Growth Differentiation Factor 5 (GDF5) Recombinant Protein | GDF5 recombinant protein

Recombinant Growth Differentiation Factor 5 (GDF5)

Cat. Num. AAA2010117

• Gene Names: GDF5; OS5; LAP4; BDA1C; BMP14; CDMP1; SYM1B; SYNS2
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Growth Differentiation Factor 5 (GDF5); Recombinant Growth Differentiation Factor 5 (GDF5); GDF5 recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with N-terminal His-Tag, its sequence is listed below.; APLATRQGK RPSKNLKARC SRKALHVNFK DMGWDDWIIA PLEYEAFHCE GLCEFPLRSH LEPTNHAVIQ TLMNSMDPES TPPTCCVPTR LSPISILFID SANNVVYKQY EDMVVESCGC R
• Sequence Length: 501

• Applicable Applications for GDF5 recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Homo sapiens (Human)
• Expression System: Prokaryotic expression
• Residues: Ala381~Arg501(Accession # P43026) with N-terminal His-Tag
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

Sequence Information

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 4503969
• NCBI GeneID: 8200
• NCBI Accession #: NP_000548.1
• NCBI GenBank Nucleotide #: NM_000557.3
• UniProt Accession #: P43026; Q96SB1; E1P5Q2
• Molecular Weight: 16kDa
• NCBI Official Full Name: growth/differentiation factor 5 preproprotein
• NCBI Official Synonym Full Names: growth differentiation factor 5
• NCBI Official Symbol: GDF5
• NCBI Official Synonym Symbols: OS5; LAP4; BDA1C; BMP14; CDMP1; SYM1B; SYNS2
• NCBI Protein Information: growth/differentiation factor 5; GDF-5; CDMP-1; radotermin; cartilage-derived morphogenetic protein-1
• UniProt Protein Name: Growth/differentiation factor 5
• Protein Family: Growth/differentiation factor
• UniProt Gene Name: GDF5
• UniProt Synonym Gene Names: CDMP1; GDF-5; CDMP-1
• UniProt Entry Name: GDF5_HUMAN

NCBI Description

The protein encoded by this gene is a member of the bone morphogenetic protein (BMP) family and the TGF-beta superfamily. This group of proteins is characterized by a polybasic proteolytic processing site which is cleaved to produce a mature protein containing seven conserved cysteine residues. The members of this family are regulators of cell growth and differentiation in both embryonic and adult tissues. Mutations in this gene are associated with acromesomelic dysplasia, Hunter-Thompson type; brachydactyly, type C; and chondrodysplasia, Grebe type. These associations confirm that the gene product plays a role in skeletal development. [provided by RefSeq, Jul 2008]

Uniprot Description

GDF5: a cytokine that is a member of the bone morphogenetic protein (BMP) family and the TGF-beta superfamily. These cytokines are characterized by a polybasic proteolytic processing site which is cleaved to produce a mature protein containing seven conserved cysteine residues. The members of this family are regulators of cell growth and differentiation in both embryonic and adult tissues. Binds to bone morphogenetic protein receptors (BMPRs), a family of transmembrane serine/threonine kinases. BMPRs are involved in bone and cartilage formation. Chondrogenic signaling is mediated by the high-affinity receptor BMPR1B. Defects in GDF5 are the cause of acromesomelic chondrodysplasia Grebe type (AMDG), acromesomelic chondrodysplasia Hunter-Thompson type (AMDH), brachydactyly type C (BDC), Du Pan syndrome (DPS), symphalangism proximal syndrome (SYM1), multiple synostoses syndrome type 2 (SYNS2), and brachydactyly type A2 (BDA2). Genetic variations in GDF5 are associated with susceptibility to osteoarthritis type 5 (OS5). Belongs to the TGF-beta family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 20q11.2

Cellular Component: extracellular space; extracellular region; plasma membrane

Molecular Function: identical protein binding; protein binding; growth factor activity; cytokine activity; transforming growth factor beta receptor binding

Biological Process: hindlimb morphogenesis; extracellular matrix organization and biogenesis; regulation of multicellular organism growth; forelimb morphogenesis; BMP signaling pathway; regulation of apoptosis; positive regulation of chondrocyte differentiation; cell-cell signaling; transforming growth factor beta receptor signaling pathway; regulation of MAPKKK cascade; chondrocyte differentiation; negative regulation of neuron apoptosis; positive regulation of neuron differentiation; cell development; embryonic limb morphogenesis; negative regulation of epithelial cell proliferation; growth

Disease: Chondrodysplasia, Grebe Type; Acromesomelic Dysplasia, Hunter-thompson Type; Brachydactyly, Type A1, C; Brachydactyly, Type A2; Symphalangism, Proximal, 1b; Brachydactyly, Type C; Fibular Hypoplasia And Complex Brachydactyly; Osteoarthritis Susceptibility 5; Multiple Synostoses Syndrome 2