Sheep Fucosidase, Alpha L1, Tissue ELISA Kit | FUCa1 elisa kit

Sheep Fucosidase, Alpha L1, Tissue ELISA Kit

Cat. Num. AAA741823

• Gene Names: FUCA1; FUCA
• Reactivity: Sheep
• Synonyms: Fucosidase; Alpha L1; Tissue; Sheep Fucosidase; Tissue ELISA Kit; FUCa1 elisa kit

• Reactivity: Sheep

• Samples: Tissue homogenate
• Assay Type: Competitive
• Sensitivity: 0.1 ng/mL.
• Intended Uses: This FUCa1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Sheep FUCa1. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for FUCa1 elisa kit

Principle of the Assay: FUCa1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-FUCa1 antibody and an FUCa1-HRP conjugate. The assay sample and buffer are incubated together with FUCa1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the FUCa1 concentration since FUCa1 from samples and FUCa1-HRP conjugate compete for the anti-FUCa1 antibody binding site. Since the number of sites is limited, as more sites are occupied by FUCa1 from the sample, fewer sites are left to bind FUCa1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The FUCa1 concentration in each sample is interpolated from this standard curve.

Product Categories/Family for FUCa1 elisa kit

Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 119615504
• NCBI GeneID: 2517
• Molecular Weight: 53,689 Da
• NCBI Official Full Name: fucosidase, alpha-L- 1, tissue
• NCBI Official Synonym Full Names: fucosidase, alpha-L- 1, tissue
• NCBI Official Symbol: FUCA1
• NCBI Official Synonym Symbols: FUCA
• NCBI Protein Information: tissue alpha-L-fucosidase; alpha-L-fucosidase 1; alpha-L-fucosidase I; alpha-L-fucoside fucohydrolase 1
• UniProt Protein Name: Tissue alpha-L-fucosidase
• Protein Family: Tissue alpha-L-fucosidase
• UniProt Gene Name: FUCA1
• UniProt Synonym Gene Names: Nbla10230; Alpha-L-fucosidase 1
• UniProt Entry Name: FUCO_HUMAN

NCBI Description

The protein encoded by this gene is a lysosomal enzyme involved in the degradation of fucose-containing glycoproteins and glycolipids. Mutations in this gene are associated with fucosidosis (FUCA1D), which is an autosomal recessive lysosomal storage disease. A pseudogene of this locus is present on chr 2.[provided by RefSeq, Oct 2009]

Uniprot Description

FUCA1: Alpha-L-fucosidase is responsible for hydrolyzing the alpha-1,6-linked fucose joined to the reducing-end N- acetylglucosamine of the carbohydrate moieties of glycoproteins. Defects in FUCA1 are the cause of fucosidosis (FUCA1D). FUCA1D is an autosomal recessive lysosomal storage disease characterized by accumulation of fucose-containing glycolipids and glycoproteins in various tissues. Clinical signs include facial dysmorphism, dysostosis multiplex, moderate hepatomegaly, severe intellectual deficit, deafness, and according to age, angiokeratomas. Belongs to the glycosyl hydrolase 29 family.

Protein type: Glycan Metabolism - other glycan degradation; EC 3.2.1.51; Hydrolase

Chromosomal Location of Human Ortholog: 1p34

Cellular Component: lysosome; cytoplasm

Molecular Function: alpha-L-fucosidase activity; fucose binding

Biological Process: fucose metabolic process; glycosaminoglycan catabolic process; glycoside catabolic process

Disease: Fucosidosis