-binding
Cellular Component: ruffle; extracellular space; focal adhesion; protein complex; perinuclear region of cytoplasm; lamellipodium; extracellular region; cytosol; actin cytoskeleton
Molecular Function: calcium ion binding; actin binding
Biological Process: regulation of cell adhesion; actin filament severing; phosphoinositide-mediated signaling; wound healing; apoptosis; actin nucleation; vesicle-mediated transport; response to ethanol; actin filament polymerization; tissue regeneration; actin polymerization and/or depolymerization; response to folic acid; oligodendrocyte development; barbed-end actin filament capping; aging
Research Articles on Gel
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Product Notes
The Bovine Gel gsn (Catalog #AAA7201201) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA7201201 ELISA Kit recognizes Bovine Gel. It is sometimes possible for the material contained within the vial of "Gelsolin, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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