GALE recombinant protein

GALE, 1-348aa, Human, His tag, E Coli

Cat. Num. AAA203710

• Gene Names: GALE; SDR1E1
• Applications: SDS-Page
• Purity: > 95% by SDS-PAGE
• Synonyms: GALE; 1-348aa; Human; His tag; E Coli; uDP-glucose 4-epimeraes; SDR1E1.uDP-glucose 4-epimeraes; uDP-galactose 4-epimerase; Galactowaldenase; FLJ95174; FLJ97302; GALE recombinant protein

• Host: E Coli
• Purity/Purification: > 95% by SDS-PAGE
• Form/Format: Liquid. In 20mM Tris-HCl Buffer (pH 8.0) containing 10% glycerol 5mM DTT, 0.1M NaCl, and 1mM EDTA.
• Concentration: 0.5 mg/ml (determined by Bradford assay) (varies by lot)
• Sequence: MGSSHHHHHH SSGLVPRGSH MAEKVLVTGG AGYIGSHTVL ELLEAGYLPV VIDNFHNAFR GGGSLPESLR RVQELTGRSV EFEEMDILDQ GALQRLFKKY SFMAVIHFAG LKAVGESVQK PLDYYRVNLT GTIQLLEIMK AHGVKNLVFS SSATVYGNPQ YLPLDEAHPT GGCTNPYGKS KFFIEEMIRD LCQADKTWNA VLLRYFNPTG AHASGCIGED PQGIPNNLMP YVSQVAIGRR EALNVFGNDY DTEDGTGVRD YIHVVDLAKG HIAALRKLKE QCGCRIYNLG TGTGYSVLQM VQAMEKASGK KIPYKVVARR EGDVAACYAN PSLAQEELGW TAALGLDRMC EDLWRWQKQN PSGFGTQA
• Sequence Length: 348

• Applicable Applications for GALE recombinant protein: SDS-PAGE
• Antigen Species: Human
• Tag Information: His-tag
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for GALE recombinant protein

GALE, also known as uDP-glucose 4-epimeraes, is a protein that functions as the third enzyme in the Leloir pathway of galactose metabolism. It is a homodimeric epimerase found in bacterial, plant, and mammalian cells. This enzyme promotes the reverse chemical reaction, the conversion of uDP-glucose to uDP-galactose. uDP-galactose is used to build galactose-containing proteins and fats, which play critical roles in chemical signaling, building cellular structures, transporting molecules, and producing energy. Recombinant human GALE protein, fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography techniques.

Product Categories/Family for GALE recombinant protein

Enzymes & Proteases

References

Holden HM., et al. (2003) J Biol Chem. 278 (45):43885-8. ; Timson DJ., et al. (2005) FEBS J. 272 (23):6170-7. ;

NCBI and Uniprot Product Information

• NCBI GI #: 56237023
• NCBI GeneID: 2582
• NCBI Accession #: NP_000394
• NCBI GenBank Nucleotide #: NM_000403.3
• UniProt Accession #: Q14376; Q38G75; Q86W41; Q9BVE3; Q9UJB4; A0A024RAH5; B3KQ39
• Molecular Weight: 40.4 kDa (368aa) confirmed by MALDI-TOF
• NCBI Official Full Name: UDP-glucose 4-epimerase
• NCBI Official Synonym Full Names: UDP-galactose-4-epimerase
• NCBI Official Symbol: GALE
• NCBI Official Synonym Symbols: SDR1E1
• NCBI Protein Information: UDP-glucose 4-epimerase
• UniProt Protein Name: UDP-glucose 4-epimerase
• Protein Family: Galectin
• UniProt Gene Name: GALE
• UniProt Synonym Gene Names: UDP-GlcNAc 4-epimerase

NCBI Description

This gene encodes UDP-galactose-4-epimerase which catalyzes two distinct but analogous reactions: the epimerization of UDP-glucose to UDP-galactose, and the epimerization of UDP-N-acetylglucosamine to UDP-N-acetylgalactosamine. The bifunctional nature of the enzyme has the important metabolic consequence that mutant cells (or individuals) are dependent not only on exogenous galactose, but also on exogenous N-acetylgalactosamine as a necessary precursor for the synthesis of glycoproteins and glycolipids. Mutations in this gene result in epimerase-deficiency galactosemia, also referred to as galactosemia type 3, a disease characterized by liver damage, early-onset cataracts, deafness and cognitive disability, with symptoms ranging from mild ('peripheral' form) to severe ('generalized' form). Multiple alternatively spliced transcripts encoding the same protein have been identified. [provided by RefSeq, Jul 2008]

Uniprot Description

Catalyzes two distinct but analogous reactions: the reversible epimerization of UDP-glucose to UDP-galactose and the reversible epimerization of UDP-N-acetylglucosamine to UDP-N-acetylgalactosamine. The reaction with UDP-Gal plays a critical role in the Leloir pathway of galactose catabolism in which galactose is converted to the glycolytic intermediate glucose 6-phosphate. It contributes to the catabolism of dietary galactose and enables the endogenous biosynthesis of both UDP-Gal and UDP-GalNAc when exogenous sources are limited. Both UDP-sugar interconversions are important in the synthesis of glycoproteins and glycolipids.