Human GATA Binding Protein 5 ELISA Kit | GATA5 elisa kit

Human GATA Binding Protein 5 ELISA Kit

Cat. Num. AAA721355

• Gene Names: GATA5; GATAS; bB379O24.1
• Reactivity: Human
• Synonyms: GATA Binding Protein 5; Human GATA Binding Protein 5 ELISA Kit; GATA5 elisa kit

• Reactivity: Human

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive or Sandwich
• Detection Range: 1.0-25ng/mL
• Sensitivity: 0.1ng/mL
• Intended Uses: This GATA5 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human GATA5. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for GATA5 elisa kit

Principle of the Assay: GATA5 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for GATA5. Standards or samples are then added to the microtiter plate wells and GATA5 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of GATA5 present in the sample, a standardized preparation of horseradish peroxidase (HRP) -conjugated polyclonal antibody, specific for GATA5 are added to each well to "sandwich" the GATA5 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain GATA5 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The GATA5 concentration in each sample is interpolated from this standard curve.

Product Categories/Family for GATA5 elisa kit

Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 29126838
• NCBI GeneID: 140628
• Molecular Weight: 41,299 Da
• NCBI Official Full Name: GATA5 protein, partial
• NCBI Official Synonym Full Names: GATA binding protein 5
• NCBI Official Symbol: GATA5
• NCBI Official Synonym Symbols: GATAS; bB379O24.1
• NCBI Protein Information: transcription factor GATA-5; GATA binding factor-5; GATA-binding factor 5; GATA-binding protein 5
• UniProt Protein Name: Transcription factor GATA-5
• Protein Family: GATA transcription factor
• UniProt Gene Name: GATA5
• UniProt Entry Name: GATA5_HUMAN

NCBI Description

The protein encoded by this gene is a transcription factor that contains two GATA-type zinc fingers. The encoded protein is known to bind to hepatocyte nuclear factor-1alpha (HNF-1alpha), and this interaction is essential for cooperative activation of the intestinal lactase-phlorizin hydrolase promoter. In other organisms, similar proteins may be involved in the establishment of cardiac smooth muscle cell diversity. [provided by RefSeq, Jul 2008]

Uniprot Description

GATA5: Binds to the functionally important CEF-1 nuclear protein binding site in the cardiac-specific slow/cardiac troponin C transcriptional enhancer. May play an important role in the transcriptional program(s) that underlies smooth muscle cell diversity. Rare variants in GATA5 may be a cause of susceptibility to atrial fibrillation, a common sustained cardiac rhythm disturbance. Atrial fibrillation is characterized by disorganized atrial electrical activity and ineffective atrial contraction promoting blood stasis in the atria and reduces ventricular filling. It can result in palpitations, syncope, thromboembolic stroke, and congestive heart failure.

Chromosomal Location of Human Ortholog: 20q13.33

Cellular Component: nucleoplasm; nucleus

Molecular Function: RNA polymerase II transcription factor activity, enhancer binding; zinc ion binding; transcription factor activity

Biological Process: transcription from RNA polymerase II promoter; positive regulation of transcription from RNA polymerase II promoter; blood coagulation