FYN Oncogene Related To SRC/FGR/YES Recombinant Protein | FYN recombinant protein

Recombinant FYN Oncogene Related To SRC/FGR/YES (FYN)

Cat. Num. AAA2031187

• Gene Names: FYN; SLK; SYN; p59-FYN
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: >95%
• Synonyms: FYN Oncogene Related To SRC/FGR/YES; Recombinant FYN Oncogene Related To SRC/FGR/YES (FYN); FYN recombinant protein

• Host: Host: E Coli; Source: Prokaryotic expression
• Purity/Purification: >95%
• Form/Format: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.; GCVQCKDKE ATKLTEERDG SLNQSSGYRY GTDPTPQHYP SFGVTSIPNYNNFHAAGGQG LTVFGGVNSS SHTGTLRTRG GTGVTLFVAL YDYEARTEDDLSFHKGEKFQ ILNSSEGDWW EARSLTTGET GYIPSNYVAP VDSIQAEEWYFGKLGRKDAE RQLLSFGNPR GTFLIRESET TKGAYSLSIR DWDDMKGDHVKHYKIRKLDN GGYYITTRAQ FETLQQLVQH YSERAAGLCC RLVVPCHKGMPRLTDLSVKT KDVWEIPRES LQLIKRLGNG QFGEVWMGTW NGNTKVAIKTLKPGTMSPES FLEEAQIMKK LKHDKLVQLY AVVSEEPIYI VTEYMNKGSLLDFLKDGEGR ALKLPNLVDM AAQVAAGMAY IERMNYIHRD LRSANILVGNGLICKIADFG LARLIEDNEY TARQGAKFPI KWTAPEAALY GRFTIKSDVWSFGILLTELV TKGRVPYPGM NNREVLEQVE RGYRMPCPQD CPISLHELMIHCWKKDPEER PTFEYLQSFL EDYFTATEPQ YQPGENL
• Sequence Length: 482

• Applicable Applications for FYN recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Predicted Molecular Mass: 64.3kDa
• Accurate Molecular Mass (KD): 64kDa
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Expression System: Prokaryotic expression
• Tag: two N-terminal Tags, His-tag and S-tag
• Organism Species: Homo sapiens (Human)
• Fragment: Gly2~Leu537
• Usage: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. The loss of this protein is less than 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 21618480
• NCBI GeneID: 2534
• Molecular Weight: 54,513 Da
• NCBI Official Full Name: FYN oncogene related to SRC, FGR, YES
• NCBI Official Synonym Full Names: FYN proto-oncogene, Src family tyrosine kinase
• NCBI Official Symbol: FYN
• NCBI Official Synonym Symbols: SLK; SYN; p59-FYN
• NCBI Protein Information: tyrosine-protein kinase Fyn
• UniProt Protein Name: Tyrosine-protein kinase Fyn
• Protein Family: Tyrosine-protein kinase
• UniProt Gene Name: FYN
• UniProt Synonym Gene Names: SLK
• UniProt Entry Name: FYN_HUMAN

NCBI Description

This gene is a member of the protein-tyrosine kinase oncogene family. It encodes a membrane-associated tyrosine kinase that has been implicated in the control of cell growth. The protein associates with the p85 subunit of phosphatidylinositol 3-kinase and interacts with the fyn-binding protein. Alternatively spliced transcript variants encoding distinct isoforms exist. [provided by RefSeq, Jul 2008]

Uniprot Description

Fyn: a tyrosine kinase of the Src family. Implicated in the control of cell growth. Plays a role in the regulation of intracellular calcium levels. Required in brain development and mature brain function with important roles in the regulation of axon growth, axon guidance, and neurite extension. Blocks axon outgrowth and attraction induced by NTN1 by phosphorylating its receptor DDC. Associates with the p85 subunit of phosphatidylinositol 3-kinase and interacts with the fyn-binding protein. Three alternatively spliced isoforms have been described. Isoform 2 shows a greater ability to mobilize cytoplasmic calcium than isoform 1. Induced expression aids in cellular transformation and xenograft metastasis. In squamous cell carcinoma, Fyn transduces signals from EGFR and Src and is required for cell migration and invasiveness. Activity linked to migration in a murine melanoma model. Appears to block late stage development of neuroblastoma. Mouse knockout deficient in kindling response, a model for human epilepsy.

Protein type: Protein kinase, TK; Kinase, protein; Protein kinase, tyrosine (non-receptor); EC 2.7.10.2; Oncoprotein; TK group; Src family

Chromosomal Location of Human Ortholog: 6q21

Cellular Component: extrinsic to internal side of plasma membrane; mitochondrion; postsynaptic density; plasma membrane; actin filament; nucleus; cytosol; endosome; lipid raft

Molecular Function: tubulin binding; CD8 receptor binding; ephrin receptor binding; metal ion binding; non-membrane spanning protein tyrosine kinase activity; protein binding; G-protein-coupled receptor binding; peptide hormone receptor binding; protein-tyrosine kinase activity; T cell receptor binding; phosphoinositide 3-kinase binding; glycoprotein binding; CD4 receptor binding; ATP binding

Biological Process: axon guidance; central nervous system development; nerve growth factor receptor signaling pathway; viral reproduction; T cell activation; neuron migration; protein amino acid phosphorylation; T cell receptor signaling pathway; regulation of apoptosis; regulation of cell shape; calcium ion transport; forebrain development; ephrin receptor signaling pathway; feeding behavior; negative regulation of neuron apoptosis; cell differentiation; response to drug; epidermal growth factor receptor signaling pathway; platelet activation; cell migration; phosphoinositide-mediated signaling; fibroblast growth factor receptor signaling pathway; positive regulation of I-kappaB kinase/NF-kappaB cascade; dendrite morphogenesis; activated T cell proliferation; learning; regulation of defense response to virus by virus; regulation of cell proliferation; positive regulation of phosphoinositide 3-kinase cascade; detection of mechanical stimulus involved in sensory perception of pain; response to ethanol; T cell costimulation; innate immune response; negative regulation of protein catabolic process; blood coagulation; vascular endothelial growth factor receptor signaling pathway; leukocyte migration; transmembrane receptor protein tyrosine kinase signaling pathway