Human Growth Differentiation Factor 6 ELISA Kit | GDF6 elisa kit

Human Growth Differentiation Factor 6 ELISA Kit

Cat. Num. AAA763810

• Gene Names: GDF6; KFM; KFS; KFS1; KFSL; SGM1; BMP13; CDMP2; LCA17; MCOP4; SCDO4; BMP-13; MCOPCB6
• Reactivity: Human
• Synonyms: Growth Differentiation Factor 6; Human Growth Differentiation Factor 6 ELISA Kit; GDF6/BMP-13/BMP13/CDMP2/GDF16/GDF-6/growth differentiation factor 6/Growth/differentiation factor 16/growth/differentiation factor 6/KFS/KFS1/KFSL/MCOP4/MCOPCB6/MGC158100/MGC158101/SCDO4/SGM1; GDF6 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of GDF6. No significant cross-reactivity or interference between GDF6 and analogues was observed.
• Sequence Length: 455

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 0.156-10ng/ml
• Sensitivity: 0.094ng/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level GDF6 were tested 20 times on one plate, respectively. Intra-Assay: CV<8%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level GDF6 were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for GDF6 elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- GDF6 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- GDF6 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the GDF6 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of GDF6 can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 119612153
• NCBI GeneID: 392255
• Molecular Weight: 50,662 Da
• NCBI Official Full Name: growth differentiation factor 6, partial
• NCBI Official Synonym Full Names: growth differentiation factor 6
• NCBI Official Symbol: GDF6
• NCBI Official Synonym Symbols: KFM; KFS; KFS1; KFSL; SGM1; BMP13; CDMP2; LCA17; MCOP4; SCDO4; BMP-13; MCOPCB6
• NCBI Protein Information: growth/differentiation factor 6
• UniProt Protein Name: Growth/differentiation factor 6
• Protein Family: Growth/differentiation factor
• UniProt Gene Name: GDF6
• UniProt Synonym Gene Names: BMP13; GDF16; GDF-6; BMP-13
• UniProt Entry Name: GDF6_HUMAN

NCBI Description

This gene encodes a member of the bone morphogenetic protein (BMP) family and the TGF-beta superfamily of secreted signaling molecules. It is required for normal formation of some bones and joints in the limbs, skull, and axial skeleton. Mutations in this gene result in colobomata, which are congenital abnormalities in ocular development, and in Klippel-Feil syndrome (KFS), which is a congenital disorder of spinal segmentation. [provided by RefSeq, Jul 2008]

Uniprot Description

GDF6: Required for normal formation of bones and joints in the limbs, skull, and axial skeleton. Plays a key role in establishing boundaries between skeletal elements during development. Defects in GDF6 are the cause of Klippel-Feil syndrome type 1 (KFS1). A skeletal disorder characterized by congenital fusion of cervical vertebrae. It is due to a failure in the normal segmentation of vertebrae during the early weeks of fetal development. The clinical triad consists of short neck, low posterior hairline, and limited neck movement. Deafness is a well- known feature of KFS and may be of sensorineural, conductive, or mixed type. A chromosomal aberration involving GDF6 has been found in a patient with Klippel-Feil syndrome (KFS). Paracentric inv(8)(q22;2q23.3). Defects in GDF6 are the cause of microphthalmia isolated type 4 (MCOP4). A disorder of eye formation, ranging from small size of a single eye to complete bilateral absence of ocular tissues. Ocular abnormalities like opacities of the cornea and lens, scaring of the retina and choroid, cataract and other abnormalities like cataract may also be present. Belongs to the TGF-beta family.

Protein type: Secreted, signal peptide; Secreted; Cell development/differentiation; Cytokine

Chromosomal Location of Human Ortholog: 8q22.1

Cellular Component: extracellular space

Molecular Function: cytokine activity; growth factor activity; protein homodimerization activity; transforming growth factor beta receptor binding

Biological Process: activin receptor signaling pathway; apoptosis; BMP signaling pathway; cell development; growth; positive regulation of neuron differentiation; positive regulation of transcription, DNA-dependent; regulation of apoptosis; regulation of MAPKKK cascade

Disease: Klippel-feil Syndrome 1, Autosomal Dominant; Leber Congenital Amaurosis 17; Microphthalmia, Isolated 4; Microphthalmia, Isolated, With Coloboma 6