UDP-Glucose 4-Epimerase/GALE Recombinant Protein | GALE recombinant protein

Recombinant Human UDP-Glucose 4-Epimerase/GALE Protein (N-6His)

Cat. Num. AAA2553358

• Gene Names: GALE; SDR1E1
• Purity: >95% as determined by reducing SDS-PAGE.
• Synonyms: UDP-Glucose 4-Epimerase/GALE; Recombinant Human UDP-Glucose 4-Epimerase/GALE Protein (N-6His); UDP-Glucose 4-Epimerase; Galactowaldenase; UDP-Galactose 4-Epimerase; GALE; GALE recombinant protein

• Host: E Coli
• Purity/Purification: >95% as determined by reducing SDS-PAGE.
• Form/Format: Supplied as a 0.2 mum filtered solution of 50mM Tris HCl, 150mM NaCl, 2mM DTT, 1mM EDTA, pH 8.0.
• Sequence Positions: Met1-Ala348
• Sequence Length: 348

• Species: Human
• Endotoxin: <1.0 EU per ug as determined by LAL test.
• Fusion Tag: N-6His
• Preparation and Storage: Store at < -20 degree C, stable for 6 months after receipt. Please minimize freeze-thaw cycles.

Related Product Information for GALE recombinant protein

The enzyme UDP-Glucose 4-Epimerase (GALE) is a homodimeric epimerase found in bacterial, plant and mammalian cells. UDP-Glucose 4-Epimerase performs the final step in the Leloir pathway of Galactose metabolism, it catalyzes two distinct but analogous reactions: the epimerization of UDP-Gglucose to UDP-Galactose and the epimerization of UDP-N-Acetylglucosamine to UDP-N-Acetylgalactosamine. The bifunctional nature of the enzyme has the important metabolic consequence that mutant cells (or individuals) are dependent not only on exogenous galactose, but also on exogenous N-acetylgalactosamine as a necessary precursor for the synthesis of glycoproteins and glycolipids.

NCBI and Uniprot Product Information

• NCBI GI #: 56237023
• NCBI GeneID: 2582
• NCBI Accession #: NP_000394.2
• NCBI GenBank Nucleotide #: NM_000403.3
• UniProt Accession #: Q14376; Q38G75; Q86W41; Q9BVE3; Q9UJB4; A0A024RAH5; B3KQ39
• Molecular Weight: Molecular Mass: 40.4 kDa; Actual Protein Molecular Mass: 35 kDa
• NCBI Official Full Name: UDP-glucose 4-epimerase
• NCBI Official Synonym Full Names: UDP-galactose-4-epimerase
• NCBI Official Symbol: GALE
• NCBI Official Synonym Symbols: SDR1E1
• NCBI Protein Information: UDP-glucose 4-epimerase
• UniProt Protein Name: UDP-glucose 4-epimerase
• UniProt Gene Name: GALE
• UniProt Synonym Gene Names: UDP-GalNAc 4-epimerase1 PublicationManual assertion based on opinion iniRef.9; UDP-GlcNAc 4-epimerase1 PublicationManual assertion based on opinion iniRef.9

NCBI Description

This gene encodes UDP-galactose-4-epimerase which catalyzes two distinct but analogous reactions: the epimerization of UDP-glucose to UDP-galactose, and the epimerization of UDP-N-acetylglucosamine to UDP-N-acetylgalactosamine. The bifunctional nature of the enzyme has the important metabolic consequence that mutant cells (or individuals) are dependent not only on exogenous galactose, but also on exogenous N-acetylgalactosamine as a necessary precursor for the synthesis of glycoproteins and glycolipids. Mutations in this gene result in epimerase-deficiency galactosemia, also referred to as galactosemia type 3, a disease characterized by liver damage, early-onset cataracts, deafness and cognitive disability, with symptoms ranging from mild ('peripheral' form) to severe ('generalized' form). Multiple alternatively spliced transcripts encoding the same protein have been identified. [provided by RefSeq, Jul 2008]

Uniprot Description

Catalyzes two distinct but analogous reactions: the reversible epimerization of UDP-glucose to UDP-galactose and the reversible epimerization of UDP-N-acetylglucosamine to UDP-N-acetylgalactosamine. The reaction with UDP-Gal plays a critical role in the Leloir pathway of galactose catabolism in which galactose is converted to the glycolytic intermediate glucose 6-phosphate. It contributes to the catabolism of dietary galactose and enables the endogenous biosynthesis of both UDP-Gal and UDP-GalNAc when exogenous sources are limited. Both UDP-sugar interconversions are important in the synthesis of glycoproteins and glycolipids.