GABARAPL1 recombinant protein

GABARAPL1 Protein, Human, Recombinant (His Tag)

Cat. Num. AAA8121710

• Gene Names: GABARAPL1; ATG8; GEC1; APG8L; ATG8B; ATG8L; APG8-LIKE
• Purity: >95% as determined by SDS-PAGE
• Synonyms: GABARAPL1; GABARAPL1 Protein; Human; Recombinant (His Tag); Human ATG8/GABARAPL1 Protein (His Tag); APG8-LIKE Protein; APG8L Protein; ATG8 Protein; ATG8B Protein; ATG8L Protein; GEC1 Protein; GABA(A) receptor-associated protein like 1; GABARAPL1 recombinant protein

• Host: E Coli
• Purity/Purification: >95% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Lys117

• Species: Human
• Predicted N Terminal: His
• Tag: N-His
• Protein Construction: A DNA sequence encoding the human GABARAPL1 (Q9H0R8)(Met1-Lys117) was expressed with a polyhistide tag at the N-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for GABARAPL1 recombinant protein

Background: ATG8, also known as GABARAPL1, is a ubiquitin-like protein that has a crystal structure. ATG8 consists of a 5-stranded beta-sheet, which is enclosed by two alpha-helices at one side and one alpha-helix at the other side and exhibits a conserved GABARAP domain. It functions in the formation of autophagosomal membranes. The transient conjugation of ATG8 to the autophagosomal membrane through a ubiquitin-like conjugation system is essential for autophagy in eukaryotes. Autophagy is induced upon nutrient depletion or rapamycin treatment and leads to the response of more than 30 autophagy-related (ATG) genes known so far, including ATG8.

References

Ohsumi Y, et al. (2004) The crystal structure of microtubule-associated protein light chain 3, a mammalian homologue of Saccharomyces cerevisiae Atg8. Genes Cells. 9(7):611-8.Geng J, et al. (2008) The Atg8 and Atg12 ubiquitin-like conjugation systems in macroautophagy. 'Protein modifications: beyond the usual suspects' review series. EMBO Rep. 9(9):859-644.Suzuki NN, et al. (2005) The crystal structure of plant ATG12 and its biological implication in autophagy. Autophagy. 1(2):119-126.

NCBI and Uniprot Product Information

• NCBI GI #: 13899219
• NCBI GeneID: 23710
• NCBI Accession #: NP_113600.1
• NCBI GenBank Nucleotide #: NM_031412.2
• UniProt Accession #: Q9H0R8; Q6FIE6; B4E0Y7
• Molecular Weight: 17,008 Da
• NCBI Official Full Name: gamma-aminobutyric acid receptor-associated protein-like 1
• NCBI Official Synonym Full Names: GABA(A) receptor-associated protein like 1
• NCBI Official Symbol: GABARAPL1
• NCBI Official Synonym Symbols: ATG8; GEC1; APG8L; ATG8B; ATG8L; APG8-LIKE
• NCBI Protein Information: gamma-aminobutyric acid receptor-associated protein-like 1; GABA(A) receptor-associated protein-like 1; GABARAPL1-a; GEC-1; early estrogen-regulated protein; glandular epithelial cell protein 1
• UniProt Protein Name: Gamma-aminobutyric acid receptor-associated protein-like 1
• Protein Family: Gamma-aminobutyric acid receptor-associated protein
• UniProt Gene Name: GABARAPL1
• UniProt Synonym Gene Names: GEC1; GEC-1
• UniProt Entry Name: GBRL1_HUMAN

Uniprot Description

GABARAPL1: is a ubiquitin-like protein that is a constituent of the ATG8-conjugation system, one of two evolutionarily conserved phosphatidylethanolamine conjugation systems necessary for the formation of the autophagosome. The human ATG8 system includes seven ubiquitin-like light chain proteins (LCPs) that are homologs of yeast LC3: MAP1LC3A, -B, -C, GABARAP, GABARAPL1, -2, and -3. Pro-LCPs are cleaved by ATG4B to expose a C-terminal glycine residue, the cytosolic LCP-I form. The exposed C-terminus is conjugated to the head group amine of phosphatidylethanolamine (PE) through an amide bond by a sequence of ubiquitination-like reactions that involves an E1 (ATG7), an E2 (ATG3), and an E3 (a complex including ATG5, ATG12, and ATG16L). The PE-congugated form (LCP-II) is tightly associated with the autophagosomal membrane. The LCP-II forms can also be delipidated by the ATG4 proteases: most of the LCPs are delipidated and liberated from the membrane before autophagosomes fuse with lysosomes. Increases cell-surface expression of kappa-type opioid receptor through facilitating anterograde intracellular trafficking of the receptor. Belongs to the MAP1 LC3 family. Interacts with GABRG2 and beta-tubulin. Interacts with KOR-1.

Protein type: Vesicle; Autophagy; Ubiquitin-like modifier; Adaptor/scaffold; Microtubule-binding

Chromosomal Location of Human Ortholog: 12p13.2

Cellular Component: dendrite cytoplasm; Golgi apparatus; microtubule; extrinsic to membrane; cytoplasmic vesicle membrane; mitochondrion; endoplasmic reticulum; autophagic vacuole; intracellular; cytosol

Molecular Function: protein binding; Tat protein binding; microtubule binding; beta-tubulin binding; GABA receptor binding

Biological Process: mitochondrion degradation; autophagic vacuole formation