Fucosyltransferase 3 Recombinant Protein | FUT3 recombinant protein

Recombinant Human Fucosyltransferase 3

Cat. Num. AAA145530

• Gene Names: FUT3; LE; Les; FT3B; CD174; FucT-III
• Purity: Greater than 80% as determined by SDS-PAGE.
• Synonyms: Fucosyltransferase 3; Recombinant Human Fucosyltransferase 3; FUT3 Human; Fucosyltransferase 3 Human Recombinant; Galactoside 3(4)-L-fucosyltransferase; Blood group Lewis alpha-4-fucosyltransferase; Lewis FT; Fucosyltransferase III; FucT-III; FUT3; FT3B; LE; CD174; Les; FUT3 recombinant protein

• Host: E Coli
• Purity/Purification: Greater than 80% as determined by SDS-PAGE.
• Form/Format: The FUT3 solution (1mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 0.4M urea and 10% glycerol.; Sterile Filtered clear solution.
• Sequence: MGSSHHHHHH SSGLVPRGSH MGSRVSRDDA TGSPRAPSGS SRQDTTPTRP TLLILLWTWP FHIPVALSRC SEMVPGTADC HITADRKVYP QADTVIVHHW DIMSNPKSRL PPSPRPQGQR WIWFNLEPPP NCQHLEALDR YFNLTMSYRS DSDIFTPYGW LEPWSGQPAH PPLNLSAKTE LVAWAVSNWK PDSARVRYYQ SLQAHLKVDV YGRSHKPLPK GTMMETLSRY KFYLAFENSL HPDYITEKLW RNALEAWAVP VVLGPSRSNY ERFLPPDAFI HVDDFQSPKD LARYLQELDK DHARYLSYFR WRETLRPRSF SWALDFCKAC WKLQQESRYQ TVRSIAAWFT.
• Sequence Length: 361

Related Product Information for FUT3 recombinant protein

Description: FUT3 Human Recombinant produced in E Coli is a single, non-glycosylated polypeptide chain containing 350 amino acids (35-361 a.a) and having a molecular mass of 40.6kDa.FUT3 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Introduction: Fucosyltransferase 3 (FUT3) catalyzes alpha-1, 3 and alpha-1, 4 glycosidic linkages which take part in the expression of Vim-2, Lewis A, Lewis B, sialyl Lewis X and Lewis X/SSEA-1 antigens. FUT3 takes part in blood group Lewis determination; Lewis-positive (Le+) individuals have an active enzyme while Lewis-negative (Le-) individuals have an inactive enzyme. FUT3 also operates on the corresponding 1, 4-galactosyl derivative, creating1, 3-L-fucosyl links.

Product Categories/Family for FUT3 recombinant protein

Enzymes

NCBI and Uniprot Product Information

• NCBI GI #: 4503809
• NCBI GeneID: 2525
• NCBI Accession #: NP_000140.1
• NCBI GenBank Nucleotide #: NM_000149.3
• UniProt Accession #: P21217; Q32NE7; Q99448; Q99449; B5U7U9; B5U7V0
• Molecular Weight: 42,117 Da
• NCBI Official Full Name: galactoside 3(4)-L-fucosyltransferase
• NCBI Official Synonym Full Names: fucosyltransferase 3 (galactoside 3(4)-L-fucosyltransferase, Lewis blood group)
• NCBI Official Symbol: FUT3
• NCBI Official Synonym Symbols: LE; Les; FT3B; CD174; FucT-III
• NCBI Protein Information: galactoside 3(4)-L-fucosyltransferase; galactoside 3(4)-L-fucosyltransferase; Lewis FT; alpha-(1,3/1,4)-fucosyltransferase; blood group Lewis alpha-4-fucosyltransferase; fucosyltransferase III
• UniProt Protein Name: Galactoside 3(4)-L-fucosyltransferase
• Protein Family: Fucosyltransferase
• UniProt Gene Name: FUT3
• UniProt Synonym Gene Names: FT3B; LE; Lewis FT; FucT-III
• UniProt Entry Name: FUT3_HUMAN

NCBI Description

The Lewis histo-blood group system comprises a set of fucosylated glycosphingolipids that are synthesized by exocrine epithelial cells and circulate in body fluids. The glycosphingolipids function in embryogenesis, tissue differentiation, tumor metastasis, inflammation, and bacterial adhesion. They are secondarily absorbed to red blood cells giving rise to their Lewis phenotype. This gene is a member of the fucosyltransferase family, which catalyzes the addition of fucose to precursor polysaccharides in the last step of Lewis antigen biosynthesis. It encodes an enzyme with alpha(1,3)-fucosyltransferase and alpha(1,4)-fucosyltransferase activities. Mutations in this gene are responsible for the majority of Lewis antigen-negative phenotypes. Multiple alternatively spliced variants, encoding the same protein, have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

FUT3: May catalyze alpha-1,3 and alpha-1,4 glycosidic linkages involved in the expression of Vim-2, Lewis A, Lewis B, sialyl Lewis X and Lewis X/SSEA-1 antigens. May be involved in blood group Lewis determination; Lewis-positive (Le(+)) individuals have an active enzyme while Lewis-negative (Le(-)) individuals have an inactive enzyme. Also acts on the corresponding 1,4-galactosyl derivative, forming 1,3-L-fucosyl links. Belongs to the glycosyltransferase 10 family.

Protein type: Glycan Metabolism - glycosphingolipid biosynthesis - lacto and neolacto series; Membrane protein, integral; Transferase; EC 2.4.1.65

Chromosomal Location of Human Ortholog: 19p13.3

Cellular Component: membrane; integral to membrane

Molecular Function: 3-galactosyl-N-acetylglucosaminide 4-alpha-L-fucosyltransferase activity; alpha(1,3)-fucosyltransferase activity; fucosyltransferase activity

Biological Process: cell-cell recognition; biopolymer glycosylation; oligosaccharide biosynthetic process; protein amino acid glycosylation