Human Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1 ELISA Kit | GBF1 elisa kit

Human Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1 (GBF1) ELISA Kit

Cat. Num. AAA280968

• Gene Names: GBF1; ARF1GEF
• Reactivity: Human
• Synonyms: Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1; Human Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1 (GBF1) ELISA Kit; GBF1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Human GBF1. No significant cross-reactivity or interference between Human GBF1 and analogues was observed.
• Sequence Length: 1859

• Samples: Serum, Plasma, Other biological fluids
• Assay Type: Sandwich
• Sample Volume: 1-200 uL
• Precision: Intra-assay Precision (Precision within an assay); Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.; Inter-assay Precision (Precision between assays); Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.; CV (%) = SD/meanX100; Intra-Assay: CV; Inter-Assay: CV
• Detection Wavelength: 450 nm
• Preparation and Storage: Store at 2-8 degree C.

Related Product Information for GBF1 elisa kit

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate GBF1 in samples. An antibody specific for GBF1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyGBF1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for GBF1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of GBF1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 313747582
• NCBI GeneID: 8729
• NCBI Accession #: NP_001186307.1
• NCBI GenBank Nucleotide #: NM_001199378.1
• UniProt Accession #: Q92538; Q149P0; Q149P1; Q5VXX3; Q96CK6; Q96HZ3; Q9H473
• Molecular Weight: 206,016 Da
• NCBI Official Full Name: Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1 isoform 2
• NCBI Official Synonym Full Names: golgi brefeldin A resistant guanine nucleotide exchange factor 1
• NCBI Official Symbol: GBF1
• NCBI Official Synonym Symbols: ARF1GEF
• NCBI Protein Information: Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1
• UniProt Protein Name: Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1
• Protein Family: G-box-binding factor
• UniProt Gene Name: GBF1
• UniProt Synonym Gene Names: KIAA0248; BFA-resistant GEF 1

NCBI Description

This gene encodes a member of the Sec7 domain family. The encoded protein is a guanine nucleotide exchange factor that regulates the recruitment of proteins to membranes by mediating GDP to GTP exchange. The encoded protein is localized to the Golgi apparatus and plays a role in vesicular trafficking by activating ADP ribosylation factor 1. The encoded protein has also been identified as an important host factor for viral replication. Multiple transcript variants have been observed for this gene. [provided by RefSeq, Dec 2010]

Uniprot Description

Guanine-nucleotide exchange factor (GEF) for members of the Arf family of small GTPases involved in trafficking in the early secretory pathway; its GEF activity initiates the coating of nascent vesicles via the localized generation of activated ARFs through replacement of GDP with GTP. Recruitment to cis-Golgi membranes requires membrane association of Arf-GDP and can be regulated by ARF1, ARF3, ARF4 and ARF5. Involved in the recruitment of the COPI coat complex to the endoplasmic reticulum exit sites (ERES), and the endoplasmic reticulum-Golgi intermediate (ERGIC) and cis-Golgi compartments which implicates ARF1 activation. Involved in COPI vesicle-dependent retrograde transport from the ERGIC and cis-Golgi compartments to the endoplasmic reticulum (ER) (PubMed:16926190, PubMed:17956946, PubMed:18003980, PubMed:12047556, PubMed:12808027, PubMed:19039328, PubMed:24213530). Involved in the trans-Golgi network recruitment of GGA1, GGA2, GGA3, BIG1, BIG2, and the AP-1 adaptor protein complex related to chlathrin-dependent transport; the function requires its GEF activity (probably at least in part on ARF4 and ARF5) (PubMed:23386609). Has GEF activity towards ARF1 (PubMed:15616190). Has in vitro GEF activity towards ARF5 (). Involved in the processing of PSAP (PubMed:17666033). Required for the assembly of the Golgi apparatus (PubMed:12808027, PubMed:18003980). The AMPK-phosphorylated form is involved in Golgi disassembly during mitotis and under stress conditions (PubMed:18063581, PubMed:23418352). May be involved in the COPI vesicle-dependent recruitment of PNPLA2 to lipid droplets; however, this function is under debate (PubMed:19461073, PubMed:22185782). In neutrophils, involved in G protein-coupled receptor (GPCR)-mediated chemotaxis und superoxide production. Proposed to be recruited by phosphatidylinositol-phosphates generated upon GPCR stimulation to the leading edge where it recruits and activates ARF1, and is involved in recruitment of GIT2 and the NADPH oxidase complex (PubMed:22573891).