Bovine GATA Binding Protein 1 ELISA Kit | GATA1 elisa kit

Bovine GATA Binding Protein 1 ELISA Kit

Cat. Num. AAA745646

• Gene Names: GATA1; GF1; GF-1; NFE1; XLTT; ERYF1; NF-E1; XLANP; XLTDA; GATA-1
• Reactivity: Bovine
• Synonyms: GATA Binding Protein 1; Bovine GATA Binding Protein 1 ELISA Kit; GATA1 elisa kit

• Reactivity: Bovine

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Sensitivity: 0.1 ng/mL.
• Intended Uses: This GATA1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Bovine GATA1. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for GATA1 elisa kit

Principle of the assay: GATA1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-GATA1 antibody and an GATA1-HRP conjugate. The assay sample and buffer are incubated together with GATA1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the GATA1 concentration since GATA1 from samples and GATA1-HRP conjugate compete for the anti-GATA1 antibody binding site. Since the number of sites is limited, as more sites are occupied by GATA1 from the sample, fewer sites are left to bind GATA1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The GATA1 concentration in each sample is interpolated from this standard curve.

Product Categories/Family for GATA1 elisa kit

Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 119571136
• NCBI GeneID: 2623
• Molecular Weight: 34,232 Da
• NCBI Official Full Name: GATA binding protein 1 (globin transcription factor 1), isoform CRA_c
• NCBI Official Synonym Full Names: GATA binding protein 1 (globin transcription factor 1)
• NCBI Official Symbol: GATA1
• NCBI Official Synonym Symbols: GF1; GF-1; NFE1; XLTT; ERYF1; NF-E1; XLANP; XLTDA; GATA-1
• NCBI Protein Information: erythroid transcription factor; GATA-binding factor 1; NF-E1 DNA-binding protein; transcription factor GATA1; nuclear factor, erythroid 1; globin transcription factor 1; erythroid transcription factor 1
• UniProt Protein Name: Erythroid transcription factor
• Protein Family: GATA transcription factor
• UniProt Gene Name: GATA1
• UniProt Synonym Gene Names: ERYF1; GF1; GATA-1; GF-1
• UniProt Entry Name: GATA1_HUMAN

NCBI Description

This gene encodes a protein which belongs to the GATA family of transcription factors. The protein plays an important role in erythroid development by regulating the switch of fetal hemoglobin to adult hemoglobin. Mutations in this gene have been associated with X-linked dyserythropoietic anemia and thrombocytopenia. [provided by RefSeq, Jul 2008]

Uniprot Description

GATA1: Transcriptional activator which probably serves as a general switch factor for erythroid development. It binds to DNA sites with the consensus sequence [AT]GATA[AG] within regulatory regions of globin genes and of other genes expressed in erythroid cells. May form homodimers or heterodimers with other isoforms. Interacts (via the N-terminal zinc finger) with ZFPM1. Interacts with GFI1B. Interacts with PIAS4; the interaction enhances sumoylation and represses the transactivational activity in a sumoylation-independent manner. Interacts with LMCD1. Interacts with CREBBP; the interaction stimulates acetylation and transcriptional activity in vivo. Interacts with EP300. Erythrocytes. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Transcription factor; DNA-binding

Chromosomal Location of Human Ortholog: Xp11.23

Cellular Component: nucleoplasm; transcription factor complex; transcriptional repressor complex; nucleus

Molecular Function: protein binding; DNA binding; zinc ion binding; sequence-specific DNA binding; p53 binding; chromatin DNA binding; transcription factor activity; DNA bending activity

Biological Process: transcription from RNA polymerase II promoter; erythrocyte development; embryonic hemopoiesis; megakaryocyte differentiation; in utero embryonic development; positive regulation of erythrocyte differentiation; male gonad development; eosinophil differentiation; negative regulation of transcription from RNA polymerase II promoter; negative regulation of cell proliferation; negative regulation of bone mineralization; positive regulation of peptidyl-tyrosine phosphorylation; cell-cell signaling; positive regulation of osteoblast proliferation; erythrocyte differentiation; positive regulation of transcription from RNA polymerase II promoter; platelet formation; blood coagulation; basophil differentiation; negative regulation of apoptosis

Disease: Thrombocytopenia With Beta-thalassemia, X-linked; Thrombocytopenia, X-linked, With Or Without Dyserythropoietic Anemia; Anemia, X-linked, With Or Without Neutropenia And/or Platelet Abnormalities; Down Syndrome