Glucose 6 Phosphate Dehydrogenase (G6PD) Recombinant Protein | G6PD recombinant protein

Recombinant Glucose 6 Phosphate Dehydrogenase (G6PD)

Cat. Num. AAA2010062

• Gene Names: G6pd; G6pdx
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Glucose 6 Phosphate Dehydrogenase (G6PD); Recombinant Glucose 6 Phosphate Dehydrogenase (G6PD); G6PD recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with a N-terminal his-tag, its sequence is listed below.; MGHHHHHHSGSEF-MAEQVALSRT QVCGILREEL YQGDAFHQAD THIFIIMGAS GDLAKKKIYP TIWWLFRDGL LPEDTFIVGY ARSRLTVDDI RKQSEPFFKV TPEERPKLEE FFARNSYVAG QYDDPASYKH LNSHMNALHQ GMQANRLFYL ALPPTVYEAV TKNIQEICMS QTGWNRIIVE KPFGRDLQSS NQLSNHISSL FREDQIYRID HYLGKEMVQN LMVLRFANRI FGPIWNRDNI ACVILTFKEP FGTEGRGGYF DEFGIIRDVM QNHLLQMLCL VAMEKPASTD SDDVRDEKVK VLKCISEVET DNVVLGQYVG NPSGEGEATN GYLDDPTVPH GSTTATFAAA VLYVENERWD GVPFILRCGK ALNERKAEVR LQFRDVAGDI FHQQCKRNEL VIRVQPNEAV YTKMMTKKPG MFFNPEESEL DLTYGNRYKN VKLPDAYERL ILDVFCGSQM HFVRSDELRE AWRIFTPLLH KIDREKPQPI PYVYGSRGPT EADELMKRVG FQYEGTYKWV NPHKL
• Sequence Length: 515

• Applicable Applications for G6PD recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Rattus norvegicus (Rat)
• Expression System: Prokaryotic expression
• Residues: Met1~Leu515 (Accession # P05370) with a N-terminal his-tag
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 8393381
• NCBI GeneID: 24377
• NCBI Accession #: NP_058702.1
• NCBI GenBank Nucleotide #: NM_017006.2
• UniProt Accession #: P05370
• Molecular Weight: 60.9kDa
• NCBI Official Full Name: glucose-6-phosphate 1-dehydrogenase
• NCBI Official Synonym Full Names: glucose-6-phosphate dehydrogenase
• NCBI Official Symbol: G6pd
• NCBI Official Synonym Symbols: G6pdx
• NCBI Protein Information: glucose-6-phosphate 1-dehydrogenase; glucose-6-phosphate dehydrogenase X-linked
• UniProt Protein Name: Glucose-6-phosphate 1-dehydrogenase
• UniProt Gene Name: G6pdx
• UniProt Synonym Gene Names: G6pd; G6PD
• UniProt Entry Name: G6PD_RAT

NCBI Description

catalyzes the first rate-limiting step in the pentose phosphate pathway; may mediate peroxynitrite functions in neuroprotection against nitric oxide-mediated apoptosis [RGD, Feb 2006]

Uniprot Description

G6PD: glucose-6-phosphate dehydrogenase. A cytosolic enzyme encoded by a housekeeping X-linked gene whose main function is to produce NADPH, a key electron donor in the defense against oxidizing agents and in reductive biosynthetic reactions. G6PD is remarkable for its genetic diversity. Many variants of G6PD, mostly produced from missense mutations, have been described with wide ranging levels of enzyme activity and associated clinical symptoms. G6PD deficiency may cause neonatal jaundice, acute hemolysis, or severe chronic non-spherocytic hemolytic anemia. Two splice variant isoforms have been described. The long isoform is found in lymphoblasts, granulocytes and sperm.

Protein type: Oxidoreductase; EC 1.1.1.49; Other Amino Acids Metabolism - glutathione; Carbohydrate Metabolism - pentose phosphate pathway; Cell development/differentiation

Cellular Component: centrosome; internal side of plasma membrane; membrane; intracellular membrane-bound organelle; cytoplasm; microtubule organizing center; nucleus; cytosol

Molecular Function: identical protein binding; glucose-6-phosphate dehydrogenase activity; protein homodimerization activity; carbohydrate binding; NADP binding; glucose binding

Biological Process: pentose-phosphate shunt; erythrocyte development; erythrocyte maturation; interleukin-12 production; pentose-phosphate shunt, oxidative branch; glucose 6-phosphate metabolic process; NADP metabolic process; response to organic cyclic substance; angiotensin mediated vasodilation involved in regulation of systemic arterial blood pressure; glutathione metabolic process; substantia nigra development; regulation of neuron apoptosis; response to food; NADPH regeneration; ribose phosphate biosynthetic process; regulation of multicellular organism growth; glucose metabolic process; cytokine production; NADP biosynthetic process; cholesterol biosynthetic process; response to ethanol; angiotensin mediated vasoconstriction involved in regulation of systemic arterial blood pressure; interleukin-10 production; response to oxidative stress; pentose biosynthetic process