RGS10 recombinant protein

RGS10, 1-181aa, Human, His tag, E Coli

Cat. Num. AAA204243

• Applications: SDS-Page
• Purity: > 90% by SDS-PAGE
• Synonyms: RGS10; 1-181aa; Human; His tag; E Coli; Regulator of G-protein signaling 10; Regulator of G protein signaling 10; RGS10 recombinant protein

• Host: E Coli
• Purity/Purification: > 90% by SDS-PAGE
• Form/Format: Liquid. In 20mM Tris-HCl Buffer (pH 8.0) containing 1mM DTT, 10% glycerol, 0.1M NaCl
• Concentration: 1 mg/ml (determined by Bradford assay) (varies by lot)
• Sequence: MGSSHHHHHH SSGLVPRGSH MGSHMFNRAV SRLSRKRPPS DIHDSDGSSS SSHQSLKSTA KWAASLENLL EDPEGVKRFR EFLKKEFSEE NVLFWLACED FKKMQDKTQM QEKAKEIYMT FLSSKASSQV NVEGQSRLNE KILEEPHPLM FQKLQDQIFN LMKYDSYSRF LKSDLFLKHK RTEEEEEDLP DAQTAAKRAS RIYNT
• Sequence Length: 181

• Applicable Applications for RGS10 recombinant protein: SDS-PAGE
• Antigen Species: Human
• Tag Information: His-tag
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for RGS10 recombinant protein

Regulator of G protein signaling (RGS) family members are regulatory molecules that act as GTPase activating proteins (GAPs) for G alpha subunits of heterotrimeric G proteins. RGS proteins are able to deactivate G protein subunits of the Gi alpha, Go alpha and Gq alpha subtypes. They drive G proteins into their inactive GDP-bound forms. Regulator of G protein signaling 10 (RGS10) belongs to this family. All RGS proteins share a conserved 120-amino acid sequence termed the RGS domain. This protein associates specifically with the activated forms of the two related G-protein subunits, G-alphai3 and G-alphaz but fails to interact with the structurally and functionally distinct G-alpha subunits. RGS10 protein is localized in the nucleus. Recombinant human RGS10 protein, fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography techniques.

Product Categories/Family for RGS10 recombinant protein

Signal Transduction

References

Cali J J., et al. (1992) J Biol Chem. 267:24023-24027.; Simon M I., et al. (1991) Science. 252:802-808.

NCBI and Uniprot Product Information

• NCBI GI #: 52694755
• NCBI GeneID: 6001
• NCBI Accession #: NP_001005339
• NCBI GenBank Nucleotide #: NM_001005339.1
• UniProt Accession #: O43665; Q6IAZ6; Q96GN0; A8K408; B1AMR8
• Molecular Weight: 23.7 kDa (205aa), confirmed by MALDI-TOF
• NCBI Official Full Name: regulator of G-protein signaling 10 isoform a
• NCBI Official Synonym Full Names: regulator of G protein signaling 10
• NCBI Official Symbol: RGS10
• NCBI Protein Information: regulator of G-protein signaling 10
• UniProt Protein Name: Regulator of G-protein signaling 10
• Protein Family: Regulator of G-protein signaling
• UniProt Gene Name: RGS10
• UniProt Synonym Gene Names: RGS10

NCBI Description

Regulator of G protein signaling (RGS) family members are regulatory molecules that act as GTPase activating proteins (GAPs) for G alpha subunits of heterotrimeric G proteins. RGS proteins are able to deactivate G protein subunits of the Gi alpha, Go alpha and Gq alpha subtypes. They drive G proteins into their inactive GDP-bound forms. Regulator of G protein signaling 10 belongs to this family. All RGS proteins share a conserved 120-amino acid sequence termed the RGS domain. This protein associates specifically with the activated forms of the two related G-protein subunits, G-alphai3 and G-alphaz but fails to interact with the structurally and functionally distinct G-alpha subunits. Regulator of G protein signaling 10 protein is localized in the nucleus. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

Regulates G protein-coupled receptor signaling cascades, including signaling downstream of the muscarinic acetylcholine receptor CHRM2. Inhibits signal transduction by increasing the GTPase activity of G protein alpha subunits, thereby driving them into their inactive GDP-bound form (PubMed:8774883, PubMed:10608901, PubMed:9353196, PubMed:11443111, PubMed:18434541). Modulates the activity of potassium channels that are activated in response to CHRM2 signaling (PubMed:11443111). Activity on GNAZ is inhibited by palmitoylation of the G-protein (PubMed:9353196).