Typical Testing Data/Standard Curve (for reference only)
Canine Granulocyte Chemotactic Protein 2 ELISA Kit | GCP2 elisa kit
Canine Granulocyte Chemotactic Protein 2 ELISA Kit
Gene Names
TUBGCP2; GCP2; GCP-2; h103p; hGCP2; SPBC97; Spc97p; hSpc97; Grip103
Reactivity
Canine
Synonyms
Granulocyte Chemotactic Protein 2; Canine Granulocyte Chemotactic Protein 2 ELISA Kit; GCP2 elisa kit
Reactivity
Canine
Samples
Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Assay Type
Sandwich
Detection Range
25-500pg/mL
Sensitivity
1.0pg/mL
Intended Uses
This GCP2 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Canine GCP2. This ELISA kit for research use only, not for therapeutic applications!
Preparation and Storage
Store all reagents at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for GCP2 elisa kit
Principle of the Assay: GCP2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-GCP2 antibody and an GCP2-HRP conjugate. The assay sample and buffer are incubated together with GCP2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the GCP2 concentration since GCP2 from samples and GCP2-HRP conjugate compete for the anti-GCP2 antibody binding site. Since the number of sites is limited, as more sites are occupied by GCP2 from the sample, fewer sites are left to bind GCP2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The GCP2 concentration in each sample is interpolated from this standard curve.
Product Categories/Family for GCP2 elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
105,628 Da
NCBI Official Full Name
gamma-tubulin complex component 2 isoform 2
NCBI Official Synonym Full Names
tubulin, gamma complex associated protein 2
NCBI Official Symbol
TUBGCP2
NCBI Official Synonym Symbols
GCP2; GCP-2; h103p; hGCP2; SPBC97; Spc97p; hSpc97; Grip103
NCBI Protein Information
gamma-tubulin complex component 2; hGrip103; gamma-ring complex protein 103 kDa; spindle pole body protein Spc97 homolog
UniProt Protein Name
Gamma-tubulin complex component 2
Protein Family
UniProt Gene Name
TUBGCP2
UniProt Synonym Gene Names
GCP2; GCP-2; hGCP2; h103p; hGrip103; hSpc97
UniProt Entry Name
GCP2_HUMAN
Uniprot Description
GCP2: the gamma-tubulin complex, composed of gamma-tubulin, GCP2, GCP3, GCP4, GCP5 and GCP6, is necessary for microtubule nucleation at the centrosome.
Protein type: Cytoskeletal; Motility/polarity/chemotaxis
Chromosomal Location of Human Ortholog: 10q26.3
Cellular Component: nucleoplasm; spindle pole; centrosome; membrane; cytoplasmic microtubule; microtubule organizing center; cytosol
Molecular Function: protein binding
Biological Process: protein complex assembly; mitotic cell cycle; G2/M transition of mitotic cell cycle; microtubule nucleation
Research Articles on GCP2
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Product Notes
The Canine GCP2 tubgcp2 (Catalog #AAA740107) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA740107 ELISA Kit recognizes Canine GCP2. It is sometimes possible for the material contained within the vial of "Granulocyte Chemotactic Protein 2, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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